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InvisibleZen Peddler
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Re: Isolating a strain on agar [Re: Raadt]
    #1326695 - 02/22/03 01:10 PM (20 years, 11 months ago)

Exactly - with cubies sometimes the most cottony shity mycelia pins like mad when the plate is full colonised. You never know until you let it pin invitro (unintentional rhyme!)


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Offlinehsalf
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Re: Isolating a strain on agar [Re: Raadt]
    #1329169 - 02/23/03 02:59 PM (20 years, 11 months ago)

With all 'our' cherished mushies? or just cubies? funny how Stamets would leave that out
nope. just an old reclusive bookworm trying to help out on a good cause. I'd never do anything illegal. now when I leave the states I may try it out. goddamn system already stole my "life(lOve)" once. She's tradin' chemicals w/the cap'in now, I hear. Fuck the 'drug' war.


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Offlinehsalf
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Re: Isolating a strain on agar [Re: Raadt]
    #1329258 - 02/23/03 03:47 PM (20 years, 11 months ago)

as an after thought do you(or anyone here) know of any tests/experiments done as to the growth rates/yeild of fruiting cotton picking(tomentose) mycellium?
I'm willing to bet, a small amount, that it prob won't be as great as the other.


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OfflineAzmodeus
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Re: Isolating a strain on agar [Re: hsalf]
    #1330695 - 02/24/03 05:01 AM (20 years, 11 months ago)

So cottony growth is better to isolate?! Or rhizo with some cottony growth as well? :confused:


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Lest we forget. "


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InvisibleG a n j a
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1330729 - 02/24/03 05:18 AM (20 years, 11 months ago)

i would go with rhizos.They seem to spread out and fill whatever medium faster.


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OfflineRaadt
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1330842 - 02/24/03 06:09 AM (20 years, 11 months ago)

rhizo, but cottony with cubes doesn't seem to matter. Rhizo are always best though, in my opinion. (They are faster growing also)


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineAzmodeus
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Re: Isolating a strain on agar [Re: Raadt]
    #1330935 - 02/24/03 06:48 AM (20 years, 11 months ago)

K, thanks guys...
Its been around 5 days, im gonna check my agar and see if its working...


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Offlinehsalf
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Re: Isolating a strain on agar [Re: Azmodeus]
    #1331711 - 02/24/03 12:27 PM (20 years, 11 months ago)

when you get to those azures make sure to ONLY use rhizo


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OfflineRaadt
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Re: Isolating a strain on agar [Re: hsalf]
    #1331775 - 02/24/03 12:46 PM (20 years, 11 months ago)

my azure culture isn't very rhizo - it's very thick satiny mycellium, and only the very tips have anything that look like rhizomorphs. Where did you hear that azure cultures have to be pure rhizo?


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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InvisibleJoshua
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Re: Isolating a strain on agar [Re: Raadt]
    #1332830 - 02/24/03 08:48 PM (20 years, 11 months ago)

It sounds like you are being azurescending.

The isolate I fruited from in my outdoor bed was cottony. I think all of this talk of rhizomorphs is interesting. I recently transfered a rhizomorphic isolation of USVI to millet, once the millet was fully colonised I then transfered a few seeds back to agar. the resulting mycelium was extremely thick and cottony.

So I thought...very interesting.

Joshua


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Offlinecanid
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Re: Isolating a strain on agar [Re: Joshua]
    #1332842 - 02/24/03 08:55 PM (20 years, 11 months ago)

josh, i have found it interesting how many are of the mind that linear mycelium is worthless and cultures must be agressively rizhomorphic to be good fruiters. people seem to forget that this is but one element that will determine how a strain fairs in propagation.


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Offlinezeronio
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Re: Isolating a strain on agar [Re: canid]
    #1332849 - 02/24/03 09:04 PM (20 years, 11 months ago)

Some mushrooms are cottony on agar and become rhizomorphic on other substrates. The reason for forming rhizomorphes could be in substrate - mushroom tries to cover a bigger area to get enough nutrients.


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OfflineRaadt
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Re: Isolating a strain on agar [Re: Joshua]
    #1333167 - 02/25/03 02:47 AM (20 years, 11 months ago)

I can't make a bed, since it wont fruit here. But I have a culture, that I isolated from a print - no idea if it's fruitable. So I was just wondering if that was useless, or if it had to be a rhizo culture, because pictures i've seen of azure cultures have not been overly rhizo either.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Anonymous

Re: Isolating a strain on agar [Re: Azmodeus]
    #1333375 - 02/25/03 04:34 AM (20 years, 11 months ago)

hey... i think TMC says that 3/4 pure cultures will not produce fruits... so you need to isolate quite a few, then test them, then use them when you know they're good. if you want a pure fruiting culture, why not clone a fruitbody grown from simple multi-spore inoculation?


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OfflineAnnoA
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Re: Isolating a strain on agar [Re: ]
    #1333408 - 02/25/03 04:45 AM (20 years, 11 months ago)

>why not clone a fruitbody grown from simple multi-spore inoculation?

That?s the preferred and easiest way, yes.


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Offlinethe spiral
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Re: Isolating a strain on agar [Re: ]
    #1334347 - 02/25/03 09:53 AM (20 years, 11 months ago)

Quote:

why not clone a fruitbody grown from simple multi-spore inoculation?





that also has the added benefit that you can use peroxidated agar, or not use agar at all.


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InvisibleZen Peddler
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Re: Isolating a strain on agar [Re: Raadt]
    #1334426 - 02/25/03 10:23 AM (20 years, 11 months ago)

I generally find that woodlovers like ps.cyanescens/azurescens/subaeruginosa tend quickly towards rhizomorphic mycelia, and even if it is cottony, its soon rhizomorphic when used to innoculate wood-based substrates.
Although Id prefer rhizos and usually stear towards them on agar, I also go on speed and primordia formation with all my mushrooms.


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Edited by Zen Peddler (02/25/03 10:24 AM)


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Offlinehsalf
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Re: Isolating a strain on agar [Re: Raadt]
    #1335069 - 02/25/03 01:49 PM (20 years, 11 months ago)

GGMM: "myc is cottony at '1st', soon silky rhizo." "the bases of the stems radiate clusters of thick white rhizos." Going by this I guess I'd wait to make sure there is a change.

Quote:

Rhizo are always best though, in my opinion. (They are faster growing also) 


 
somewhere in there is an answer to your question. :smirk:

rhizos are also best in stamet's, chilton's, McKenna bro's and the authors of the many textbooks on mycology here. So why tell someone to go at it half assed just 'cause they'll get some results?
why take the train when you can...
...walk. :confused:


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OfflineAzmodeus
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Re: Isolating a strain on agar [Re: hsalf]
    #1336944 - 02/26/03 05:40 AM (20 years, 11 months ago)

I check my petri....only two cus they are stacked...i have 8 in total. I dont see any sign of mycelial growth...but no contam either...
Its been 5 or 6 days....is it still too early?!


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Lest we forget. "


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OfflineRaadt
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Re: Isolating a strain on agar [Re: hsalf]
    #1337017 - 02/26/03 06:14 AM (20 years, 11 months ago)

well, my friends GT isolate that he's been using for a year - is NOT rhizo, it's extremely linear - yet it's the best fruiting isolate i've seen. Some clones even don't take as rhizo growth, but linear - I think you're just trying to stay away from tormentose growth. (overly fluffy/dense/slow)


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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