Home | Community | Message Board


Edabea
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Aquarium Heater, Brown Rice Flour, Perlite, Rye Grain, Wild Bird Seed

Jump to first unread post. Pages: 1
Offlinekid_alamo
Stranger

Registered: 03/23/02
Posts: 21
Last seen: 10 years, 6 months
Why No Quart Jar Colonization?
    #955826 - 10/12/02 11:10 PM (14 years, 2 months ago)

A friend tried the following tek. Washed, rinsed and steeped finch seed. Added raw sunflower seeds and flaw flax seed then loaded eight quart jars, each with a single micron filter and band, no lid, then tightly covered with aluminum and pressured cooked as usual. Inoculated jars using the oven method and placed in aquarium heater-controlled incubation chamber with some perlite in a small tray. Three days later mycelium appeared on top as expected. Shook jars and returned them to the chamber. Since then no more growth has occurred. No contamination and sadly no evidence of further colonization. If you can’t tell from the pictures the jar content is more wet than dry. What is my friend doing wrong? The incubation chamber did produce a great deal of moisture.  Should he have used the inverted jar lids in addition to the filters? He’s read in some teks where they instruct you to remove the jars immediately from the pc, shake jars and return to the pc to cool. Was leaving the jars in the pc overnight to cool as he did a mistake or should he have included the previously mentioned pc step? Additionally, the jars content breaks apart easily but the core of it appears to have shrunk, darkened and solidified. There is no unusual odor.  Any suggestions? Bluhoney, I would appreciate your imput?  :confused:





 
Quote:

 


 
Quote:

 


 
Quote:

 


 


--------------------
Do not weep; do not wax indignant. Understand. --Baruch Spinoza


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #955857 - 10/12/02 11:35 PM (14 years, 2 months ago)

Looks like wetspot (bacillus) to me.
Why did you add raw sunflower seeds? They are 50% fat, that is bad.
You need a lid with a small hole in it on top of the filter, ie. 3/8", the whole top of the jar is too much area.
I have never used birdseed, but when I use rye I simmer it, rinse it thoroughly, let it drain for half an hour, then PC it and the water content comes out fine. Maybe you just left it too wet.


Edited by zeta (10/12/02 11:36 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekid_alamo
Stranger

Registered: 03/23/02
Posts: 21
Last seen: 10 years, 6 months
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #955873 - 10/12/02 11:47 PM (14 years, 2 months ago)

I found the recipe at the end of a long thread. The explanation for the ingredients were based on a nutritional breakdown of those ingredients including the raw sunflower seeds. Their theory seemed sound. I could try and find it if you'd like. It was a great read.

So the content of the quart jars was to wet? How do I reduce that? If the lids are used is there a need for the incubation chamber. Does the little hole allow enough gas exchnage?


--------------------
Do not weep; do not wax indignant. Understand. --Baruch Spinoza


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekid_alamo
Stranger

Registered: 03/23/02
Posts: 21
Last seen: 10 years, 6 months
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #955880 - 10/12/02 11:52 PM (14 years, 2 months ago)

Now that I think about it, my friend didn't drain the finch seed for a half hour. He just rinsed it through a strainer, shook the excessive water off and added the other ingredients. Maybe the half hour draining makes a difference?


--------------------
Do not weep; do not wax indignant. Understand. --Baruch Spinoza


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblesolidstate
journeyman
Registered: 04/09/00
Posts: 56
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #955932 - 10/13/02 12:24 AM (14 years, 2 months ago)

that stuff is bacillis subtilis, aka wet spot bacteria.
wet spot occurs when the grain jars are not sterilized long enough in the pc, can also be airborne & enter jars during innoculation. the endospores naturally occur on most grains & are somewhat heat resistant, hence the importance of proper sterilaztion of grain jars.
I suspect your probem is not so much your recipe as your sterilization tek.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekid_alamo
Stranger

Registered: 03/23/02
Posts: 21
Last seen: 10 years, 6 months
Re: Why No Quart Jar Colonization? [Re: solidstate]
    #955944 - 10/13/02 12:38 AM (14 years, 2 months ago)

I think I will soak the seed for 24 hours before steeping. I read it brings most of the endospores out for better sterilazation.


--------------------
Do not weep; do not wax indignant. Understand. --Baruch Spinoza


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: kid_alamo]
    #956017 - 10/13/02 01:20 AM (14 years, 2 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekid_alamo
Stranger

Registered: 03/23/02
Posts: 21
Last seen: 10 years, 6 months
Re: Why No Quart Jar Colonization? [Re: matts]
    #956041 - 10/13/02 01:35 AM (14 years, 2 months ago)

Starbucks just called, they want my recipe!


--------------------
Do not weep; do not wax indignant. Understand. --Baruch Spinoza


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 14 years, 24 days
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #956195 - 10/13/02 03:18 AM (14 years, 2 months ago)

try the BRF tek instead, it works for me and is much easier. you only need 1/2 pint jars for that


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegoldtop
Wild Spore

Registered: 07/28/01
Posts: 115
Loc: Sydney, Australia
Last seen: 11 years, 5 months
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #956208 - 10/13/02 03:27 AM (14 years, 2 months ago)

Kid_alamo

I used to have exactly the same problem as you. No matter what I tried, my jars kept getting a wet bacterial contamination...sometimes even when the jars where almost 90% colonised, but mostly earlier. My jars looked exactly like the picture of your jars.

I tried lots of things - thoroughly washing and rinsing the grain, preboiling the grain, trying grain from a different supplier, Pressure cooking at a higher temp, pressure cooking longer. I even tried combinations of these. None of these things worked. I'm not saying they won't work for you. All I'm saying is that they didn't work me.

The only way I could control contamination was by pressure cooking twice. I would pressure pressure cook the grain once, then take the jars out of the PC, shake them, and leave alone for 24hours. I would then pressure cook them again.

Problem fixed. I've PC over 100 jars using this method, with zero contamination.

I know pressure cooking twice is annoying, and very time consuming, but it certainly worked for me. I'm not sure why pressure cooking twice (with a 24 hour wait in between) works, all I know is that it does work.


--------------------
"I want it all, I want it all, I want it all, and I want it NOW!" - Queen


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekid_alamo
Stranger

Registered: 03/23/02
Posts: 21
Last seen: 10 years, 6 months
Re: Why No Quart Jar Colonization? [Re: goldtop]
    #956648 - 10/13/02 11:31 AM (14 years, 2 months ago)

Goldtop

Very interesting. Were you using finch seed? I'm willing to try the double pc treatment. I do have some questions. You wrote, "I would pressure pressure cook the grain once, then take the jars out of the PC, shake them, and leave alone for 24hours. I would then pressure cook them again."

Are you taking the jars out while they are hot? If so I assume you first remove the pressure controller and let all the steam escape before opening the pc and shaking the jars? Do you return the jars to the pc for the 24 hour period or can I place them on a shelf and continue to sterilize other jars? I've read a similar step in a quart jar tek but chose to let mine sit undisturbed in the pc until they cooled naturally.

I appreciate you sharing your experience with this particular problem. Your results make me hopeful again. :cool:

What type of lid treatment did you employ?

Peace 


--------------------
Do not weep; do not wax indignant. Understand. --Baruch Spinoza


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegoldtop
Wild Spore

Registered: 07/28/01
Posts: 115
Loc: Sydney, Australia
Last seen: 11 years, 5 months
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #958113 - 10/13/02 11:03 PM (14 years, 2 months ago)

Make sure theres no pressure in the PC before you open it.

Then, using a clean cloth so you don't burn your hands, grab the jar, shake it, then put it somewhere clean. I put them in a closed cardboard box that i've sprayed lighly with 10% bleach solution. Anywhere clean is ok. An open shelf might be a litle dusty, but a glass covered shelf would be better. Just try to keep them as clean as you can.

You can steralize other jars while the first lot are waiting.

I buy finch seed from the local pet shop, which is mostly millet. The store owner

For the lid, I've drilled 2 small holes, and I've plugged them polyester polyfill. I then cover the top 1/3 of the jar once with aluminium foil. I cover it with a 2nd layer of aluminium foil that cover the top 1/2 of the jars. There might be other ways of doing it, but thats how I do it and it works well.


--------------------
"I want it all, I want it all, I want it all, and I want it NOW!" - Queen


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekid_alamo
Stranger

Registered: 03/23/02
Posts: 21
Last seen: 10 years, 6 months
Re: Why No Quart Jar Colonization? [Re: goldtop]
    #958379 - 10/14/02 12:25 AM (14 years, 2 months ago)

Clarify some finer points for me. After you pc your jars the first time do you leave both layers of foil on while waiting for the second round? I'm assuming so.

After the second round when you move the jars to an incubation chamber do you leave the foil on or remove it? If left on, do you loosen it at all? What about the polyfill at this stage while in the chamber. Remove it or leave in place?

I'm using plastic lids and plan on trying the polyfill technique. What do you think of using micron filters in conjunction with the polyfill lids/foil?



--------------------
Do not weep; do not wax indignant. Understand. --Baruch Spinoza


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehappygrins
addict

Registered: 12/30/01
Posts: 588
Loc: Shroomery
Last seen: 11 years, 9 months
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #959280 - 10/14/02 10:02 AM (14 years, 1 month ago)

After you have finished PCing them and there is no pressure in the PC, open it up.  Grap a towel to take them out.  Tighten the lids. Shake really well.  Take off tin foil. Leave polyfill on lid.  After they have cooled 100% (atleast 12 hours), innoculate with syringe through the polyfill as close to the edge of polyfill filter and the opening you made in the jar lid. Do not innoculate through the center of the polyfill filter as this will push the whole filter into the jar. Shake jars again and place in incubator.

--happygrins :grin:


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Why No Quart Jar Colonization? [Re: kid_alamo]
    #959396 - 10/14/02 12:08 PM (14 years, 1 month ago)

I am no pro ......... but I bet you will continue to have troubles as long as you keep using sunflower seeds . Sun flower seeds do not hold up well in high moisture environments , they like to soak up lots of water then rupture when heated .
This is just my hunch !
Good Luck .
And yes work on that sterilization process .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Aquarium Heater, Brown Rice Flour, Perlite, Rye Grain, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Quart jar colonization - when complete? farmer_maggot 1,838 1 09/12/01 04:48 AM
by NDK
* 1 Quart jars colonizing time?
( 1 2 all )
v1ru5 4,177 20 03/15/07 03:42 PM
by RogerRabbit
* 71 Quart Jars Colonized. Now what? (update w/ mature mushroom pics)
( 1 2 3 4 5 6 7 8 9 all )
FurrowedBrow 13,460 162 12/21/07 02:12 PM
by RogerRabbit
* 10cc puerto rican&10cc EQ 7-10 quart jars first time? CCBIGGS 283 1 01/31/09 08:39 PM
by CCBIGGS
* quart jars not colonizing Chainer 905 18 03/15/09 04:01 PM
by Shroominit
* Quart Jar Question -- Polyfill MOTH 884 10 01/25/05 10:21 PM
by Baby_Hitler
* Quart Jar Suprise YellowSubmarine 1,000 5 12/02/02 04:28 AM
by YellowSubmarine
* Good Quart Jar Teks Anonymous 1,317 4 01/20/03 05:24 AM
by SixTango

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
1,991 topic views. 14 members, 68 guests and 26 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Lil Shop Of Spores
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.048 seconds spending 0.002 seconds on 14 queries.