Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Avalon Magic Plants
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
How to mix Peroxidated MEA
    #582784 -

WHat is the best way to mix up Peroxide in cooled MEA?  I can't seem to get even distribution... what do the pros use?

:smile:

thx in advance

Extras: Filter Print Post Top
Re: How to mix Peroxidated MEA [Re: Richard_D_James]
    #582878 -

I don't know what pros do, but here's what I do:
First, I mix up agar, malt, potato dextrose and melt the solution on the stove. I then place the mixture in a 500 ml flask, seal the top with a double layer of tinfoil, and pressure cook at 15 psi for 10 minutes. I allow the cooker to release all the pressure, and when the mixture is still hot, I take it out of the cooker. When the flask is cool enough to touch without burning your fingers, I add 7% H2O2(which is a 3% concentration H2O2 when it comes in the bottle) to the liquid malt and swirl the flask until the peroxide is evenly distributed.
If I use my pyrex petri dishes, I sterilize the clean, empty dishes with the flask. If I use plastic petri dishes, I merely open the package and use them immediately because they come sterile. I pour the dishes on an anal retentively sterilized surface(or at least pretty close to sterilized) and allow them to solidify. Once they are solid, I use the oven and transfer sterile tissue to the new plates, and then seal the plates with parafilm. Saran wrap works also if you don't have such luxuries.
When using 10%, I noticed the tissue samples did not like it too much and stalled for weeks. With a 7% concentration, contams can still occur if you're not careful, but at least the majority are killed and the tissue adapts rapidly to the medium.

Extras: Filter Print Post Top
Re: How to mix Peroxidated MEA [Re: Azure]
    #582898 -

7% concentration? and this works??

Wayne says to use no more than 5 ml of the 3% peroxide per 500 ml of agar.


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

Extras: Filter Print Post Top
Re: How to mix Peroxidated MEA [Re: Richard_D_James]
    #583080 -

There was a thread in this forum about this SAME issue this last week. Search for it....

Peace,
MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

Extras: Filter Print Post Top
Re: How to mix Peroxidated MEA [Re: Azure]
    #583086 -

right....rush says 6-8ml / liter water, broth whatever...so 3.5 ml / 500 ml agar mix...is your .7 %///
used this in my liquid culture and mycelia floated to top where it was easy to
syringe out through the hole.......but now the remaining mycelia culture, 24 hrs later,
has matted up ....pretty solid...expect some recovery time, but....
any experience with this.??..I/l post as a question...later...I guess...farmer

Edited by karlfinn (03/19/02 03:07 PM)

Extras: Filter Print Post Top
Re: How to mix Peroxidated MEA [Re: Anno]
    #583443 -

It definitely works, but the reason it does is probably because the solution is still relatively hot when I add the peroxide to it, which breaks down the the peroxide and lowers the molarity.

Extras: Filter Print Post Top
Re: How to mix Peroxidated MEA [Re: Azure]
    #583488 -

azure....is that 7 % or . [point] 7 % ?... one is 7 ml pox / liter , ...or...other;... 70 ml pox / liter..???
all real new to me but I just the lesser amount..??

Extras: Filter Print Post Top
Re: How to mix Peroxidated MEA [Re: karlfinn]
    #591085 -

word. when i was reading earlier someone suggested the use of a candy thermometer, stick it in your agar mix whatever it may be, wait till it is at 140 deg. then pour in the H202, at this temp the agar mix is still liquid, yet cool enough not to break down the H202.


--------------------
___________________________________________________________
"the weekend has landed all that exists now is clubs,drugs, pubs, and parties" - Human Traffic

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Problems getting Rhizo growth from MEA
( 1 2 all )		 upupup 5,623 32 12/05/02 06:44 AM
by stonErollEr1
* Questions about MEA and peroxide... upupup 1,541 4 11/22/02 07:46 PM
by Joshua
* Unsteralized Peroxide Growing puwtrip 3,998 10 05/14/05 10:47 PM
by Snarp
* Preparing MEA petri-dishes for future usage? Arp 767 1 05/29/05 06:55 AM
by ohmatic
* Dr Rush Wayne's Peroxide sterilisation process .. !!! InfiniteAdam 5,461 12 11/17/03 01:03 PM
by mycofile
* Prefered agar mix? them_26 5,656 17 03/27/02 09:41 PM
by Lana
* Need suggestions about peroxidated agar CLuB99 1,546 4 08/11/01 04:18 PM
by Anno
* recipe for peroxide?
( 1 2 all )		 sleepingbigguy 3,770 22 04/19/05 09:18 PM
by Abermelin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta
2,183 topic views. 0 members, 3 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.007 seconds on 16 queries.