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Amazon Shop for: ½ Pint Jars, Agar, Microscope, Rye Grain

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InvisibleSumGuy
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Registered: 07/26/01
Posts: 556
18 Jars no colonization WHAT GIVES
    #433972 - 10/23/01 09:33 AM (15 years, 1 month ago)

Ok, my friend's friend's friend pc'd 18 rye jars, two days later innoculated with eq's spore water. 5 days later still no sign of colonization. The next day he decides to take 8 of them and use an agar wedge on each. Then on day 7 he takes 4 more and uses a tapalpa syringe on those 4. So there's 6 With the EQ juice still sitting there, 8 with eq juice and an agar wedge, 4 with tapalpa and eq juice. It's now day 10 and here's the deal, NO growth on the EQ jars, the agar wedges stopped growing, and no sign of the tapalpa growth(mind you it's only been 3 days since innoculation with that. This guy has never had a problem EVER EVER EVER with innoculation of jars. So this guy takes a lil drop of eq. spore water on a microscope slide puts it under the scope and sure enough there's spores. So what the hell happened?! This fellow is not a happy camper and certainly wont be if he doesn't have shrooms to eat when he goes camping! Should he dump the jars or what? oh yes and here's somthing else that is really funny! HE USED DISTILLED WATER TOO! So what up?


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InvisibleInsomnia
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Registered: 07/18/00
Posts: 345
Re: 18 Jars no colonization WHAT GIVES [Re: SumGuy]
    #434043 - 10/23/01 12:08 PM (15 years, 1 month ago)

Sounds like a mess. I've never used rye flour on its own, so I can't really comment on that. Did you remember to add water to the substrate before sterilization?

Also, the spores could be neutralized, either by age, heat, or H202. There are a lot of factors here. Retrace your steps and I'll bet you'll find something wrong, especially if you've never had problems before.

You don't list what size jars you used, what was in the substrate, water content, etc. Are you trying bulk?


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"If you believe in things you do not understand, you will suffer." ? Stevie Wonder


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InvisibleSumGuy
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Registered: 07/26/01
Posts: 556
Re: 18 Jars no colonization WHAT GIVES [Re: Insomnia]
    #434055 - 10/23/01 12:21 PM (15 years, 1 month ago)

it was rye grain (used many times before), pint jars, and yea obviously the guy added water :P well the only thing i find weird is that not even the agar which already had mycellium grown on it didn't work....The guy thinks that maybe when he sprayed lysol in the jars he didn't wipe them down enough and there was still residue perhaps.... anyhow either way it's a big mess I think he should dump the jars, wash well, and start over!


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-SumGuy :cool:


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InvisibleMicronMagick
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Registered: 10/17/01
Posts: 1,026
Post deleted by users_request [Re: SumGuy]
    #434065 - 10/23/01 12:29 PM (15 years, 1 month ago)

Post deleted by administrator.


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Invisiblepuscle
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Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: 18 Jars no colonization WHAT GIVES [Re: MicronMagick]
    #434077 - 10/23/01 12:43 PM (15 years, 1 month ago)

I hate it when that happens. I recently bought and used some hydrated lime that was pure poison to mycelia. I had to toss two dozen jars worth of casings.


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OfflineAnnoA
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Re: 18 Jars no colonization WHAT GIVES [Re: SumGuy]
    #434109 - 10/23/01 01:22 PM (15 years, 1 month ago)

Sounds like bacterial contamination.


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InvisibleSumGuy
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Registered: 07/26/01
Posts: 556
Re: 18 Jars no colonization WHAT GIVES [Re: Anno]
    #434124 - 10/23/01 01:35 PM (15 years, 1 month ago)

think he should just throw the jars out all together anno?


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Amazon Shop for: ½ Pint Jars, Agar, Microscope, Rye Grain

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