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OfflineAzmodeus
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Isolating a strain on agar * 1
    #1313859 - 02/17/03 11:59 AM (13 years, 9 months ago)

So i made up my first ever agar last night and innoculated with gulf coast spores.  I want to do some azure agar, but thought it best to practice a little first.  So before the agar reaches the sides i need to locate the healthiest part of the agar in order to transfer and isolate it right?  Well im just wondering what the best parts of mycelium to isolate are?  Is it the rhizo growth, fuzzy growth, quickest...?  And advice on the next steps, or techniques that worked for you would also be much appreciated...

Thanks :smile:


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InvisibleJoshua
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1313886 - 02/17/03 12:08 PM (13 years, 9 months ago)

It is generally accepted that the fast rhizomorphic growth should be isolated.

Joshua


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OfflineAzmodeus
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Re: Isolating a strain on agar [Re: Joshua]
    #1313988 - 02/17/03 12:39 PM (13 years, 9 months ago)

Good to know!!! :smile:
This mycelium that i am isolating.....does it effect fruit size and body? or is it just for mycelial traits?  what is involved with "domesticating" a strain?.....



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"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


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OfflineSeussA
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Re: Isolating a strain on agar [Re: Azmodeus] * 1
    #1314204 - 02/17/03 01:48 PM (13 years, 9 months ago)

The purpose of isolating mycelium is to get a single genetic crossing from two spores rather than a racemic. An isolate will fruit at the same time. The fruit will be close to the same size and strength. Of course, you might end up isolating a nonfruiting mycelium, in which case you will not get any mushrooms at all.

To domesticate a strain, you have to grow the fruit to maturity and collect spores, usually from the best performing fruits. In this case, you want a multispore innoculation rather than an isolate to generate genetic diversity. After many generations, the fruit you are growing will become domesticated (used to growing in the parameters you have selected). This process takes a lot of time and effort.


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OfflineAzmodeus
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Re: Isolating a strain on agar [Re: Seuss]
    #1315909 - 02/18/03 06:19 AM (13 years, 9 months ago)

O.K, thanks!

What are the chances of isolating a "non-fruiting" mycelium?

And with domesticating, all you do is fruit, take the spores from the most charismatic fruit, and grow again in the exact same perameters.....how many times must one do this for it to be considered "domesticated"?


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OfflineMycolaureat
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1318882 - 02/19/03 09:48 AM (13 years, 9 months ago)

hey Azmo
If your prints was not taken from the wild, it is already domesticated.



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OfflineAzmodeus
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1321876 - 02/20/03 11:19 AM (13 years, 9 months ago)

Can anyone answer my questions?
:confused:


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Lest we forget. "


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InvisibleJoshua
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1322033 - 02/20/03 12:17 PM (13 years, 9 months ago)

does it effect fruit size and body?

It may...but on a complicated level. I think if you have more pins as rhizomorphic growth usually has you will have many small mushrooms. If you have a few pins such as cottony growth usually has you will have a few large shrooms. Other than that loose correlation, I don't think I have seen enough data to give you an informed answer.

At this point I see you have questions that can bee easily answered by reading a book. Try The Mushroom Cultivator or Growing Gourmet and Medicinal Mushrooms both by Paul Stamets.

Educate yourself.

Joshua


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InvisibleJoshua
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1322042 - 02/20/03 12:19 PM (13 years, 9 months ago)

A domesticated strain is a strain that performs well in a domestic atmosphere. You will have to see how well your isolation fruits.

IMO...PR are a non-domesticated strain. They do not follow domestic cultivation parameters as well as other more domesticated strains such as B+.

Joshua


--------------------
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Great books for inquiring minds!

"Life After Death is Saprophytic!"


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Offlinehsalf
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1322857 - 02/20/03 05:59 PM (13 years, 9 months ago)

2 out of 3


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Invisibledeanofmean
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Re: Isolating a strain on agar [Re: Joshua]
    #1323080 - 02/20/03 07:12 PM (13 years, 9 months ago)

Quote:


IMO...PR are a non-domesticated strain. They do not follow domestic cultivation parameters as well as other more domesticated strains such as B+.

Joshua




if it were, it might be as potent as B+ . lol


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InvisibleJoshua
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Re: Isolating a strain on agar [Re: deanofmean]
    #1324511 - 02/21/03 11:19 AM (13 years, 9 months ago)

Who says it is not?

Joshua


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"Life After Death is Saprophytic!"


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OfflineRaadt
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Re: Isolating a strain on agar [Re: Joshua]
    #1324526 - 02/21/03 11:26 AM (13 years, 9 months ago)

but vendor hype of course! :wink:


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineAzmodeus
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Re: Isolating a strain on agar [Re: Raadt]
    #1324550 - 02/21/03 11:32 AM (13 years, 9 months ago)

I thought that B+ was known for its lack of potency?! :confused:
At least i always hear of people eating an 1/8 to get a lv. 3 trip....

Oh, and what are the chances of isolating a non-fruiting mycelium?  slim enough for me not to worry?


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


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OfflineRaadt
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1324705 - 02/21/03 12:53 PM (13 years, 9 months ago)

with cubensis, i would say yes - very slim... this is simply a mushroom which is quite determined to reproduce.


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Invisibledeanofmean
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1324861 - 02/21/03 02:07 PM (13 years, 9 months ago)

Quote:

I thought that B+ was known for its lack of potency?! :confused:
At least i always hear of people eating an 1/8 to get a lv. 3 trip....




this was my point .i think B+ may be an example of a strain being over domesticated .i would hate to see that happen to a wonderful strain like PR ! :tongue: 
sorry about the confusion, i thought that previous statement was pretty obvious, but i guess it weren't .


Edited by deanofmean (02/21/03 02:21 PM)


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Offlinehsalf
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1325177 - 02/21/03 06:02 PM (13 years, 9 months ago)

2 out of 3
1 out of 2 if you don't isolate the ovbious "cottony" growth. Half the rhizos will be of the non-fruiting variety.
Isolate a few times and then test a few rhizo mycellium for fruiting ability.


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InvisibleZen Peddler
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Re: Isolating a strain on agar [Re: deanofmean]
    #1325284 - 02/21/03 07:07 PM (13 years, 9 months ago)

it weren't?


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Invisibledeanofmean
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Re: Isolating a strain on agar [Re: Zen Peddler]
    #1325358 - 02/21/03 08:11 PM (13 years, 9 months ago)

to me it was :smirk: 


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OfflineRaadt
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Re: Isolating a strain on agar [Re: hsalf]
    #1325503 - 02/21/03 09:47 PM (13 years, 9 months ago)

uh, have you ever isolated a strain? tormentose as hell still fruits for me np.


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Agar and Liquid Culture

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