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Anonymous

Re: recovery after peroxide exposure
    #86166 - 06/08/00 03:08 PM (24 years, 14 days ago)

thanks for the insight, Moe, it helps to hear the gut feeling sometimes, you know? I mean ideas and observations and facts are cool and all, but gut feeling has steered my mycelial investigations the right way more than once.

"If you have unestablished fungus, you can actually taint the substrate if you go getting silly with the H2O2 slashig it all ove the place getting all fancy crazy with it."

well Id like to know others opinions on this matter. After all, if moes hunch is right, then a lot of teks on the grow/find section contain misinformation. I mean, in the sterile tissue cloning procedure it says to
take colonized fungus, dip it in 3% peroxide and 'fizz around for a while'. thats pretty fuzzy instructions to be in a tek. i cant remember said it, and i cant find the thread in a search, but someone once posted the correct concentrations of peroxide for various uses.


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InvisibleRipper
enthusiast
Registered: 01/19/00
Posts: 223
Re: recovery after peroxide exposure
    #86169 - 06/08/00 03:36 PM (24 years, 14 days ago)

Well here's Ripper's two cents on the ordeal. Think about this, Mycellia contains DNA, which is a VERY fragile thing. Peroxide may or may not affect the DNA structure of mycellia, no one really knows for certain. Most commercial mushroom farms have no interest in the use of it because it doesn't fly with the organic commision. So no conclusive studies that have been published can say one way or the other. But to give you an example as to how delicate Stamets feels the fungal DNA may be, Stamets recommended that we carry the cultures we were given on our persons and not place through an x-ray machine. So when I think of whether or not applying peroxide to a piece of mycellia that in turn is going to be spawned into large masses, I really must say "no".

Ripper


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Anonymous

Re: recovery after peroxide exposure
    #86170 - 06/08/00 03:49 PM (24 years, 14 days ago)

fascinating guys, and you know what? my friend says she did it with straight 3% peroxide and shook it up and let it sit for 10 minutes. She also did another piece in a jar with distillied h20 and only 2 cc's of 3% peroxide, also shook and let sit for 10 minutes. Both mycelium looked pretty messed up afterwards, like white scum floating around fizzing.

She then innoculated. Let you know what happens. Oh, BTW there was some left over slurry and she put it in the fridge to store it and test its longevity, if its still alive.


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Anonymous

Re: recovery after peroxide exposure
    #86171 - 06/08/00 04:04 PM (24 years, 14 days ago)

quote:
Originally posted by Moe Howard:
Alright. Peroxide is effective in keeping most molds from DEVELOPING.

Replace DEVELOPING with germinating and you will be spot on... Already germinated mold spores will not be kept at bay by those levels of H2O2. Same with mushroom and other fungi. Spores will be neutralized by mycelium can grow through it.

The Learner


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Invisiblemycofile
Pooh-Bah
 User Gallery

Registered: 01/18/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: recovery after peroxide exposure
    #86172 - 06/09/00 07:11 AM (24 years, 13 days ago)

why is nobody using peroxide agar? again I refer to the excellent write up by placebo months ago, use the search function. again i say it is amazing. and also likely again, nobody listens to me.

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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