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Amazon Shop for: ½ Pint Jars, Agar

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OfflineCubieman420
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Polyfill, tyvek or filter discs?
    #4567775 - 08/22/05 09:20 PM (11 years, 3 months ago)

Which method do YOU think is the best and why? I am of course referring to the different methods of allowing air exchange in your jars without introducing contaminants. Also, with the tyvek or filter discs (the type mycosupply has), is it best to forget the lid all-together and just use the ring? Thanks...


--------------------
"...now waters run free, no more fish in the sea..."
1983-2004


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Offlinekingboomer
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Re: Polyfill, tyvek or filter discs? [Re: Cubieman420]
    #4567797 - 08/22/05 09:25 PM (11 years, 3 months ago)

:mushroom2:  Tyvek's easier to work with than polyfill, but their filtering capacities are comparable.  I like to use tyvek.  I personally believe that you should use the lid rather than just the ring.  You want a good seal to keep out as much contams as possible while the sub is colonizing.  :mushroom2:


-Kingboomer


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take me to heaven..
window in time/
through it I'll fly...

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OfflineNashbar
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Re: Polyfill, tyvek or filter discs? [Re: Cubieman420]
    #4567802 - 08/22/05 09:25 PM (11 years, 3 months ago)

tyvek with holes in the lid is recommended (I think). never had problems with this method.


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:pacman: :mushroom2:



Edited by Nashbar (12/13/06 07:30 PM)


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OfflineOddish
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Re: Polyfill, tyvek or filter discs? [Re: Cubieman420]
    #4567851 - 08/22/05 09:34 PM (11 years, 3 months ago)

Quote:

Cubieman420 said:
Which method do YOU think is the best and why? I am of course referring to the different methods of allowing air exchange in your jars without introducing contaminants. Also, with the tyvek or filter discs (the type mycosupply has), is it best to forget the lid all-together and just use the ring? Thanks...




Sometimes using just tyvek and the ring allows too much gas exchange. I've seen the top portion of a jar not colonize as well because the substrate has become more dry due to not having a lid.


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InvisibleFungusMan
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Re: Polyfill, tyvek or filter discs? [Re: Oddish]
    #4567857 - 08/22/05 09:36 PM (11 years, 3 months ago)

Yeah. I just use coffee filters on top of my lid. Works so far.


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OfflineAiko Aiko
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Re: Polyfill, tyvek or filter discs? [Re: FungusMan]
    #4567945 - 08/22/05 09:57 PM (11 years, 3 months ago)

You could try a micro-pore tape over the holes.


--------------------
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InvisibleOldSpice
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Re: Polyfill, tyvek or filter discs? [Re: Cubieman420]
    #4568116 - 08/22/05 10:37 PM (11 years, 3 months ago)

I use both..I put my tyvek on the lid and some polyfill under the holes in the lid where i inoculate, Doing it this way is double protection against contams
If you just use tvyek and tape your innoc hole it still can let contams in especially when you shake your jars, Think about it
Its cut my qt jar contam rate to non existant pretty much, The only way my jars contam is if its a bad syringe :smirk:


Edited by Eatmydickcom (08/22/05 10:47 PM)


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OfflineAiko Aiko
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Re: Polyfill, tyvek or filter discs? [Re: OldSpice]
    #4568135 - 08/22/05 10:40 PM (11 years, 3 months ago)

I mean still using tyveck under the lid. So it would be tyveck, lid, ring, tape. I think the holes in the tape would be smaller than the holes in the polyfill. No?


--------------------
Man says, "God, show me and I will believe." God says, "Believe and I will show you."


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Offlinehyphae
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Re: Polyfill, tyvek or filter discs? [Re: Oddish]
    #4568157 - 08/22/05 10:45 PM (11 years, 3 months ago)

Quote:

Oddish said:
Quote:

Cubieman420 said:
Which method do YOU think is the best and why? I am of course referring to the different methods of allowing air exchange in your jars without introducing contaminants. Also, with the tyvek or filter discs (the type mycosupply has), is it best to forget the lid all-together and just use the ring? Thanks...




Sometimes using just tyvek and the ring allows too much gas exchange. I've seen the top portion of a jar not colonize as well because the substrate has become more dry due to not having a lid.




Word! My experience as well. Polyfil has it's uses but not as a lid filter IMHO. Tyveks free and works like a charm and when used in combination with the lid allows the jars to be shaken when doing grains.
Oh ya do yourselves a favor forget the tape! It doesn't make for a healthy culture period.


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
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Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleFooManM
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Re: Polyfill, tyvek or filter discs? [Re: Aiko Aiko]
    #4568172 - 08/22/05 10:47 PM (11 years, 3 months ago)

You could go tyvek, lid, tyvek and then put the ring over all 3. Of course you would need to have holes drilled in the lid for inoculation. I like Tyvek the best out of the 3 because it's free.


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InvisibleOldSpice
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Re: Polyfill, tyvek or filter discs? [Re: Aiko Aiko]
    #4568183 - 08/22/05 10:50 PM (11 years, 3 months ago)

Re read my post i edited it, Hyphae is right about just using tyvek your jars would dry out really fast not using the lid


--------------------
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OfflineAiko Aiko
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Re: Polyfill, tyvek or filter discs? [Re: OldSpice]
    #4568238 - 08/22/05 11:02 PM (11 years, 3 months ago)

Either way, after you innoc., your going to have to cover those holes. I think two layers of tyvek might start restricting gas exchange.


--------------------
Man says, "God, show me and I will believe." God says, "Believe and I will show you."


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Invisibleagar
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Re: Polyfill, tyvek or filter discs? [Re: Cubieman420]
    #4568239 - 08/22/05 11:02 PM (11 years, 3 months ago)



--------------------


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OfflineAiko Aiko
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Re: Polyfill, tyvek or filter discs? [Re: agar]
    #4568258 - 08/22/05 11:05 PM (11 years, 3 months ago)

So no tape, no poly. Just a couple coffee filters. Nice. You can always count on Mr. agar :grin:


--------------------
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InvisibleOldSpice
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Re: Polyfill, tyvek or filter discs? [Re: agar]
    #4568268 - 08/22/05 11:07 PM (11 years, 3 months ago)

I use poly on top of my tyvek Agar and when innocing i am going thru tyvek and poly, I allways tape the nnoc hole anyway
Nice example..Wht kind of camera are you using?..


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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OfflineAiko Aiko
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Re: Polyfill, tyvek or filter discs? [Re: OldSpice]
    #4568313 - 08/22/05 11:17 PM (11 years, 3 months ago)

Do you tape over the poly? And it makes a good seal?


--------------------
Man says, "God, show me and I will believe." God says, "Believe and I will show you."


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InvisibleOldSpice
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Re: Polyfill, tyvek or filter discs? [Re: Aiko Aiko]
    #4568378 - 08/22/05 11:27 PM (11 years, 3 months ago)

Yeah i tape my innoc hole but i probably dont have to, My a drive floppy is down so i cant post pics but as soon as i get it replaced ill post pics of how i do it :smirk:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Re: Polyfill, tyvek or filter discs? [Re: Cubieman420]
    #4568427 - 08/22/05 11:36 PM (11 years, 3 months ago)

Some people swear by poly fill, some love the tyvek. For ease of use, tyvek is the way to go. If you have a hard time getting tyvek, poly fill works perfectly well if you know how to construct a proper filter with it.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflineCubieman420
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Re: Polyfill, tyvek or filter discs? [Re: Psychoslut]
    #4569298 - 08/23/05 02:57 AM (11 years, 3 months ago)

I would really like to hear more about the synthetic filter discs. One time I use the discs along with the lid, and used polyfill for the innoc hole which was about 1/2" wide. The jar colonized so slow that we just threw them out. It seemed like that was not enough air exchage for sure. So I would guess that tyvek lets more air through? And also, what about using bandaids over innoc points on a jar w/o the lid and just a filter disc/polyfill?


Failed me.... :thumbdown:
(filter disc)


Link:

http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=SFDI01


--------------------
"...now waters run free, no more fish in the sea..."
1983-2004


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Amazon Shop for: ½ Pint Jars, Agar

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