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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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OfflineShroomNoob03
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Synthetic Filter Discs, Jar Preparation and Sterilization
    #4632785 - 09/08/05 03:27 AM (11 years, 2 months ago)

Primary Materials:
? Pint Plastic Canning Jars (One-Piece, Polypropylene)
50mm Synthetic Filter Discs
Substrate (Vermiculite, Brown Rice Flour)

I. Jar Preparation -
a. Drill a 1 in. hole in the center of the lid for Synthetic Filter Disc:
i. Is a 1 in. hole too large? Or unnecessary?
ii. What type of drill bit should be used?

b. Drill four small inoculation holes along the outer edge of the lid.
c. Seal each of the four inoculation holes with electrical tape.
d. Fill jars with substrate and a 1 in. layer of dry vermiculite.

e. Insert Synthetic Filter Disc:
i. Where should one place a disc when using one-piece lids?
ii. If beneath the lid, how would one keep the disc in place?
iii. What effect would water have on the disc (ex. sterilizing in boiling water)?
iv. How would one prevent water from seeping into the dry vermiculite layer?

f. Assemble Jars.

Any advice on the matter would be greatly appreciated.

Thanks,
ShroomNoob03


--------------------
In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.

-Carl Sagan


Edited by ShroomNoob03 (09/08/05 03:30 AM)


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InvisibleFooManM
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Re: Synthetic Filter Discs, Jar Preparation and Sterilization [Re: ShroomNoob03]
    #4633049 - 09/08/05 07:30 AM (11 years, 2 months ago)

I would have gone with the free Tyvek envelopes from the post office myself and just cut them to size. This thread may help you out though.


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InvisibleFungusMan
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Re: Synthetic Filter Discs, Jar Preparation and Sterilization [Re: FooMan]
    #4633093 - 09/08/05 08:34 AM (11 years, 2 months ago)

I dont even fool with filter discs or stuff like that anymore. My supplies, canning jar, aquarium air tubing, polyfill, and gasket maker. Self healing injection sites are the shizznitty.


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OfflineShroomNoob03
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Re: Synthetic Filter Discs, Jar Preparation and Sterilization [Re: FungusMan]
    #4633503 - 09/08/05 12:46 PM (11 years, 2 months ago)

Self healing injection sites? How does that work?

If one doesn't use synthetic filter discs, how else could one achieve a good gas exchange with One-Piece plastic lids?

Thanks again,
ShroomNoob03


--------------------
In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.

-Carl Sagan


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OfflineDarkDisciple
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Registered: 08/18/05
Posts: 46
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Re: Synthetic Filter Discs, Jar Preparation and Sterilization [Re: ShroomNoob03]
    #4633508 - 09/08/05 12:50 PM (11 years, 2 months ago)

double stacked coffee filters screwed on and holes in the lid


--------------------
Pride is a Fool's Version of Wisdom
-Anonymous

"Don't study the idea to death with experts and committees. Get on with it and see if it works."

-Ken Iverson


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InvisibleFooManM
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Re: Synthetic Filter Discs, Jar Preparation and Sterilization [Re: ShroomNoob03]
    #4633545 - 09/08/05 01:09 PM (11 years, 2 months ago)

Quote:

ShroomNoob03 said:
If one doesn't use synthetic filter discs, how else could one achieve a good gas exchange with One-Piece plastic lids?





Don't get stuck on the filter discs. Tyvek and polyfill work the same and provide gas exchange. Fungusman is using polyfill inside of the tube you see sticking out of the jar. See it? This provides enough gas exchange. In your case, I would drill two holes in the lid. 1 larger hole for air exchange and 1 smaller hole just big enough for your syringe to inject through. Put the filter underneath the lid. Cover the tops of the jars with foil when you PC so that water doesn't drip into the holes. When you inject the jars, you will pierce the filter. After you inject the jar, cover the injection hole with tape. DONE!


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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