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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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18 Jars no colonization WHAT GIVES
    #433972 -

Ok, my friend's friend's friend pc'd 18 rye jars, two days later innoculated with eq's spore water. 5 days later still no sign of colonization. The next day he decides to take 8 of them and use an agar wedge on each. Then on day 7 he takes 4 more and uses a tapalpa syringe on those 4. So there's 6 With the EQ juice still sitting there, 8 with eq juice and an agar wedge, 4 with tapalpa and eq juice. It's now day 10 and here's the deal, NO growth on the EQ jars, the agar wedges stopped growing, and no sign of the tapalpa growth(mind you it's only been 3 days since innoculation with that. This guy has never had a problem EVER EVER EVER with innoculation of jars. So this guy takes a lil drop of eq. spore water on a microscope slide puts it under the scope and sure enough there's spores. So what the hell happened?! This fellow is not a happy camper and certainly wont be if he doesn't have shrooms to eat when he goes camping! Should he dump the jars or what? oh yes and here's somthing else that is really funny! HE USED DISTILLED WATER TOO! So what up?


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-SumGuy :cool:

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Re: 18 Jars no colonization WHAT GIVES [Re: SumGuy]
    #434043 -

Sounds like a mess. I've never used rye flour on its own, so I can't really comment on that. Did you remember to add water to the substrate before sterilization?

Also, the spores could be neutralized, either by age, heat, or H202. There are a lot of factors here. Retrace your steps and I'll bet you'll find something wrong, especially if you've never had problems before.

You don't list what size jars you used, what was in the substrate, water content, etc. Are you trying bulk?


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"If you believe in things you do not understand, you will suffer." ? Stevie Wonder

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Re: 18 Jars no colonization WHAT GIVES [Re: Insomnia]
    #434055 -

it was rye grain (used many times before), pint jars, and yea obviously the guy added water :P well the only thing i find weird is that not even the agar which already had mycellium grown on it didn't work....The guy thinks that maybe when he sprayed lysol in the jars he didn't wipe them down enough and there was still residue perhaps.... anyhow either way it's a big mess I think he should dump the jars, wash well, and start over!


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-SumGuy :cool:

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Post deleted by users_request [Re: SumGuy]
    #434065 -

Post deleted by administrator.

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Re: 18 Jars no colonization WHAT GIVES [Re: MicronMagick]
    #434077 -

I hate it when that happens. I recently bought and used some hydrated lime that was pure poison to mycelia. I had to toss two dozen jars worth of casings.

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Re: 18 Jars no colonization WHAT GIVES [Re: SumGuy]
    #434109 -

Sounds like bacterial contamination.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: 18 Jars no colonization WHAT GIVES [Re: Anno]
    #434124 -

think he should just throw the jars out all together anno?


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-SumGuy :cool:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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