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OfflineBaby_Hitler
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Should A. bisporus be any color than white on peroxide agar?
    #1811670 - 08/14/03 12:40 AM (14 years, 6 months ago)

About 3-4 days ago I droped some of those "mushroom seeds" I got a few months ago on a dish of peroxidated agar. Some white fuzz is coming out of one of the seeds and is colonizing the agar. It looks grey where it comes in contact with the agar. Some of the other seeds are developing bluish/grayish/greenish spots.

Is any of this A. bisporus?


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[quote]RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.

Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color.  By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish.  Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate.  Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]


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Re: Should A. bisporus be any color than white on peroxide agar? [Re: Baby_Hitler]
    #1812183 - 08/14/03 04:08 AM (14 years, 6 months ago)

Could be, its quite wispy to start, becoming more cottony
when it thickens, green patches dont sound to good though.
But a grey tint is normall on mine. Also a purple tint to
the chestnut buttons.


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OfflineBaby_Hitler
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Re: Should A. bisporus be any color than white on peroxide agar? [Re: Paid]
    #1812247 - 08/14/03 04:31 AM (14 years, 6 months ago)

It's like a thin layer of black right at the surface if the agar where it meets the mycelium.


--------------------
[quote]RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.

Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color.  By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish.  Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate.  Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]


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Re: Should A. bisporus be any color than white on peroxide agar? [Re: Baby_Hitler]
    #1812590 - 08/14/03 08:01 AM (14 years, 6 months ago)

Can you section of a clean part?


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OfflineBaby_Hitler
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Re: Should A. bisporus be any color than white on peroxide agar? [Re: Paid]
    #1814148 - 08/14/03 05:35 PM (14 years, 6 months ago)

Dunno if there is a clean part, or a dirty part. I picked out all the colored bits. They do not appear to have infected the agar. I need to know if this grey/black could be caused by A. bisporus.





--------------------
[quote]RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.

Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color.  By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish.  Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate.  Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]


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Re: Should A. bisporus be any color than white on peroxide agar? [Re: Baby_Hitler]
    #1814312 - 08/14/03 06:19 PM (14 years, 6 months ago)


Is The White Button.


Is the chestnut button.

So i would say its not unfeazerbal. But it don't look nice :tongue: 


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OfflineBaby_Hitler
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Re: Should A. bisporus be any color than white on peroxide agar? [Re: Paid]
    #1814722 - 08/14/03 08:20 PM (14 years, 6 months ago)

http://www.shroomery.org/forums/showflat...rue#Post1600479

Quote:

Anno said:
Cloned from supermarket 10 days ago on potato water-oatmeal-sucrose agar. Notice how the A. bisporus is altering the color of agar. This is NOT bacterial contamination.






That looks just about like what I've got, except his are more brownish, and mine are more greyish, and his mycelium looks more ropey and mine looks more poofy and fuzzy.


--------------------
[quote]RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.

Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color.  By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish.  Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate.  Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]


Edited by Baby_Hitler (08/14/03 08:25 PM)


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Re: Should A. bisporus be any color than white on peroxide agar? [Re: Baby_Hitler]
    #1815815 - 08/15/03 03:11 AM (14 years, 6 months ago)

Yeah, they do seem pretty varierbal, the chestnut button makes
the agar purple. It does look like mycelium coming directly from the
seeed. So i would go with it for now.
Good luck with em.


--------------------



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OfflineMycena
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Re: Should A. bisporus be any color than white on peroxide agar? [Re: Paid]
    #1819627 - 08/16/03 01:35 PM (14 years, 6 months ago)

My experience

When you clone Buttons they tend to bruise badly. This is even more pronounced on peroxide . Eventually some will start to show regrowth, some will contam. Like anno said a few if not all will bleed a pigment into the agar. generall this is not good but bleeders canstill recover albeit slower.
sub the ones that are wispy at the edges.
On fresh agar growth should speed up and be more rhizo, and by the third sub the culture will be white and a normal agaricus culture

Interestingly i threw out som contam subs only to find a month later they hadnt quite made it to the bin. The agricus had crawled over and eaten the contaminanats - i guess thats its nature as a secondary decomposer


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