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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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CLEAN INOCULATIONS
    #4489263 - 08/03/05 10:39 AM (18 years, 8 months ago)

Assuming your syringes are uncontaminated, as well as the jar & content you are inoculating :rolleyes: :confused:.

It is SIMPLE to INOCULATE cleanly, if you can follow instructions?

Simply use a sterile gauze pad ( medical type @ any drug store by the box for cheap).

Drip some 70% alc on it - until fairly saturated.

Uncap the needle off the syringe & WRAP the saturated pad around it.

Hold the pad in place with 2 fingers.

Place the base of the pad against the hole in the jar lid (which sanitizes the hole & area around it), then push the needle downward - through the pad, into the jar & inject - while still holding the pad in place over the hole.

After completing the injection, withdraw the needle, BACK UP INTO THE ALC saturated PAD.

Set syringe aside.      Tape over the hole.

Proceed to next jar & repeat.

This simple procedure does not allow the needle to come into contact with open air that carries contaminates.


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Invisiblebackupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: CLEAN INOCULATIONS [Re: agar]
    #4489342 - 08/03/05 11:05 AM (18 years, 8 months ago)

yeah, nice agar. i think there are some that need to pay attention to how they are innc'ing and quit blaming vendors for there contams. sure there can be contam'd syringes from everyone, but when you have a bunch of them of different strains and say they are all contam'd then there obviously is something else wrong. that is just imo though.

peace

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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 3 months
Trusted Cultivator
Re: CLEAN INOCULATIONS [Re: backupwards]
    #4489373 - 08/03/05 11:12 AM (18 years, 8 months ago)

Everyone should notice that flaming the needle is left out because its not needed...I only flame needles on syringes I was sent, and then only once, the first time I use it. Otherwise I do as agar explained. I get these alcohol pads at the drug store though, guaze presoaked in alcohol. And I still havnt found 3M tape locally, so I use bandaids.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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Invisibleagar
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: CLEAN INOCULATIONS [Re: scatmanrav]
    #4489723 - 08/03/05 12:22 PM (18 years, 8 months ago)

http://cgi.ebay.com/MICROPORE-TAPE-WHITE...1QQcmdZViewItem

12 rolls @ 1 inch wide & 30 feet long

:grin: :thumbup:


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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