Home | Community | Message Board


Kratom Eye
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Potato Dextrose

Jump to first unread post. Pages: 1
OfflineDrWild
mysterioustraveler
Registered: 04/17/01
Posts: 52
Last seen: 11 years, 10 months
Potabello Mycillium on Agar
    #391857 - 09/12/01 01:02 AM (15 years, 2 months ago)

I took a print from a port a got at the store. Im trying to get the spores going on Sabaraunds (sp) Agar. On the few attempts that I try, I have gotten a blue-green mold colony overgrowth. I cant even seem to get it seperated out by dilution/streaking.

Any ideas/suggestions?

Happy Trails,
DrWild


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 12 days
Re: Potabello Mycillium on Agar [Re: DrWild]
    #393060 - 09/13/01 05:26 AM (15 years, 2 months ago)

Clone the mushroom, it?s far easier.
Use peroxide agar, if you don?t have a flowhood.

Read

http://www.mykoweb.com/articles/cultivation.html



-------------------
Visit www.fungifun.org formerly kickme.to/mushrooms

Be very, very careful what you put into that head,
because you will never, ever get it out.

Thomas Cardinal Wolsey (1471-1530)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDrWild
mysterioustraveler
Registered: 04/17/01
Posts: 52
Last seen: 11 years, 10 months
Re: Potabello Mycillium on Agar [Re: Anno]
    #394750 - 09/15/01 12:19 AM (15 years, 2 months ago)

Great idea! Great link too!

I never tried cloning before... but it I should be able to pull it off.

When you say peroxide agar, do you mean that I should add peroxide to the PDA or MDA agar? Im not really sure what you mean here, as I just scanned the site that you refered me too. I'll give it a thorough read tomorrow.

Thanks for your help. :-)





Happy Trails,
DrWild


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 12 days
Re: Potabello Mycillium on Agar [Re: DrWild]
    #394805 - 09/15/01 02:13 AM (15 years, 2 months ago)

Read this http://www.theforestfloor.org/archives/old_archive/peroxide_agar_tek.html.

-------------------
Visit www.fungifun.org formerly kickme.to/mushrooms

Be very, very careful what you put into that head,
because you will never, ever get it out.

Thomas Cardinal Wolsey (1471-1530)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDrWild
mysterioustraveler
Registered: 04/17/01
Posts: 52
Last seen: 11 years, 10 months
Re: Potabello Mycillium on Agar [Re: Anno]
    #396129 - 09/16/01 09:09 PM (15 years, 2 months ago)

Thanks Anno!

I really appreciate your help. I'll post and let ya know the results of the experiment.



Happy Trails,
DrWild


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDrWild
mysterioustraveler
Registered: 04/17/01
Posts: 52
Last seen: 11 years, 10 months
Re: Potabello Mycillium on Agar [Re: DrWild]
    #447196 - 11/04/01 11:56 PM (15 years, 1 month ago)

An update on the project.

I bought a port at the grocery store. Took some tissue samples. They cultured nicely to agar! The tissue samples from the region of the cap and the gills grew the best. I generally got poor results from tissue samples removed from the stem.

Anyhow, I innoculated 3 quart jars of malted barley (a friend had it left over from a beer-making project). The culture took in only one of the jars. Never could get the other 2 to grow... even after re-innoculating.

Anyhow... Im about to case in the next day or so... any suggestions would be helpful. Im planning on straw and composted manure.

Thanx much.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Similar ThreadsPosterViewsRepliesLast post
* Reishi spores ---> BRF agar substitute zeegos 2,140 18 10/30/06 11:25 PM
by CptnGarden
* Agar->LC trich_cultivator 1,079 13 12/19/08 09:02 PM
by PitcherCrab
* King Oyster - Wedge to Agar joehinkle 1,196 15 05/28/09 12:50 AM
by Jef
* Do you reuse the glass containers that you pour agar out of?
( 1 2 all )
jjb007 1,952 23 01/21/09 01:53 PM
by denger
* cloning a storebought oyster w/o agar? kingjames488 1,615 15 04/29/09 03:11 PM
by jjb007
* Why do not make a cumulative order for agar powder and light malt extract in powder?
( 1 2 all )
FreeSporePrints 4,774 34 11/25/12 07:38 AM
by sparkle
* potato agar....
( 1 2 all )
trich_cultivator 2,568 27 12/11/08 05:40 PM
by trich_cultivator
* Liquid and agar culture of H. ulmatius? Baby_Hitler 1,064 4 05/19/04 10:41 AM
by charlieXxXxX

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, EvilMushroom666, cronicr, Pastywhyte
1,479 topic views. 5 members, 12 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Sporeworks
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.002 seconds on 14 queries.