Culture Transfer to Liquid

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actionshroom
RookieMycologist

Registered: 10/15/04
Posts: 210

Culture Transfer to Liquid

#4034578 - 04/09/05 04:25 PM (21 years, 6 months ago)

Will this work?

I will plug a test tube half full of dextrose solution with an ear plug and sterilize it. I will take the largest gauge needle I can find and extract some mycelium from the center of a cake (so it is sterile). I will inject the mycelium into the liquid through the ear plug which will then heal itself. When the liquid culture is colonized I will suck it up with a sterile syringe and inoculate my jars.

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mattymonkey
Feel Like aStranger...

Registered: 11/07/04
Posts: 973
Last seen: 13 years, 9 months

Re: Culture Transfer to Liquid

[Re: actionshroom]
#4034626 - 04/09/05 04:39 PM (21 years, 6 months ago)

are you able to do agar transfers? i dont know much about the whole syringe thing but agar>liquid is tons easier then grain>liquid.. im not even sure you can do that, but i could be totally wrong... i cut my dishes and blend em up and inoculate.. each time.. no stir plate or anything, just blended mycelium into grain.. it adds a bit of moisture so i use as little water as possible.. i have a SS container for the blender i can autoclave which helps too.. don't know normal ratio's but with this method i use 3 dishes and inoculate 6 1/2gal jars and usually colonize within a week to ten days.. i know you can get way more mileage out of your mycelium with liquid fermentation techniques.. where you take the blended mycelium and instead of immedietly inoculating grain, adding to an enriched solution and stir it on a stir plate for 2-3days.. stir plates are lab equipment that usually cost hundreds of dollars but you can get one used for $20-30 on ebay.. this is a method paul stamets teaches and talks about in his book growing gourmet and medicinal mushrooms.. with this method he takes 5 dishes and in 3 days inoculates 100 1/2gal jars.. pretty amazing really... i know there are some simple teks or something here, im new here really.. check around, im sure people will post to.. this is one method that seems to work.. hope this info helps.. good luck!

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