Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Mushroom-Hut Substrate Mix   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1 | 2 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Agar recipe for cloning oysters "UPDATE PICS 16/12"
    #2142356 -

allright, so i prepared some PDA h202 agar, went to the store and bought some fresh oyster shrooms. Cut a piece off the stem (inside) with a clean scalpel, placed the piece on the agar. Its been 6 days and there is no growth on the agar.............. but the transferred piece is fluffy.

Is there a problem with oysters and h2o2? I gave some plates to a friend, he cloned some cubes (at the same time as i did the oysters) and there is plenty of growth on the cube plates...but the oysters doesnt like the pda agar or what?
Is there a special recipe for oysters??

Need some advice here...

Edited by Wheedhead! (12/16/03 03:44 PM)

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: Wheedhead!]
    #2142368 -

well,
1) it takes a few days for mycelium to get used to h202, that might be the problem
2) could just be a poor strain to clone, I've heard that alot of store-bought species specially made to be especially hard to clone ( cubies arent like that imo)

personally i haven't used pda so I can't help you in that respect, but i find oysters like DFA.. heh.



Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: ]
    #2148610 -

Oysters don't have problems with H2O2 and the standard recipies work fine.
It's possible that you got contamns around the transfered piece, that's why it's getting fluffy but it's not spreading on agar. Try to transfer the "fluffy" part to new dishes.

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: zeronio]
    #2149018 -

The piece will fluff up before it starts to grow on the agar. Sometimes h2o2 will cause a bit of lagtime before the mycelium starts to colonize the plate.

patience...........

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: Millet]
    #2149151 -


Its been 10 days now with zero growth on the Agar, the cubes are doing just fine though.


--------------------
I just  :heart: The Shroomery

Monkeys might fly out of my butt

Dope will get you through times of no money better than money will get you through times of no dope. "
Fabulous Furry Freak Brothers



Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: Wheedhead!]
    #2149235 -

Well, maybe the oysters were beyond cloning, due to age.
I have cloned many shop bought oysters on

Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar

with .84 ml of 17% h2o2 per 500ml

and oysters seem to love this, with growth
in days, is there not even any mycelium fluff
on the flesh pieces them selve? As if not, i would say start
again.

Better luck next time mate.


--------------------
er

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: G a n j a]
    #2151091 -

I've had better luck with MEA. Was the tissue from the base of the stem? Try to use tissue from the cap instead.

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: G a n j a]
    #2151233 -


The pieces are all nice fluffy, but the myc is not spreading. Guess ill have to wait, meantime i think ill prepare some new plates and buy the oysters from a diferent shop (maybe i should ask the manager if he would call me when they get a fresh delivery of shrooms?) and make some new transfers.

some of the pieces transferred were cut from the stem and others from the cap.

The only mistake i know i made was to use too big chunks of shroom. But that should not prevent growth??? or??



--------------------
I just  :heart: The Shroomery

Monkeys might fly out of my butt

Dope will get you through times of no money better than money will get you through times of no dope. "
Fabulous Furry Freak Brothers



Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: Wheedhead!]
    #2151342 -

IME, even if there was some bacteria hitching a ride, the culture would still grow across the agar. When you look at the plate from underneath, does there appear to be bacterial growth? [odd-looking patches]. You may not see them if the plate was poured thick.
Because it is 'fluffing up', it sounds like it's trying to go back into vegetative growth. It could be poor/old genetics or possibly the agar formulation. . .vigorous oyster cultures will colonize just about any standard media. I'd recommend hanging on to the plates [for observation] and trying again with a new clone and new plates.

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: Wheedhead!]
    #2157292 -

Quote:
The pieces are all nice fluffy



sounds like you just need to wait a bit longer, once
theyve fluffed up, its just a matter of time before
it moves onto the agar.


Good luck.


--------------------
er

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: G a n j a]
    #2166716 -

Just wait a while longer. It took me 3 weeks using store bought oysters (I also made the mistake of using big chunks, there was 50% bacteria contamination) to colonize about half of a dish (PDYA, with peroxide) but after that it seemed to speed up a bit. For about 2 weeks it stayed on the flesh but soon it spread out.

On the other hand, maybe my strain is also not so vigorous - its been 10 days on white wheat in quart jars (having been shaken twice) and the growth looks nothing like what it looks like in pictures.

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: keela]
    #2169176 -

Ladies and gentlemen.............we have growth.

Yesterday i checked the plates, and they were growing  :laugh: Nice pure white mycelium. Today the growth has doubled and i think the plates will be fully colonised within a wekk or two. I will post some pics later (just have to move my lazy ass and get my camera at a friends house)

Thank you people for telling me to keep the plates, I was loosing hope :wink:

Now I have to decide what substrate to use for fruiting, im thinking agar to popcorn (spawn) and then plain straw(substrate)? Any ideas?
Also I ould like to know the exact incubation temp and the fruiting conditions for oysters (I know I need the bible,,TMC,, its on my christmas wish list )

I have been searching the shroomery for pictures of oyster myc on agar to compare my experiment, but i didnt find any. So if anybody has an url or remembers a post on this subject, please share.

I just  :heart: The Shroomery

Edited by Wheedhead! (12/08/03 03:15 PM)

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: Wheedhead!]
    #2169464 -

TMC gives Pleurotus ostreatus as
Spawn run :78-84?f

Pinning : 55-60?f

High humiderty through out.

Good luck, I would suggest plan straw as well, make sure
its well chopped up, as it makes a better block, the smaller
the straw is shredded IME.

:thumbup:


--------------------
er

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: G a n j a]
    #2169483 -



Is a P. pulmonarius



Is a clone from Anno which is a P. ostreatus I think


(I could be wrong, I can't find the paper work lol I'll
double check tomorrow, but there def oysters)


--------------------
er

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: G a n j a]
    #2171348 -

I used potato dextrose agar i made in an oven to clone oysters recently as well, i only inoculated them four days ago and i've already got growth on the agar, although no fluffing.


Okay guys, this hasn't much to do with it but i need to know something. What is the optimum temperature to add h2o2 to the agar? i'm assuming if you add it while it's too hot it's going to deteriorate rapidly and basically become hydrogen and oxygen with no anti-contam effects. If anyone knows the best temp or at least a proven temp let me know. (i have had success by just guessing based on how hot it feels, but i'd rather be precise)


--------------------
"Preacher man don't tell me, heaven is under the earth, 'cause i know you don't know, what life is really worth..." ~ The Wailin' Wailers

I grow legitimate mushrooms only.

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: Indubitableness]
    #2171369 -

130 F will be cool, at 140 F the H2O2 will break down and you will loose some of the benifits (if not all) of the addition. Go for 6cc per litre of agar.
hope I was helpful
puggy :rasta:

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: Indubitableness]
    #2171371 -

130?f or 55?c is when i add it, it got that from here
http://www.shroomery.org/index/par/23462
and it works well for me.


--------------------
er

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: G a n j a]
    #2171378 -

be carefull of your h2o2 % before doing the math. mines 17% but then 9% and 3%
are also common.


--------------------
er

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: G a n j a]
    #2171962 -

ganja,
shit i totally forgot about that, i was just assuming everyone was buying the same stuff i was. (oops:) what I was using is 3%
puggy

Extras: Filter Print Post Top
Re: Agar recipe for cloning oysters [Re: puggymalone]
    #2172016 -

You should be fine then, as I think most of the
formulars are set for the 3% :thumbup: but its
worth being aware of :grin:


--------------------
er

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 |  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Mushroom-Hut Substrate Mix   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Do shiitakes prefer any particular agar recipe? Lothar 548 3 12/12/21 02:50 PM
by liamtheloser
* Cloning oysters Mushroomklown 1,196 1 09/15/05 08:01 AM
by eris
* cloning store bought mushrooms
( 1 2 all )		 thisone 9,543 25 12/01/03 11:29 AM
by Bi0TeK
* oysters and pink oyster? staindblue 4,772 9 05/31/02 07:49 PM
by staindblue
* Oyster Straw Log
( 1 2 3 all )		 RogerRabbitM 20,675 56 10/14/10 10:55 AM
by BlackPeace
* strange behaviour of my oyster Quincunx 2,740 13 02/23/04 11:41 AM
by Quincunx
* Need Help for Cloning and More...
( 1 2 all )		 micololo2 4,369 28 09/02/06 07:27 AM
by micololo2
* Oyster Clone IN PEt Bedding Q xburn 1,240 3 04/27/05 04:51 PM
by ToolTroll

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
9,979 topic views. 0 members, 51 guests and 18 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.008 seconds on 16 queries.