Home | Community | Message Board


Mushrooms.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Agar and Liquid Culture

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Potato Dextrose

Jump to first unread post. Pages: 1
Offlinegsmith
reformed lurker
Registered: 12/07/04
Posts: 204
Last seen: 11 years, 3 months
Agar question
    #3826607 - 02/24/05 11:00 AM (12 years, 1 month ago)

If one were to use Bob's Red Mill Potato Starch powder instead of boiling down potatoes when making agar, how much would one use? And is this stuff a good substitute for real potatoes/flakes? Anybody used it before? It's good in gravy BTW.


--------------------
------
this is a signature


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Agar question [Re: gsmith]
    #3826653 - 02/24/05 11:22 AM (12 years, 1 month ago)

I do not think flakes would work well , you only want the water that contains all the starches for agar mediums . The starch stuff you mentioned may work, have no idea though. Stick to know recipes for first time around , then exp with your own brew .
GL


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegsmith
reformed lurker
Registered: 12/07/04
Posts: 204
Last seen: 11 years, 3 months
Re: Agar question [Re: tripndicular]
    #3826669 - 02/24/05 11:27 AM (12 years, 1 month ago)

5g of flakes are listed in the PDA recipe as an acceptible substitute to using 150g of sliced potatoes, but i've never used them before. this isn't my first time making agar though. probably should have mentioned that. I'm just asking because I'd rather save time not having to boil the potatoes, and this stuff is cheap too.


--------------------
------
this is a signature


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Agar question [Re: tripndicular]
    #3826672 - 02/24/05 11:29 AM (12 years, 1 month ago)

Quote:

tripndicular said:
I do not think flakes would work well




then we should remove that potato flake agar recipe from the FAQ... :smirk:

the starch should work but you may have to experiment for a good mixture


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Agar question [Re: Prisoner#1]
    #3826678 - 02/24/05 11:32 AM (12 years, 1 month ago)

Sorry did not know was a substituted item , but I am lazy type and buy pre made agars(powdered form) myself so go figure :wink:
And when making dinner I hate fake textured taters :smirk:

Hey was not a wasted day... I learned something new  :thumbup: :cool: :wink:


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Edited by tripndicular (02/24/05 11:34 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegsmith
reformed lurker
Registered: 12/07/04
Posts: 204
Last seen: 11 years, 3 months
Re: Agar question [Re: tripndicular]
    #3829402 - 02/24/05 09:35 PM (12 years, 1 month ago)

I'd love some powdered MEA or something... but i need agar now-ish... i just found something that I really really want to culture... I know I've posted earlier saying that I was quitting the game but I have to preserve this specimen. I wish I had a camera to take a picture.

I was cleaning up some crap that I wanted to throw out and I found a jar that I had inoculated on this last run. I used a syringe that I knew for a fact was contaminated with black mold. I had gotten two syringes from the same batch (B+, 3rd party) and all the jars that I innocked with the first syringe contammed. So when I used this second one I just said screw it and put all 12ccs into a single quart jar. Of course it contammed, and rather quickly too. So I poured a bit of .3% h2o2 into it and put it on the back porch and forgot about it. That was about five weeks ago. Yesterday I found shrooms growing inside of it. There's six total. The biggest has a seven inch stem and a three inch cap. Impressive for in vitro... very impressive for growing out of the contams with absolutely no help from me.

I'm not going to eat it, but I want to isolate a tissue culture from the inside of the stem. Hopefully it will be clean enough for me to do this. The black mold in this jar was so thick that it literally looked like a bunch of ground up charcoal. I left it for dead. This suckers a survivor.


--------------------
------
this is a signature


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 2 months
Trusted Cultivator
Re: Agar question [Re: gsmith]
    #3832450 - 02/25/05 12:37 PM (12 years, 1 month ago)

That shit happens if you leave all your contamed jars after some time...something will sprout..



--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDamn_Skippy
Fell off theWagon

Registered: 01/25/05
Posts: 403
Re: Agar question [Re: scatmanrav]
    #3832456 - 02/25/05 12:39 PM (12 years, 1 month ago)

I was trying to eat saw those pics and almost puked


--------------------
~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 2 months
Trusted Cultivator
Re: Agar question [Re: Damn_Skippy]
    #3832669 - 02/25/05 01:33 PM (12 years, 1 month ago)

Awsome :smile:


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegsmith
reformed lurker
Registered: 12/07/04
Posts: 204
Last seen: 11 years, 3 months
UPDATE: Re: Agar question [Re: scatmanrav]
    #3886435 - 03/08/05 10:26 AM (12 years, 23 days ago)

UPDATE:

Potato starch is a bitch to work with. I still don't think I have the ammounts right and I made a couple batches. It comes out a lot thicker than the PDA I've made from scratch. It also had a lot more silt settle on the bottom. On the plus though, the tissue I dropped on it cultured nicely.

Tip: Use a food processor to fully incorporate all the ingredients. Potato starch clumps a lot and is hard to whisk apart.

I used:
500ml of boiling water
10g of Bob's Red Mill Potato Starch powder (probably too much)
10g of dextrose
5g of soy flour (had some, thought i'd use it)
5g of nutritional yeast
9g of agar


--------------------
------
this is a signature


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,122
Loc: my room
Last seen: 2 days, 12 hours
UPDATE: Re: Agar question [Re: gsmith]
    #3886456 - 03/08/05 10:37 AM (12 years, 23 days ago)

The point of using potato water is to provide micro nutrients, minerals and proteins to the agar formulation.

The potato starch lacks all of those, it is a refined product, it is no substitute at all for potato water.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegsmith
reformed lurker
Registered: 12/07/04
Posts: 204
Last seen: 11 years, 3 months
UPDATE: Re: Agar question [Re: Anno]
    #3891633 - 03/09/05 09:07 AM (12 years, 22 days ago)

well it worked... refined or not.


--------------------
------
this is a signature


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Agar question [Re: gsmith]
    #4615552 - 09/03/05 07:52 AM (11 years, 6 months ago)

I don't like boiling down potatoes either...

This is the recipy that I use...

-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast


works great!~

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Agar and Liquid Culture

Similar ThreadsPosterViewsRepliesLast post
* Agar on the cakes? DXMUser 7,951 7 09/16/04 11:48 PM
by scatmanrav
* Grocery store Agar Tek. Helltick 24,982 4 03/15/07 11:26 AM
by Terillius
* Culture storage technique
( 1 2 all )
AnnoA 25,931 39 08/23/04 07:48 PM
by YidakiMan
* multispore agar innoculation
( 1 2 3 all )
mycohomme 27,985 42 03/09/04 07:39 PM
by RogerRabbit
* :LIQUID CULTURE - THE EASY WAY:
( 1 2 3 4 5 6 all )
agar 114,456 104 11/02/08 10:29 AM
by Anno
* Isolating a strain on agar
( 1 2 3 all )
Azmodeus 26,707 42 02/28/03 09:12 AM
by Anonymous
* Agar for the old or finicky Suntzu 8,027 4 09/12/03 08:19 AM
by anotherdirtywop
* Peroxide Agar Tek (DD) Anonymous 13,732 2 11/23/01 05:51 AM
by sylo

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Ythan, Anno, Thor, Link, Seuss, geokills, Asante, Citric, FooMan
13,616 topic views. 0 members, 1 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Original Seeds Store - Cannabis Seeds
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.037 seconds spending 0.002 seconds on 15 queries.