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Amazon Shop: ½ Pint Jars, Aquarium Heater, Brown Rice Flour, Vermiculite

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Registered: 11/18/02
Posts: 457
Last seen: 12 years, 1 month
Jar Colonization
    #1216839 - 01/14/03 07:53 AM (15 years, 3 months ago)

I inoculated 7 jars with ecuador spore solution on the 19th of December. I then left my apartment to go home for winter break, and came back on Jan 10 to jars that were from 75%-95% colonized. I had left the heat at about 70 F (don't have to pay for it, but although I could have left it on higher, I didn't want to piss anyone off), and upon my return I bought a 73 qt Sterlite container and put my aquarium heater to use in a pitcher. After 3 days at 82-86 F (my roommates keep changing the heat and fucking up the temps inside the incubator), I have determined 4 jars to be 100% finished. The other three, however, are not quite finished. The weird thing is, the bottom is finished on all three. I was under the impression that the bottom is the last thing to be colonized, but this doesn't seem to be the case here. Two of the jars are kind of weird looking (compared to the 100% jars). They seem to have some areas of rhizomorphic growth, but in these spots the BRF/verm seems to be showing through. This could be normal, but as this is my first time, I don't know. Each of these jars also seem to have "low spots" around the top of the jars; I can't tell if this is because some of the dry very layer dropped down or not. It doesn't look like the uncolonized portion is the BRF/verm mix; rather it lookes like just verm. Anyway, I'd guess that each jar is 90-95% colonized. I don't think it'd do too much good to flip them, since the bottom is already finished. The third jar is 85-90% colonized; the problem with this one is that I ran out of spore solution on the last hole of the last jar. This one too, however, is fully colonized on the bottom.

I don't know, I guess I'll just put them back in the incubator for another few days and hope for the best. I wish I could provide pics, but I don't have a camera. I know the "low spots" must seem vague . . . the best way I can describe it is as follows: 100% jars have a level of colonization around the top of the jar that is even all the way around. These jars have spots where colonization drops to lower levels of height, and then picks back up. The weird part is that around these low spots, the growth is distinctly rhizomorphic.

Anyway, sorry this is so long, if you've read down to here I appreciate it.
Any suggestions on how to proceed would be more more than welcome. Thanks a lot.

Edit -- So to recap, I have 4 jars that are 100% done; I have 3 that aren't, and they have problems as described above.


Edited by shroominbloom (01/14/03 07:59 AM)

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Registered: 06/12/02
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Re: Jar Colonization [Re: shroominbloom]
    #1216857 - 01/14/03 07:57 AM (15 years, 3 months ago)

Let the ones with 70%-90% colonization colonize without the incubator if it will cause problems. Take the other ones with the growth primarily at the bottom and flip them over and then let them colonize in the incubator.

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i have arrived

Registered: 11/18/02
Posts: 457
Last seen: 12 years, 1 month
Re: Jar Colonization [Re: SixCee]
    #1216893 - 01/14/03 08:04 AM (15 years, 3 months ago)

Alright I flipped them; I'll just leave them for 3 days or so and then check again . . . hopefully they'll be done.


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Amazon Shop: ½ Pint Jars, Aquarium Heater, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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