Home | Community | Message Board

Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Autoclave, Paul Stamets

Jump to first unread post. Pages: 1

Registered: 10/15/04
Posts: 210
Culture Transfer to Liquid
    #4034578 - 04/09/05 04:25 PM (11 years, 6 months ago)

Will this work?

I will plug a test tube half full of dextrose solution with an ear plug and sterilize it. I will take the largest gauge needle I can find and extract some mycelium from the center of a cake (so it is sterile). I will inject the mycelium into the liquid through the ear plug which will then heal itself. When the liquid culture is colonized I will suck it up with a sterile syringe and inoculate my jars.

Post Extras: Print Post  Remind Me! Notify Moderator
Feel Like aStranger...
 User Gallery

Registered: 11/07/04
Posts: 973
Last seen: 3 years, 9 months
Re: Culture Transfer to Liquid [Re: actionshroom]
    #4034626 - 04/09/05 04:39 PM (11 years, 6 months ago)

are you able to do agar transfers? i dont know much about the whole syringe thing but agar>liquid is tons easier then grain>liquid.. im not even sure you can do that, but i could be totally wrong... i cut my dishes and blend em up and inoculate.. each time.. no stir plate or anything, just blended mycelium into grain.. it adds a bit of moisture so i use as little water as possible.. i have a SS container for the blender i can autoclave which helps too.. don't know normal ratio's but with this method i use 3 dishes and inoculate 6 1/2gal jars and usually colonize within a week to ten days.. i know you can get way more mileage out of your mycelium with liquid fermentation techniques.. where you take the blended mycelium and instead of immedietly inoculating grain, adding to an enriched solution and stir it on a stir plate for 2-3days.. stir plates are lab equipment that usually cost hundreds of dollars but you can get one used for $20-30 on ebay.. this is a method paul stamets teaches and talks about in his book growing gourmet and medicinal mushrooms.. with this method he takes 5 dishes and in 3 days inoculates 100 1/2gal jars.. pretty amazing really... i know there are some simple teks or something here, im new here really.. check around, im sure people will post to.. this is one method that seems to work.. hope this info helps.. good luck!

"listening for the secret.. searching for the sound.."

Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Autoclave, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Liquid culture from Liquid culture. matits 5,246 19 07/13/13 01:34 AM
by MattyBear
* making liquid cultures from liquid cultures and so on... Drcircuits 603 4 11/01/06 09:34 AM
by Blutjager
* what is a "tissue-culture treated" petri dish? Question on buying dishes imnotthere 562 1 10/12/08 08:44 PM
by veda_sticks
* PF Cake to Liquid culture transfer? Rohypnol 1,139 5 10/25/03 07:05 PM
by Hanky
* Transferring liquid like a pro? pontus 391 4 08/16/08 06:25 PM
by Juke Adro
* Colonizing petri dishes, what can i do with them now ?
( 1 2 all )
WakingUp 2,514 21 06/25/08 07:26 AM
by ScavengerType
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 all )
agar 24,832 38 12/05/11 11:40 PM
by soicyboy121
* Old Timer's method Dvoid 2,056 12 05/09/01 11:33 PM
by Hippie3

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
503 topic views. 23 members, 93 guests and 17 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.001 seconds on 14 queries.