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Amazon Shop for: ½ Pint Jars, Petri Dish, Rye Grain

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Offlineliquidplaya
member
Registered: 06/08/00
Posts: 124
Last seen: 15 years, 30 days
Liquidplaya's 99% contam free grain -> X TransfTek
    #415559 - 10/06/01 07:22 AM (15 years, 2 months ago)

I just had this vision in my dream about a way to transfer rye to new jars of rye.. I've been using the "lift the lid quickly and hope it doesnt contam" method.. but I just figured out this way to do it with almost no chance of contams.. at least a LOT less than opening the lid..

So.. I bring you
Liquidplaya's 99% contam free grain -> XXXX Transfer Tek"

You need:
- your grain master jar
- your XXX jar. (XXX because you can transfer it to any other type of jar)
-Note: your lids must be the 2-part type with the disk and the screw on surrounding lid.

1. Line up your jars onj your sterile environment.

2. SHake the master jar so that the individual kernels of Rye/seed are separated.

3. Remove the master jar's outside ring while holding in place with one finger the disk so that it does not move. Place the ring to the side...

4. Remove the disk on the jar you are currently transfering the grain/seeds to.

5. **the only slightly tricky part, but if you can play yahtzee then you should be able to do this**
Flip the master jar onto the transfer jar so that the master jar is sitting on top of the transfer jar upside down.

6. *Optional* Place a piece of plastic or similar material around the side of where the jars connect, and hold the jars on that side.

7. Then with the other hand, slide the discs both out a little until the desired amount of master grain falls into the bottom jar.

8. Replace the disks back to their original position.

9. Flip the master jar back over, and replace the newly innoculated jar's ring.

10. Repeat steps 4 - 9 for as many jars as you want.

11. Replace the master jar's lid.

ANd thats it!
let me know what you think..





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Invisiblepuscle
genius of love
Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: Liquidplaya's 99% contam free grain -> X TransfTek [Re: liquidplaya]
    #415562 - 10/06/01 07:30 AM (15 years, 2 months ago)

Nice idea!



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OfflineGrefa
Lab Technician
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Registered: 02/20/15
Posts: 170
Last seen: 8 hours, 5 minutes
Re: Liquidplaya's 99% contam free grain -> X TransfTek *DELETED* [Re: liquidplaya]
    #23769577 - 10/25/16 12:35 PM (1 month, 9 days ago)

Post deleted by Grefa

Reason for deletion: because im fucking stupid.



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OfflineGreg


Registered: 10/29/15
Posts: 1,067
Last seen: 21 minutes, 32 seconds
Re: Liquidplaya's 99% contam free grain -> X TransfTek [Re: Grefa]
    #23769589 - 10/25/16 12:40 PM (1 month, 9 days ago)

Edit: just realized this post is 15 years old :facepalm:
Don't bump old shitty threads please.

Every time you do this grains touch the edges of the disk which is not a sterile surface.
I only get contams about every 30 jars I A2G or G2G the normal ways.


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OfflineMycolorado
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Registered: 07/23/16
Posts: 577
Loc: Choice City
Last seen: 1 hour, 24 minutes
Re: Liquidplaya's 99% contam free grain -> X TransfTek [Re: Greg]
    #23769596 - 10/25/16 12:44 PM (1 month, 9 days ago)

This sounds like a good way TO GET contamination.  You don't want any contact between vessels when making transfers, whether jar, bag, petri, etc...

Whoops...just saw your note on the thread age.


--------------------
"Microscopic delights are chiefly to be summoned to one's aid for the exact identification of fungi"
                                BATSCH, El. Fung. vol. I, p. 19.




Edited by Mycolorado (10/25/16 12:45 PM)


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OfflineGreg


Registered: 10/29/15
Posts: 1,067
Last seen: 21 minutes, 32 seconds
Re: Liquidplaya's 99% contam free grain -> X TransfTek [Re: Mycolorado]
    #23769600 - 10/25/16 12:45 PM (1 month, 9 days ago)

Yeah, definitely a bad idea.


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Invisiblebodhisatta
Fuck
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Registered: 04/30/13
Posts: 24,304
Re: Liquidplaya's 99% contam free grain -> X TransfTek [Re: Greg]
    #23769607 - 10/25/16 12:52 PM (1 month, 9 days ago)

Sounds like a great way to make simple G2G MORE contamination prone.

Nice necrosis bump


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OfflineGreg


Registered: 10/29/15
Posts: 1,067
Last seen: 21 minutes, 32 seconds
Re: Liquidplaya's 99% contam free grain -> X TransfTek [Re: bodhisatta]
    #23769623 - 10/25/16 12:59 PM (1 month, 9 days ago)

The guy who bumped this said he uses a glovebox as well...
Seriously bad juju.


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Amazon Shop for: ½ Pint Jars, Petri Dish, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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