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Growing on bread
    #4131390 -

I have calculated that standard sliced bread should need about 50ml water per 100g added.
This is presuming rye has 10% water content and gets 110ml water per 100g. So you are really adding 110+10ml water to 90g fully dry grain.
Bread is an average of 35% water. So you have 65g dry bread and 35ml water +50ml additional to have approximately the same amount of water.
I was thinking of making microwaved vermiculite sandwiches.

Can anybody comment on the sterilization of bread needed. Seeing as it has already been ground, processed and baked does it need PC sterilization or will steam do.

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Re: Growing on bread [Re: blackout]
    #4131422 -

Use wheat bread. It's a type of grain...I'd Pc IMO...

-Gnostic

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Re: Growing on bread [Re: IGnosticAbhorI]
    #4131430 -

Wheat bread is the cheapest for me, rye is expensive here. Brown would also be best. I was thinking that a whole loaf could be stuck in a autoclave bag with a bit too little water. Then when PC'd I could make up the missing water with liquid innoculant, this would also mean the cooked bread would be drier and less likely to collapse into a soggy mess.
Microwaved bread usually turns into a rock hard lump which could be useful in this case as that hard lump would still be very airy. You could fractionally sterilise it by adding a small amount of water each day so it is neither bone dry nor soggy. I like the idea of dry bread sucking up liquid innoculant like a sponge. The bread could be dipped in other nutrient mixes too.

Edited by blackout (05/04/05 07:46 AM)

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Re: Growing on bread [Re: blackout]
    #4131436 -

Hmm...Rye bread, Didn't even think of that :rolleyes:

Why brown? Wouldn't wheat be second best to rye?

-Gnostic

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Re: Growing on bread [Re: IGnosticAbhorI]
    #4131439 -

Nutrient mixes...

Don't really like the sound of that. Sounds like a contam factory....I dunked my cake in a honey solution (like for lc's)..

Greenie farms R us :smirk:

-Gnostic

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Re: Growing on bread [Re: IGnosticAbhorI]
    #4131440 -

Regular brown bread is wheat bread (in my parts), but just unbleached just like the difference between brown and white rice, it is not as processed so has more nutrients.

Also stale bread may work better as it is already hard, cheaper too, free from some bakeries.

I am thinking nutrient mixes prior to sterilization, like dipping it in manure tea.

Salt content is my only worry but there seems to be less these days since people are more wary of salt in their diets

Edited by blackout (05/04/05 07:52 AM)

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Re: Growing on bread [Re: IGnosticAbhorI]
    #4131445 -

O gotcha

Yeah. Manure Tea.....I don't think anyone ever realized that when you sterilyze the tea (when it's in w/e...grains...bread ect ect) , lots of nutes are destroyed (or was it bacteria)...

Never really was a big fan of stuff like that, but i did it cuz Agar recommened it...lol...

I think dry bread isn't such a bad idea....I have a Shit-load of liuid innoculant...Any recommended ideas for how much of what? :smirk:

-Gnostic

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Re: Growing on bread [Re: IGnosticAbhorI]
    #4131467 -

It is sort of hard to determine how much moisture is in your already dry bread. If you get it fresh and weigh it and then leave it out on racks to dry, or in a low heated oven you can add moisture as I mentioned already, i.e. 50ml per 100g fresh bread. This will be handy if you have no scales, most bread comes in 800g pans where I am. You could even just lighlty toast the bread to harden it more.

I am not a fan of manure either, but some sort of additional nutrients could make it a very good substrate, its airiness could leave it acting like vermiculite itself.

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Re: Growing on bread [Re: blackout]
    #4136264 -

I jammed a 100g single piece of white bread into a jar and poured on some weak coffee. Put a filter disc on the top and microwaved it. It formed a seal and raised itself off the bottom of the jar. In the end I dried out most of the moisture and it is a rock hard nugget rattling in the jar. I bought a cheap loaf of malted brown bread which I am going to let go stale and hopefully PC it uncut in an AC bag

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Re: Growing on bread [Re: blackout]
    #4136268 -

Lol gross...

Diluded coffee right?

I thought I read in Adv cult. that regular brewed coffee might be too strong for the myc. (don't remeber what chemicals :frown:  )

I need some damn bread

-Gnostic

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Re: Growing on bread [Re: IGnosticAbhorI]
    #4136269 -

Bored but Thinking...

I've got some Stuffing siting next to me...It's white diced bread cubes..I was thinking about mixing it With some Cracked Powderized wheat I have in the fridge...Not sure to go about it...

I've got Plently of jars and LC's....so There's always room to experiment :grin:

-Gnostic

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Re: Growing on bread [Re: IGnosticAbhorI]
    #4136279 -

Very weak coffee, I read it should be good for invitro which is what I plan for these. The stuffing may end up a real mush when cooked wet. My bread shrunk a lot but it is still a bigger volume than 100g of wheat grains so I think it is still "airy". I think it would have been better if I did not wet the bread first. I think if I microwaved it dry it would not have shrunk as much and would have gone rock hard but would be more airy.

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Re: Growing on bread [Re: blackout]
    #4136289 -

"Very weak"...:thumbup:

Yea RR posted about it in Adv. Cult. back awhile ago with "explosive" invirto on quart jars...lol..I read allll about it...

Quote:
The stuffing may end up a real mush when cooked wet



Yea, it would...hmm.....What if I added just enought to wet it and then stuck it in the microwave to make them like rocks....then added a bit more till I got something airy and what I was looking for...

Damn, really going to have to play around with this idea to get it right :thumbup:

Gonna microwave and try what i said real quick and see what happens lol....

-Gnostic

1st attempt - Wow...That did mush up quite a bit....I think I'll add a bit of cracked wheat to help suck up a bit of the wetness...And maybe throw in some verm. as well....I think it'll end up being to putty like though...:shrug:

2nd - Added Less water...Not sure how much since i'm just Trying it out...The cubes stuck together a little bit but they're not mush...it looks like a bunch of bread cubes stuck together...but if u shake it hard enuf they break apart...i'ma add in some wheat and see what happens :thumbup:


Looks like it'll work, ima make a new thread and take pics sometime in the week if i'm able....see if it'll work :shrug:

Gl w/ the bread, hope to hear good news


Edited by IGnosticAbhorI (05/05/05 05:42 AM)

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Re: Growing on bread [Re: blackout]
    #4136308 -

Bread of any sort, once you try to hydrate it to 75% turns to a dough like consistancy, which is not a great thing for myc to colonize. I would wager that I could get a short stack of maybe 4 slices to fruit. BUT WHY... when there are a LOT BETTER substrate around.


--------------------

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Re: Growing on bread [Re: agar]
    #4136322 -

agar said:
BUT WHY... when there are a LOT BETTER substrate around.



To see if it is any good. Those other substrates were discovered by experimenting with growing on them, I have found very little info on growing on bread and just want to see the results, it is a probably the most readily available substrate around. If it is a disaster I will let people know.
I am also trying canned kidney beans, sweetcorn and peas at the moment. I have friends who would grow if it was easier and am looking for easy methods for me and them.

Edited by blackout (05/05/05 06:11 AM)

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Re: Growing on bread [Re: blackout]
    #4185284 -

The kidney beans are taking off really well, lots of growth in only 2 days from a LC. The peas are also looking good, again very dry I always tend to wet my grains too much so I don't want to add more liquid yet.
The sweetcorn has stalled and is definitely too dry, my lids are micron-magic filterdiscs which do let a lot of moisture escape. I have them in individual plastic bags and air them once a day now.

No growth on the bread at all, I think it is far too dry.

Edited by blackout (05/17/05 04:33 AM)

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Re: Growing on bread [Re: blackout]
    #4185291 -

it would ferment


--------------------

http://www.bruceeisner.com/ -Creating a Sensible Culture

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Re: Growing on bread [Re: liveby]
    #4185297 -

The yeast will be killed in sterilization, and end up as a usable nutrient.

Edited by blackout (05/17/05 04:39 AM)

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Re: Growing on bread [Re: blackout]
    #4185364 -

if you were to expose it to air as a casing layer it would ferment , because there are many millions of yeast spore flying around the room


--------------------

http://www.bruceeisner.com/ -Creating a Sensible Culture

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Re: Growing on bread [Re: liveby]
    #4194311 -

If thats the case then any substrate should "ferment" there is nothing magical about bread.

Here is a photo of the kidney beans and peas 4 days from innoc.

The picutres are not blurry it is the dried up residue from the beans and peas stuck to the sid eof the jars. They are more colonised that they appear since the residue has not colonised, the peas inside are all fully coated now.
There is a blob of silicon on the filters which is the self healing injection site

Edited by blackout (05/19/05 08:22 AM)

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