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OfflineDrWild
mysterioustraveler
Registered: 04/17/01
Posts: 52
Last seen: 19 years, 2 months
Potabello Mycillium on Agar
    #391857 - 09/11/01 11:02 PM (22 years, 7 months ago)

I took a print from a port a got at the store. Im trying to get the spores going on Sabaraunds (sp) Agar. On the few attempts that I try, I have gotten a blue-green mold colony overgrowth. I cant even seem to get it seperated out by dilution/streaking.

Any ideas/suggestions?

Happy Trails,
DrWild

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OfflineAnnoA
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Re: Potabello Mycillium on Agar [Re: DrWild]
    #393060 - 09/13/01 03:26 AM (22 years, 7 months ago)

Clone the mushroom, it?s far easier.
Use peroxide agar, if you don?t have a flowhood.

Read

http://www.mykoweb.com/articles/cultivation.html



-------------------
Visit www.fungifun.org formerly kickme.to/mushrooms

Be very, very careful what you put into that head,
because you will never, ever get it out.

Thomas Cardinal Wolsey (1471-1530)

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OfflineDrWild
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Registered: 04/17/01
Posts: 52
Last seen: 19 years, 2 months
Re: Potabello Mycillium on Agar [Re: Anno]
    #394750 - 09/14/01 10:19 PM (22 years, 7 months ago)

Great idea! Great link too!

I never tried cloning before... but it I should be able to pull it off.

When you say peroxide agar, do you mean that I should add peroxide to the PDA or MDA agar? Im not really sure what you mean here, as I just scanned the site that you refered me too. I'll give it a thorough read tomorrow.

Thanks for your help. :-)





Happy Trails,
DrWild

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OfflineAnnoA
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Re: Potabello Mycillium on Agar [Re: DrWild]
    #394805 - 09/15/01 12:13 AM (22 years, 7 months ago)

Read this http://www.theforestfloor.org/archives/old_archive/peroxide_agar_tek.html.

-------------------
Visit www.fungifun.org formerly kickme.to/mushrooms

Be very, very careful what you put into that head,
because you will never, ever get it out.

Thomas Cardinal Wolsey (1471-1530)

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OfflineDrWild
mysterioustraveler
Registered: 04/17/01
Posts: 52
Last seen: 19 years, 2 months
Re: Potabello Mycillium on Agar [Re: Anno]
    #396129 - 09/16/01 07:09 PM (22 years, 6 months ago)

Thanks Anno!

I really appreciate your help. I'll post and let ya know the results of the experiment.



Happy Trails,
DrWild

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OfflineDrWild
mysterioustraveler
Registered: 04/17/01
Posts: 52
Last seen: 19 years, 2 months
Re: Potabello Mycillium on Agar [Re: DrWild]
    #447196 - 11/04/01 09:56 PM (22 years, 5 months ago)

An update on the project.

I bought a port at the grocery store. Took some tissue samples. They cultured nicely to agar! The tissue samples from the region of the cap and the gills grew the best. I generally got poor results from tissue samples removed from the stem.

Anyhow, I innoculated 3 quart jars of malted barley (a friend had it left over from a beer-making project). The culture took in only one of the jars. Never could get the other 2 to grow... even after re-innoculating.

Anyhow... Im about to case in the next day or so... any suggestions would be helpful. Im planning on straw and composted manure.

Thanx much.

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