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Offlinetaters
french fried

Registered: 11/03/02
Posts: 82
Loc: the garden
Last seen: 19 years, 1 month
agar problems
    #1500486 - 04/28/03 11:12 AM (21 years, 1 month ago)

I am STILL trying to get the hang of agar.

5 days ago I inoculated 4 birdseed jars with Pink Oyster wedges. Since I suspected that the oyster plate used was contaminated, I put wedges onto 6 new peroxidated PDA plates as well.

Today I found that all the birdseed jars had nice growth, although two of them had the green. Of the 6 PDA plates, NONE are showing growth.

Would the proper amount of peroxide slow growth that much? Was PDA a poor choice for oysters? Or is it more likely that I just fucked up the recipe?  :confused:

I'm almost out of wedges... if I mess up again I'll lose my oysters.  :frown:   

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OfflinePaid
Pict
 User Gallery

Registered: 03/12/03
Posts: 5,376
Loc: Zone ate
Last seen: 20 years, 4 months
Re: agar problems [Re: taters]
    #1500558 - 04/28/03 11:54 AM (21 years, 1 month ago)

If youve moved to pda from another agar recipe, then it may take a bit to adjust to the new agar, as well as the peroxide.What temp do you have them at?
And do you think you could have over boiled the potatoes?As i did this once and it slowed everything down.


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Offlinetaters
french fried

Registered: 11/03/02
Posts: 82
Loc: the garden
Last seen: 19 years, 1 month
Re: agar problems [Re: Paid]
    #1500909 - 04/28/03 02:10 PM (21 years, 1 month ago)

I really do not know what the previous aqgar recipe was, the plate was given to me by a friend. Incubaton fluctuates between 79F and 84F. If it's possible to over boil potatoes then I probably did it.  :crazy:

I'll give these plates another 5 days or so... and for my final attempt I'll us MEA. Thanks bro. 

edit: that ever changing chick cracks me up.

Edited by taters (04/28/03 02:12 PM)

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Invisibleshaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
Re: agar problems [Re: taters]
    #1501237 - 04/28/03 03:58 PM (21 years, 1 month ago)

try dog food agar DFA 10grams dog food - 10 grams agar - 500 ml water. and poof!

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Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 years, 9 months
Re: agar problems [Re: taters]
    #1506921 - 04/30/03 06:22 AM (21 years, 1 month ago)

You should wait more than 5 days, it takes some time to recover.
Pink oysters sometimes switch to "fruit mode" and start slowly to develop primordia on agar, even before the plate if fully colonized.

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Offlinetaters
french fried

Registered: 11/03/02
Posts: 82
Loc: the garden
Last seen: 19 years, 1 month
Re: agar problems [Re: taters]
    #1519122 - 05/04/03 09:17 AM (21 years, 1 month ago)

My Pink Oysters are safe.

After 5 more days of no growth, I mixed up a batch of MEA and tried again with the last of the wedges. Several plates are now growing nicely.  :laugh:

I must have REALLY messed up that PDA; not even the green will grow on it.  :grin:

 

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