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Offlinegoldtop
Wild Spore

Registered: 07/28/01
Posts: 115
Loc: Sydney, Australia
Last seen: 18 years, 10 months
Agar preparation
    #918797 - 09/30/02 05:10 AM (21 years, 6 months ago)

Hi,

All the agar teks instruct to :

1. Prepare the agar
2. Sterilize it
3. Pour it into the agar plates.

Is it possible to prepare the agar, pour it into the plates first, and then put the plates into the pressure cooker to sterilize?

That way, there is less chance for contamination, as the agar AND the plates are sterilized together.



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"I want it all, I want it all, I want it all, and I want it NOW!" - Queen

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InvisibleChampion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,680
Loc: Alba
Re: Agar preperation [Re: goldtop]
    #918820 - 09/30/02 05:33 AM (21 years, 6 months ago)

yes.
just make sure to let the pressure off very slowly or the agar will bubble up and make a mess on the lids.


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hmmm........

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OfflineAnnoA
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Re: Agar preperation [Re: goldtop]
    #918834 - 09/30/02 05:39 AM (21 years, 6 months ago)

It is possible, yes, but it can be very messy.
Don?t remove the sterilized agar plates out of the pressure cooker until they are completely cold, and don?t move the pressure cooker around.
The plates prepared this way also tend to have much condensation buildup.

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Offlinehappygrins
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Re: Agar preperation [Re: Anno]
    #919002 - 09/30/02 07:40 AM (21 years, 6 months ago)

  >The plates prepared this way also tend to have much condensation buildup.

To cure this, could one stack the plates and put hot water in a coffee cup and set it on top of them? 

  Also, if you were to sterilize them together would you set them on somthing in the PC?..  I mean you woudnt want them just sitting in the water, would you?..

--happygrins :grin: 

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OfflineGreen_Velvet
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Registered: 07/26/02
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Re: Agar preperation [Re: happygrins]
    #919008 - 09/30/02 07:46 AM (21 years, 6 months ago)

In reply to:

Also, if you were to sterilize them together would you set them on somthing in the PC?.. I mean you woudnt want them just sitting in the water, would you?..




Yeah you would definately want to set them on something. They make special racks for the purpose like this. But you might be able to fabricate something. Cya

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Offlinehappygrins
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Re: Agar preperation [Re: Green_Velvet]
    #919018 - 09/30/02 07:52 AM (21 years, 6 months ago)


Thanks for that info.  That rack looks like a definate MUST HAVE then.  Anyone know of any make shift ways to get away with not using this?  Not that my friend is a cheap bastard, but someone out their has to of come of with somthing?...

--happygrins :grin:

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OfflineAnnoA
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Re: Agar preperation [Re: happygrins]
    #919022 - 09/30/02 07:54 AM (21 years, 6 months ago)

To cure this, could one stack the plates and put hot water in a coffee cup and set it on top of them?
I don?t think this would help much since you can?t take the plates out of the pressure cooker until they are cool.

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Offlinehappygrins
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Re: Agar preperation [Re: Anno]
    #919036 - 09/30/02 08:13 AM (21 years, 6 months ago)


  Right..  I mean once cooled and out of the PC, if they contain alot of condensation in them.. 

--happygrins :grin: 

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OfflineAnnoA
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Re: Agar preperation [Re: happygrins]
    #919038 - 09/30/02 08:18 AM (21 years, 6 months ago)

Don?t think it will help much once they are out of the PC....

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Offlinehappygrins
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Re: Agar preperation [Re: Anno]
    #919054 - 09/30/02 08:32 AM (21 years, 6 months ago)


  Ok.. thanks..  also, do you have any good links for a newbie to Agar?(formulas/cooking time/etc)..  do you recomend glass, or plastic petri dishes if a person planned on reusing them?..

thanks

--happygrins :grin:

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OfflineAnnoA
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Re: Agar preperation [Re: happygrins]
    #919066 - 09/30/02 08:41 AM (21 years, 6 months ago)

If you plan on reusing them, then glass.

Check out www.shroomery.org/faq/agar .

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OfflineAnnoA
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Re: Agar preperation [Re: happygrins]
    #919069 - 09/30/02 08:42 AM (21 years, 6 months ago)

Btw, you can?t pressure cook the plastic ones, the standard plastic ones are made from polystyrol, it melts in the pressure cooker.

Edited by Anno (09/30/02 01:33 PM)

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Offlinegoldtop
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Registered: 07/28/01
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Re: Agar preperation [Re: Champion des Champignons]
    #920383 - 09/30/02 07:37 PM (21 years, 6 months ago)

> just make sure to let the pressure off very slowly

How do I that? Do I just turn off the heat, and let the pressure cooker cool naturally?


--------------------
"I want it all, I want it all, I want it all, and I want it NOW!" - Queen

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Offlinegoldtop
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Re: Agar preperation [Re: Anno]
    #920392 - 09/30/02 07:39 PM (21 years, 6 months ago)

Anno,

> The plates prepared this way also tend to have much condensation buildup.

Does this result in an increased chance of contamination?


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"I want it all, I want it all, I want it all, and I want it NOW!" - Queen

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OfflineAnnoA
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Re: Agar preperation [Re: goldtop]
    #921094 - 09/30/02 06:23 PM (21 years, 6 months ago)

It can be.

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InvisibleChampion des Champignons
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Re: Agar preperation [Re: goldtop]
    #921385 - 09/30/02 10:08 PM (21 years, 6 months ago)

>Do I just turn off the heat, and let the pressure cooker cool naturally?
yes


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hmmm........

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Invisiblespores
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Re: Agar preperation [Re: goldtop]
    #923136 - 10/01/02 03:37 PM (21 years, 6 months ago)

You could just use the short 1/2 pint jars in place of petris. They work fairly well. You can sterilize the agar in the jars (no need for pouring while hot) and they're reuseable. I usually don't need more than a few agar dishes around at any given time anyway, a dozen jars is more than enough for my purposes.



DH

Edited by DethHed (10/01/02 03:46 PM)

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InvisibleZen Peddler
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Re: Agar preperation [Re: goldtop]
    #926479 - 10/02/02 09:27 PM (21 years, 6 months ago)

i pour my plates in a glove box and from about 200 plates only two have ever contaminated - its easy


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OfflineMsPacMan
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Registered: 10/05/02
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Re: Agar preperation [Re: Zen Peddler]
    #969570 - 10/17/02 03:10 PM (21 years, 6 months ago)

there are no agar teks, i checked out the FAQ and it doesn't give step by step.  i have my agar ready and wrapped, if i want to inject spores, is it as simple as innoculating it?
im going to innoc 2 and stick a little myc. in the others.  :blush:

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OfflineAnnoA
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Re: Agar preparation [Re: MsPacMan]
    #969616 - 10/17/02 03:26 PM (21 years, 6 months ago)

>there are no agar teks, i checked out the FAQ and it doesn't give step by step.

Ever heard of links?

Look, at the bottom of this faq,
http://shroomery.org/faq/faq.php?display=faq&nr=99&catnr=11&prog=1&lang=en
there are some so called Links, if you click on those "links". then a new window pops up and you can then read the information given in this other window....

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