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OfflineFOAF
Stranger
Registered: 06/28/01
Posts: 12
Last seen: 21 years, 8 months
Filter disc in place of verm seal?
    #690617 - 06/20/02 01:40 PM (22 years, 28 days ago)

So i just made up 12 1/2 pint jars of BRF/VERM and didnt use the top dry layer of verm, instead I just sealed in filter discs above it...I then sterilized the jars aftre the discs were inplace and then innoculated THROUGH the disks... are my jars in trouble?


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If youve never stared off into the distance
then youre life is a shame
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Offlinebassplayer74
old hand

Registered: 11/23/01
Posts: 906
Loc: Tennessee
Last seen: 13 years, 8 months
Re: Filter disc in place of verm seal? [Re: FOAF]
    #690629 - 06/20/02 01:45 PM (22 years, 28 days ago)

It will prolly be ok but its much better and easier to just put the dry verm layer on. Thats what the tek calls for and thats what works best. So why not just do that? Its cheaper too.


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If you will it, it is no dream.

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Offlinevatoloco
Puppet Hunter -DBK
Registered: 01/30/02
Posts: 7,653
Last seen: 19 years, 9 months
Post deleted by MOE THE MAD SCIENTIST [Re: FOAF]
    #690816 - 06/20/02 02:59 PM (22 years, 28 days ago)


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InvisibleDrD525
what'shappening?!
Registered: 12/30/01
Posts: 182
Re: Filter disc in place of verm seal? [Re: vatoloco]
    #690874 - 06/20/02 03:21 PM (22 years, 28 days ago)

Can you give some more detail on your "three layer" system? And who is that person on your sig?!

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InvisibleFrog31337
Stranger

Registered: 06/17/02
Posts: 779
Loc: Midwest, US
. [Re: FOAF]
    #691912 - 06/21/02 05:26 AM (22 years, 28 days ago)

.

Edited by Frog31337 (08/26/02 06:29 AM)

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OfflineFOAF
Stranger
Registered: 06/28/01
Posts: 12
Last seen: 21 years, 8 months
Re: Filter disc in place of verm seal? [Re: Frog31337]
    #691921 - 06/21/02 05:32 AM (22 years, 28 days ago)

I didnt use te Verm seal or the Ramsey seal because as shitty as it is to admit I had these filter disks I bought and wanted to use them. As they had been sitting arond forever. I thought if I put in the verm and then the filter disck on top of that it would not let the fd's do their job or something. Im quite new to this so that seemed reasonable to me. It's not worth it to open them up now and put the verm in is it? As it would introduce airborne contams right? I kept the metal caps on and have the FD's in plac so i guess that's 2 layers of protection...


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====================================
If youve never stared off into the distance
then youre life is a shame
===================================

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Offlinebassplayer74
old hand

Registered: 11/23/01
Posts: 906
Loc: Tennessee
Last seen: 13 years, 8 months
Re: Filter disc in place of verm seal? [Re: FOAF]
    #691990 - 06/21/02 06:15 AM (22 years, 28 days ago)

Never open a jar after sterilization unless it is 100% colonized.


IMO, if you have a 3/4" dry verm layer and follow normal sterile technique, this is sufficient.


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If you will it, it is no dream.

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InvisibleMushroom_Madness
enthusiast
Registered: 01/10/02
Posts: 261
Loc: Quaint New England Villag...
Re: Filter disc in place of verm seal? [Re: FOAF]
    #692302 - 06/21/02 09:23 AM (22 years, 28 days ago)

Everything should be fine. Our researchers use Contamination Guards to cover their jars without a verm layer (when working with cakes) and they have not had one contamination problem. But every one can not expect to have the same results in this hobby. I would recommend using a verm layer on some of your jars, and the others without a verm layer.

If you do this, let us know what the results are.

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