Home | Community | Message Board


Everything Mushrooms
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars

Jump to first unread post. Pages: 1
OfflineNITRIC
journeyman
Registered: 02/24/01
Posts: 61
Loc: the buckeye
Last seen: 12 years, 8 months
H. Peroxide in spore water
    #424733 - 10/14/01 02:29 AM (15 years, 1 month ago)

heres the thing,
Lets say you wanted to mass produce " spore water"
for example you take 1 pint jar fill to the top line w/ distilled water and you pressure cook for 1 hour then let cool, then put the jar into a sterial enviroment w/ a screen on top of the jar and put a cap on top of the screen, then in a day or so the spores drop in the water, then you pull off the cap and screen and put the lid on and presto you have spore water.
my question is if you put H. peroxide in w/ the water would it affect the spores? later?
lets say a 20 :1 ratio or less or more?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesylo
addict
Registered: 10/30/00
Posts: 219
Last seen: 14 years, 7 months
Re: H. Peroxide in spore water [Re: NITRIC]
    #424780 - 10/14/01 03:56 AM (15 years, 1 month ago)

Peroxide kills spores. It is only safe to use with mycelium.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNITRIC
journeyman
Registered: 02/24/01
Posts: 61
Loc: the buckeye
Last seen: 12 years, 8 months
Re: H. Peroxide in spore water [Re: sylo]
    #424839 - 10/14/01 06:28 AM (15 years, 1 month ago)

how do you know this?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 15 days
Re: H. Peroxide in spore water [Re: NITRIC]
    #424925 - 10/14/01 10:29 AM (15 years, 1 month ago)

>how do you know this?
Perhaps he visited www.mycomasters.com and read their Manuals.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTamrylin
member
Registered: 08/18/01
Posts: 170
Loc: USA, East Coast
Last seen: 14 years, 4 months
Re: H. Peroxide in spore water [Re: NITRIC]
    #425545 - 10/14/01 09:12 PM (15 years, 1 month ago)

actually yeah, h202 does massacre spores.. if you could concentrate the liquid to millions of spores per .. uh.. really small space, you could probably add a couple drops h202, but it is sort of pointless. Why not just use honeywater w/o h202 for your spores, let it become a bit of mycelium, then add the h202? you could make mycelium syringes instead..


--------------------
Tangerine trees and marmalade skies...


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinejonnyshaggs420
Pooh-Bah

Registered: 08/09/00
Posts: 1,965
Loc: Mid-West
Last seen: 11 years, 2 months
Re: H. Peroxide in spore water [Re: NITRIC]
    #425749 - 10/14/01 11:57 PM (15 years, 1 month ago)

Peroxide is a deadly toxin. It is broken down by peroxidases in the body, namely catalase. Spores contain no peroxidases, and they die, bacteria also contain no peroxidases and they die, but mycelium does produce peroxidases so it does not die.


--------------------
Vote Jonnyshaggs in the next election for GOD...Its the responsible choice


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineYakul
Listener
Female User Gallery

Registered: 09/15/14
Posts: 44
Last seen: 1 year, 10 months
Re: H. Peroxide in spore water [Re: NITRIC]
    #20569074 - 09/15/14 01:26 AM (2 years, 2 months ago)

I've been thinking about trying a method like this because spore printing and then scraping that print into water seems a bit labor intensive. This method, as you described, with the spore-straight-into-water aspect, sounds good.  But my question is, how long can you store that spore water for before it can no longer be used?  Can it be frozen?  Thanks.


--------------------
"Only an idiot is entirely certain about everything all the time."-Sadguru


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineYakul
Listener
Female User Gallery

Registered: 09/15/14
Posts: 44
Last seen: 1 year, 10 months
Re: H. Peroxide in spore water [Re: Tamrylin]
    #20569079 - 09/15/14 01:27 AM (2 years, 2 months ago)

what is honeywater?


--------------------
"Only an idiot is entirely certain about everything all the time."-Sadguru


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineYakul
Listener
Female User Gallery

Registered: 09/15/14
Posts: 44
Last seen: 1 year, 10 months
Re: H. Peroxide in spore water [Re: Tamrylin]
    #20569134 - 09/15/14 01:53 AM (2 years, 2 months ago)

Have you tried this?  Did it work?


--------------------
"Only an idiot is entirely certain about everything all the time."-Sadguru


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePussyFartM
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 21,827
Loc: Orbiting Earth
Last seen: 34 minutes, 21 seconds
Trusted Cultivator
Re: H. Peroxide in spore water [Re: Yakul]
    #20569712 - 09/15/14 09:01 AM (2 years, 2 months ago)

Quote:

Yakul said:
I've been thinking about trying a method like this because spore printing and then scraping that print into water seems a bit labor intensive.



Really? This is not the hobby for you....


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
  ^*~.,,.~*^*~.,_,.~*^*~.,_SHIT FOR NOOBS_,.~*^*~.,_,.~*^*~.,_,.~* 


Post Extras: Print Post  Remind Me! Notify Moderator
OnlineBuster_Brown
uninitiated
Male User Gallery

Registered: 09/17/11
Posts: 3,818
Last seen: 39 seconds
Re: H. Peroxide in spore water [Re: Yakul]
    #20569718 - 09/15/14 09:06 AM (2 years, 2 months ago)

Quote:

Yakul said:
what is honeywater?




LC


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKizzle
Misanthrope

Registered: 08/30/11
Posts: 8,642
Last seen: 1 day, 3 hours
Re: H. Peroxide in spore water [Re: Buster_Brown]
    #20580629 - 09/17/14 07:53 PM (2 years, 2 months ago)

Quote:

I've been thinking about trying a method like this because spore printing and then scraping that print into water seems a bit labor intensive.



Yeah sometimes I have to stop and catch my breath and my arms are just sore for days from all that heavy lifting:lol:

Seriously though I can tell you peroxide isn't going kill all that bacteria falling off the cap into the water anyway. What does kill the bacteria is making a spore print, the bacteria dry out and die.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinetchyted
miestro
Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 2 years, 2 months
Re: H. Peroxide in spore water [Re: NITRIC]
    #20597675 - 09/21/14 06:05 PM (2 years, 2 months ago)

i think what you suggest has been done, wrong, a lot!

i think in my research, there was a published tek similar to what you want, that was published by the original PF.  that tek, omits the water and the peroxide, but is otherwise much the same.  it was done inside half-pint jars.  i suspect that this is the tek that PF used in collecting the spores he used to sell.

after the spore-print is made, it is turned into bulk spore-water which in turn got diluted before sale.  this should provide you with a copious supply of clean spore water if properly adapted.

when i tried it, i was just a noob, so i ended up with nothing but contams and abandoned that procedure.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineYakul
Listener
Female User Gallery

Registered: 09/15/14
Posts: 44
Last seen: 1 year, 10 months
Re: H. Peroxide in spore water [Re: Kizzle]
    #20662213 - 10/05/14 06:01 PM (2 years, 2 months ago)

Thanks for giving a real reply that isn't rude and actually quite helpful!


Edited by Yakul (10/05/14 06:05 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineYakul
Listener
Female User Gallery

Registered: 09/15/14
Posts: 44
Last seen: 1 year, 10 months
Re: H. Peroxide in spore water [Re: PussyFart]
    #20662223 - 10/05/14 06:03 PM (2 years, 2 months ago)

Yeah, because if something can be done much easier, why not do it the hard way?  Right Pussyfart?" Forget what Henry Ford did to all future industry by looking for more efficient ways to work.  And forget innovation, Pussyfart!  You're right!  Why push the horizon? It's not like you can make new discoveries or ways of saving time in this age of science and information!  What a great name, by the way, Pussyfart.  I love it!  Thank you for taking the time to reply with such helpful information and life advice.  I'm sure you must be very busy with your "hobby," but you managed to find time in your day to insult a stranger.  How kind of you.  You are a genuinely mature adult, of which there are far too few in this world. 
And now, predictably, under the disguise of the internet, we will watch as Pussyfart puts his foot where his mouth ought to be in his inevitably assinine reply to this.  :-)


Edited by Yakul (10/05/14 06:22 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePussyFartM
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 21,827
Loc: Orbiting Earth
Last seen: 34 minutes, 21 seconds
Trusted Cultivator
Re: H. Peroxide in spore water [Re: Yakul]
    #20662953 - 10/05/14 08:25 PM (2 years, 2 months ago)

:lmafo:


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
  ^*~.,,.~*^*~.,_,.~*^*~.,_SHIT FOR NOOBS_,.~*^*~.,_,.~*^*~.,_,.~* 


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineweaksause
Stranger

Registered: 08/26/14
Posts: 254
Last seen: 1 year, 10 months
Re: H. Peroxide in spore water [Re: Yakul]
    #20675398 - 10/08/14 03:19 PM (2 years, 2 months ago)

Quote:

Yakul said:
Yeah, because if something can be done much easier, why not do it the hard way?  Right Pussyfart?" Forget what Henry Ford did to all future industry by looking for more efficient ways to work.  And forget innovation, Pussyfart!  You're right!  Why push the horizon? It's not like you can make new discoveries or ways of saving time in this age of science and information!  What a great name, by the way, Pussyfart.  I love it!  Thank you for taking the time to reply with such helpful information and life advice.  I'm sure you must be very busy with your "hobby," but you managed to find time in your day to insult a stranger.  How kind of you.  You are a genuinely mature adult, of which there are far too few in this world. 
And now, predictably, under the disguise of the internet, we will watch as Pussyfart puts his foot where his mouth ought to be in his inevitably assinine reply to this.  :-)




aight, that's kinda unwarranted. i mean he registered a few
years ago, is a TC, and has 18k+posts . i'd rephrase that
with a little respect , and sincerely ask the dude why
his name is pussyfart, hahaa good riddance man,christ

side note , in real life who the fuck can stand u
besides your poor mother, god bless her

Quote:

Really? This is not the hobby for you....




appropriate response to someone in adv. mycology
stating spore printing and scraping too much labor

and what i was going to post
:::
why not print into a dry sterilized mason jar
then fill with water? what's the point of the spores
going directly into water? thats the method i used
and plan to continue using ...

/e
p.s. he never insulted you. you're insulting him
in a very immature and annoying way.
p.p.s if you think putting a cap ontop of peroxidated(Sp..)
h2o is going to be an innovating ... uh ? quick google
search reveals peroxide kills spores, and putting a cap
ontop of water makes no sense (to this noob) when u can
put it ontop of glass then fill taht glass w. water

lol:o aight putting down the crack pipe in sparkin this
blunt with my dog ... got worked up haha


Edited by weaksause (10/08/14 03:23 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* peroxide help - by mycofile Raadt 1,417 2 12/13/02 08:53 AM
by Azmodeus
* Does H2O2 KILL spores or just inhibit germination?
( 1 2 all )
micro 4,324 37 11/26/03 11:24 AM
by debianlinux
* Hydrogen peroxide agar upside down TEK, interesting little technique ranonar 4,329 17 08/11/13 05:40 AM
by fastfred
* peroxide dunk before tissue transfer? MotorCityMadman 2,369 8 08/09/07 04:00 AM
by jeanpignon
* Peroxidated grains MickyWilliams 1,890 13 01/18/06 12:27 PM
by mycofile
* What is an effective use of Hydrogen Peroxide?
( 1 2 all )
garret 10,889 38 05/26/08 04:36 AM
by Tequila
* Single Strain (spore) Isolation and the Benefits.. TrippinRhino 4,818 18 07/24/01 02:02 AM
by Anno
* Do you use peroxide before G2G Gr0wer 1,371 12 08/03/04 10:43 AM
by stargazer

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
2,001 topic views. 0 members, 0 guests and 4 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Kraken Kratom
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.073 seconds spending 0.004 seconds on 14 queries.