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OfflineAnnoA
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G.Teacher clone on agar
    #348511 - 06/25/01 10:57 AM (16 years, 4 months ago)

Cloned GT fruitbody on MEA. Notice how even the cloned culture from one fruitbody produces different growth types.


Detail: rhyzomorphic growth.


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OfflineDarK_SavioR
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Re: G.Teacher clone on agar [Re: Anno]
    #348576 - 06/25/01 01:13 PM (16 years, 4 months ago)

Nice pic

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Offlinealder
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Re: G.Teacher clone on agar [Re: Anno]
    #348694 - 06/25/01 04:52 PM (16 years, 4 months ago)

i wonder why that is....
let us know what happens when you isolate the rizomorphic growh to a new plate.
alder



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OfflineSmallputrid
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Re: G.Teacher clone on agar [Re: Anno]
    #350060 - 06/27/01 03:15 PM (16 years, 4 months ago)

I would love so much to have a cloned GT, I heard they really suck at everythign except what they're supposed to do.



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Offlinemaria420
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Re: G.Teacher clone on agar [Re: Anno]
    #351745 - 06/30/01 12:51 AM (16 years, 4 months ago)

wow - i would like to try this. Can you answer a few questions for me?

so - is this what they call liquid mycelium?

where did you purchase the agar?

are all the nutrients you need already in the petrie dish?

if not - what did you ad?

please refer me to the tek on this one if you can.

thanks!

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Offlineegghead
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Re: G.Teacher clone on agar [Re: Anno]
    #351822 - 06/30/01 04:06 AM (16 years, 4 months ago)

Anno.. Could the cloned material have had GT spores on it that germinated?

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OfflineAnnoA
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Re: G.Teacher clone on agar [Re: maria420]
    #351838 - 06/30/01 05:41 AM (16 years, 4 months ago)

> so - is this what they call liquid mycelium?
no, it?s mycelium growing on a petri dish filled with malt extract agar

>where did you purchase the agar?
www.sporeworks.com, you get smaller quantities at www.mushmush.nl

>are all the nutrients you need already in the petrie dish?
yes, when it?s filled with MEA or PDYA

if not - what did you ad?
The MEA:
1 liter water
20 g agar
20 g malt extract
2 g nutritional yeast

please refer me to the tek on this one if you can.
http://www.mykoweb.com/articles/cultivation.html

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Edited by Anno on 06/30/01 05:46 AM.



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OfflineAnnoA
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Re: G.Teacher clone on agar [Re: egghead]
    #351839 - 06/30/01 05:43 AM (16 years, 4 months ago)

> Anno.. Could the cloned material have had GT spores on it >that germinated?
Hardly. I cut the cap off, then teared the stem apart and cut a piece of the flesh out at the base of the stem.

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OfflineAnnoA
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Re: G.Teacher clone on agar [Re: Anno]
    #353170 - 07/07/01 08:39 AM (16 years, 4 months ago)
Log in to view attachment

The clone on the next transfer. Still two different kinds of growth.



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OfflineJeeLiX
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Re: G.Teacher clone on agar [Re: Anno]
    #354795 - 07/14/01 08:17 PM (16 years, 4 months ago)

Awesome, nice clear photo.



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Offlineanima
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Re: G.Teacher clone on agar [Re: Anno]
    #356076 - 07/17/01 03:20 AM (16 years, 4 months ago)

Do you think a mushroom can be made up of 2 diffrent strains, or is this 1 strain with 2 manifestations?
curious.
Try getting a sector with just the rhizo growth and grow that out, ditto for the cottony stuff. Do a combo too.
this is very interesting.
One explanation might be you have both the strain of a mushroom, and a strain that composed that fuzzy mycel at the bottom your donor shroom(you know what im saying dont you) cause thats what that cottony growth reminds me of. Just a guess. Anyone ever isolated "that fuzzy mycelium"? Will it fruit?

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OfflineCACA
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Re: G.Teacher clone on agar [Re: Anno]
    #356078 - 07/17/01 03:26 AM (16 years, 4 months ago)

wow... that is beautiful

My head is hurting..
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Anonymous

Re: G.Teacher clone on agar [Re: anima]
    #356543 - 07/17/01 11:16 PM (16 years, 4 months ago)

Have you isolated the cottony growth? Doesn't it eventual rope up? Might just be trauma from the scalpel?



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OfflineAnnoA
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Re: G.Teacher clone on agar [Re: ]
    #356577 - 07/18/01 12:53 AM (16 years, 4 months ago)

Eventually the cottony growth pervaded the rhyzoomorphic after the 3rd transfer!
Strange strange. I dumped the culture.
I have a jar of rye spawn which I made with a rhyzomorphic wedge though, it shows mixed growth.

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Agar and Liquid Culture

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