Home | Community | Message Board


Lil Shop Of Spores
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Aquarium Heater

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
InvisibleDickhead
2 Times
Female User Gallery
Registered: 01/24/05
Posts: 28,769
Loc: groin
1 Jar Half Colonized / 10 others - not a spot of white???
    #4250076 - 06/02/05 10:46 PM (11 years, 6 months ago)

???

This happen regularly??

My temps and sterile techniques are to the letter this time. Still, Only one jar is colonizing... and is half done. Been about a week.


--------------------
Multiplied


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegrow
i ^
Registered: 04/09/05
Posts: 172
Last seen: 10 years, 4 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Dickhead]
    #4250092 - 06/02/05 10:52 PM (11 years, 6 months ago)

same thing happened to me my first time incubating. I figured I put a little more spore juice in that 1 jar. they still all colonized in under 12 days. ---[grain]---


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJihadISfreind
Stranger

Registered: 06/02/05
Posts: 203
Last seen: 11 years, 5 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: grow]
    #4250104 - 06/02/05 10:56 PM (11 years, 6 months ago)

maybe u didnt put inuff injection spots in the jar lid


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAkashaPrinciple
Think ForYourself
Male User Gallery

Registered: 05/19/05
Posts: 289
Last seen: 3 years, 6 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: JihadISfreind]
    #4250120 - 06/02/05 11:00 PM (11 years, 6 months ago)

Quote:

JihadISfreind said:
maybe u didnt put inuff injection spots in the jar lid




:banbanban:

Sorry to be a dickhead, but seriously, this guy hasnt even got a clue how to do anything relating to cultivation, and hes now answering questions. Please man, try not to post on here untill you have done a shitload of reading, its really annoying. I understand ur a noob, i am too, but take the time to educate yourself first before you try and educate others in this area.


--------------------
And in my darkest moment, fetal and weeping. The moon tells me a secret.
"My confidant, As full and bright as I am, this light is not my own,
A million light reflections pass over me, It's source is bright and endless"


TOOL - 'Reflection'




Edited by AkashaPrinciple (06/02/05 11:01 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAkashaPrinciple
Think ForYourself
Male User Gallery

Registered: 05/19/05
Posts: 289
Last seen: 3 years, 6 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Dickhead]
    #4250148 - 06/02/05 11:06 PM (11 years, 6 months ago)

Quote:

throy said:
???

This happen regularly??

My temps and sterile techniques are to the letter this time. Still, Only one jar is colonizing... and is half done. Been about a week.




Hi, im not experienced with this at all, so its just based on my reading. But from what ive heard it can take upto 2 weeks, so id just be patient, might just be taking a while to start.


--------------------
And in my darkest moment, fetal and weeping. The moon tells me a secret.
"My confidant, As full and bright as I am, this light is not my own,
A million light reflections pass over me, It's source is bright and endless"


TOOL - 'Reflection'




Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegrow
i ^
Registered: 04/09/05
Posts: 172
Last seen: 10 years, 4 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: AkashaPrinciple]
    #4250305 - 06/02/05 11:37 PM (11 years, 6 months ago)

I second that Akasha, just this morning he had 20 posts, now he has 106... Fuck, how many questions has he answered? I hope no one takes his advice before he is banned.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMiscusi
Upstate Chillin'
Male User Gallery

Folding@home Statistics
Registered: 07/02/04
Posts: 2,380
Loc: Canadas eh? Flag
Last seen: 16 days, 6 hours
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: grow]
    #4250347 - 06/02/05 11:46 PM (11 years, 6 months ago)

I think you should do a few grows b4 u answer questions or atleast KNOW wtf you are talking aobut not relay sumtihng you READ


--------------------
:hypno: :merkaba: :peace: My Trade List (2-12-16) :peace: :merkaba: :hypno: 
                                                       
:mushroom2: :syringe: +++ AMU Q&A and TEK LIST +++ :syringe: :mushroom2:
:trippinbawelz:
:nyan: Cult Guide :nyan:
:discorex: :dancingbacon: :carlton:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAkashaPrinciple
Think ForYourself
Male User Gallery

Registered: 05/19/05
Posts: 289
Last seen: 3 years, 6 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: grow]
    #4250552 - 06/03/05 12:39 AM (11 years, 6 months ago)

Quote:

grow said:
Fuck, how many questions has he answered? I hope no one takes his advice before he is banned.




Same here. I have no idea how many questions hes answered, but i think the majority of his posts are from the threads he created, he made like 10 of em in a few hrs, rediculous. :nonono:


--------------------
And in my darkest moment, fetal and weeping. The moon tells me a secret.
"My confidant, As full and bright as I am, this light is not my own,
A million light reflections pass over me, It's source is bright and endless"


TOOL - 'Reflection'




Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemyndreach
philosopher
Male User Gallery

Folding@home Statistics
Registered: 08/07/04
Posts: 2,368
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: AkashaPrinciple]
    #4250618 - 06/03/05 12:55 AM (11 years, 6 months ago)

Yeah...wait some more, it can take a while sometimes.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineattar
journeyman
Registered: 10/20/02
Posts: 76
Last seen: 11 years, 6 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: myndreach]
    #4250695 - 06/03/05 01:10 AM (11 years, 6 months ago)

Perhaps whatever was in the syringe was not well distributed?
You may get some growth if you wait,
but if you have more empty jars,
and something with which to innoc,
then start some more.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSilly_Cyben
not-quite-newbie

Registered: 04/11/05
Posts: 183
Loc: USA
Last seen: 8 years, 8 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Dickhead]
    #4250710 - 06/03/05 01:14 AM (11 years, 6 months ago)

I had two jars out of 24 with no growth. Others colonized at different rates. I suspect the reason was I didn't vigorously shake the spore syringes between each and every inoculation.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDickhead
2 Times
Female User Gallery
Registered: 01/24/05
Posts: 28,769
Loc: groin
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Silly_Cyben]
    #4250896 - 06/03/05 02:02 AM (11 years, 6 months ago)

Not at all sure whats going on here... I was pretty good with my innoculating technique too.

Though I did re-sterilize the needle tip with alcohol once through the batch.

Was this a mistake?? Could that have "killed" the spores?

None the less I'm excited about the one jar I have and the lessons in sterilization have been golden.

Besides, Im likely just being impatient.


--------------------
Multiplied


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSilly_Cyben
not-quite-newbie

Registered: 04/11/05
Posts: 183
Loc: USA
Last seen: 8 years, 8 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Dickhead]
    #4251242 - 06/03/05 04:27 AM (11 years, 6 months ago)

I flamed the needle and dipped it in alcohol between each jar, so I don't think your dipping it in alcohol was the issue. I've had inconsistent results with my liquid cultures also. All of my jars that have grown myc showed growth within 5 days, regardless of the strain, and I have the following strains growing now: PR, A, EQ, KS, CA, Z, PF, OI, and KC. My second batch of jars are showing better all-round colonization than the first batch, and I think this is because I took care to shake the syringe between each and every inoculation, not just between jars.

I finally sprung for a TiT setup, and after some adjustments, I'm getting consistent temps. Before I was using ice chests, one with an aquarium heater bomb (heater mounted in a plastic water bottle full of water) and one with a large hot pad under the chest. Temps were all right with those, but there were sometimes variations of several degrees depending on ambient temp. I spawned my first PR cake in h poo two days ago, and when I peeked at it today, I was gratified to see that cubes LOVE poo, just like the big boys and girls tell us. Thanks, again, to you experienced growers who take the time and trouble to help us noobies.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDickhead
2 Times
Female User Gallery
Registered: 01/24/05
Posts: 28,769
Loc: groin
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Silly_Cyben]
    #4282166 - 06/11/05 01:59 AM (11 years, 5 months ago)

Many days later still nothing on the rest of teh jars.

Any advice?

I have some Liquid culture.

Should I re-inoculate with that?


--------------------
Multiplied


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineOnetwothree
This is MajorTom
 Arcade Champion: Space Runner

Registered: 02/25/05
Posts: 168
Last seen: 7 years, 10 days
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Dickhead]
    #4282572 - 06/11/05 06:48 AM (11 years, 5 months ago)

Well... I know I hit up 18 grain jars (with LC though, but sterile steps are the same)... and all I did before-hand was flame the needle, and wipe it with an alcohol dipped cotton-swab, and then did all 18 jars.

How many CC's did you put in each jar? I know if you put too much juice in a jar it can mess things up (so I've heard)... What temps are the jars at? The INSIDE of the jar should be near 82-88F... like, the CORE of your jars should be that hot, not just the outside, you know? How humid?


--------------------
Free Spore Ring Canada


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinethashaman
Biophilosopher

Registered: 11/09/04
Posts: 161
Last seen: 6 years, 8 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Dickhead]
    #4282727 - 06/11/05 10:05 AM (11 years, 5 months ago)

Ok, here's the deal. Many things that everyone has said in this post have been correct. All of them pretty much. I find that the jars colonize better in 80-86 degrees F. 88 sounds a little high to me but hey, what's two degrees. YES I ALSO THINK THAT EVEN NEWBIES SHOULD STOP GIVING OUT DIPSHIT ADVICE BEFORE EDUCATING THEMSELVES OR EXPERIENCING CULTIVATION TEKS. I am not familiar with Liquid Culture but, let me just say this. It has only been a week. HAVE PATIENCE. I recommend putting your colonizing jars somewhere dark and warm, and NOT FUCKING TOUCHING THEM for another week and a half at least! Don't look, don't fan, don't kiss, or smell them, just let em' do thier thang' man! I'd be willing to bet when you sneak a gander in a week and a half, more than 50% will have some sort of growth. And if not, well then you can message me and tell ME to go educate myself further.  Otherwise, you can just send me half of what your yield is :smile:. JK.  And remember, just think like the mycelium. And if you DO figure out a way tried and true not to look at your mycelium for X amount of days, let me know, because I am a huge fucking hipocrit! Good luck.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDickhead
2 Times
Female User Gallery
Registered: 01/24/05
Posts: 28,769
Loc: groin
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: thashaman]
    #4282913 - 06/11/05 11:32 AM (11 years, 5 months ago)

2 weeks.

84 degrees.

Dark container.

1cc each jar.

Fanning/checking once every 2 days.

1 jar almost completely colonized.

2 suffered contams and were removed.

the rest are still naked and sleeping.


--------------------
Multiplied


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekeith813
living in the70s
Registered: 05/17/05
Posts: 64
Last seen: 11 years, 5 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Dickhead]
    #4282966 - 06/11/05 11:52 AM (11 years, 5 months ago)

um well... it doenst hurt to check your jars once a day for contams, because if one of your jars does contam, you would need to take it out and toss it immediately... waiting for your jars takes patience but it will come.. if you dont see any growth within 2 weeks of inoculation then you can start worrying, for now just relax... :smile:


--------------------
whats the difference between sweat and spit?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDickhead
2 Times
Female User Gallery
Registered: 01/24/05
Posts: 28,769
Loc: groin
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: keith813]
    #4282970 - 06/11/05 11:54 AM (11 years, 5 months ago)

Its been 2 weeks today yo. 1 jar looks fantastic.

not a worry... just looking to see if there is anything I can do.


--------------------
Multiplied


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekeith813
living in the70s
Registered: 05/17/05
Posts: 64
Last seen: 11 years, 5 months
Re: 1 Jar Half Colonized / 10 others - not a spot of white??? [Re: Dickhead]
    #4282976 - 06/11/05 11:56 AM (11 years, 5 months ago)

theres really nothing u can do, its really weird but that sorta shit happends, best thing you can do is just try again :smile:


--------------------
whats the difference between sweat and spit?


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Amazon Shop for: ½ Pint Jars, Aquarium Heater

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* rye jars half colonized whie pf jars oing OK kazmierczakkm 660 3 01/19/09 01:14 PM
by kazmierczakkm
* What to do with a bunch of half colonized agar plates? scatmanrav 718 3 07/25/04 05:35 PM
by scatmanrav
* Jars arent colonizing! Help me please! TheHauntingSoul 711 15 08/29/07 06:54 AM
by veda_sticks
* Help- Bottoms of jars not colonizing iwannarock999 2,129 15 01/13/12 10:24 PM
by mesoamerican
* jars not colonizing! indianaroller 943 12 11/11/04 11:55 AM
by indianaroller
* PF Tek - 10 Jars - Early Pinning (1st Grow) [Update+Bump] options 2,740 14 04/10/09 06:48 PM
by options
* 5 out of 6 jars fully colonized... question about the other jar. danknugz81 1,556 2 04/24/08 07:49 PM
by danknugz81
* half colonized jars stara 1,065 14 06/29/03 10:01 PM
by ph_plus

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
1,247 topic views. 24 members, 100 guests and 23 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Fairy Ring Spores
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.061 seconds spending 0.003 seconds on 16 queries.