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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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substrate too hard
    #351876 -

After pressure cooking a recent batch of jars filled with vermiculate based substrate for an hour , i noticed that the substrate seemed far too hard when i went to inject?? What happened??



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Re: substrate too hard [Re: Zen Peddler]
    #352075 -

You may have baked them dry.


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Re: substrate too hard [Re: Zen Peddler]
    #352085 -

make sure you somehow stop water from evaporating out of your jars. i like to use foil tape. just cut into squares and put over the holes. i've had great luck with that.

wiping the webs and the dew from my withered eye


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Re: substrate too hard [Re: Zen Peddler]
    #352187 -

Put a washcloth or rag under the jars next time...the water had probably evaporated from them comming in contact to the very bottom of the cooker.

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively..."


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Re: substrate too hard [Re: Shroominator]
    #352208 -

To cut down on evaporation during sterilization, just TAPE UP YOUR HOLES. I use waterproof plastic packaging tape, which doesn't come off or buckle during the PC-ing / boiling process. Then I poke the needle through it when innoculating and tape over the puncture holes to seal the jars again.

I am a figment of my own imagination
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I am a figment of my own imagination
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Re: substrate too hard [Re: wind_wraith]
    #352242 -

actually i always put a cloth on the bottom of the pressure cooker. Yeah, im going to try and tape up the holes while i sterilise. Im not sure how your mycelium can breath if you retape the holes...never worked for me...



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Deleted [Re: Zen Peddler]
    #352257 -

Content Removed.

Edited by Jared (04/01/04 01:13 AM)

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Re: substrate too hard [Re: Jared]
    #352380 -

you have to take the scotch tape off after pcing and before innoculating so it can have air exchange to colonize correct? Or am I confused?



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Re: substrate too hard [Re: thegoldenteacher]
    #352394 -

if one were to use scotch tape would he also have to use tin foil when p.cing?
thanx
ted

http://ted_bundy_jr.tripod.com you have been warned


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http://ted_bundy_jr.tripod.com you have been warned

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Re: substrate too hard [Re: thegoldenteacher]
    #352401 -

Wouldnt the tape create a dangerous pressure build up in the jars
while theyre cooking? It seems like just putting some foil over the jar
would prevent moisture from entering and escaping through the holes.
In reply to:

you have to take the scotch tape off after pcing and before innoculating so it can
  have air exchange to colonize correct? Or am I confused?


I think the tape should come off after you have some colonization.:)

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........|...|Free
...__ /.../..Spore
...|___ / .....Ring

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Re: substrate too hard [Re: Zen Peddler]
    #352445 -

Probably packed them too tight #1

if you're pressure cooking BRF/verm half pints, only 20 minutes is needed. one hour for steaming

have to cover them with foil while cooking to keep water from leaking in

if you opened the pc before it was down to 0 pressure or "quick cooled" it under water the moisture would have boiled out

some possibilities


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Re: substrate too hard [Re: Zen Peddler]
    #352458 -

I used waterproof first aid tape with breeding microholes (the same you use when you get injured) to prevent possible high pressure inside the jars, i tested the substrate water content after PC'ing, it might need i bit more water (+10ml per quart jar) because of a little water loss to the outside but it's still OK to innoculate. I put aluminum foil then secured the foil with rope. After PC'ing, the foil had formed a dome indicating presure leak from the inside of jar. I have one jar made this way with 40% mycellium growth after 14 days, no contams and with consistent substrate.
The microholes also let the mycellium breed during growth time. I'll be using this from now on.

Cheers,
MAIA

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"Pictures in a box at home
Yellowing and green with mold
So i can barely see your face
Wonder how that color taste"
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Edited by MAIA on 07/01/01 02:12 PM.



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Re: substrate too hard [Re: MAIA]
    #352462 -

Band-aids.. got to get me some.:)

....___^___
...(_______)
........|.|Free
...__ /../..Spore
...|__ / ......Ring


--------------------
.....___^___
....(_______)
........|...|Free
...__ /.../..Spore
...|___ / .....Ring

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Re: substrate too hard [Re: Nagual]
    #352807 -

About pressure buildup in taped-shut jars: no, the jars won't explode; the plastic (or what have you) is weaker than the glass and would blow out long before the glass breaks.  But hey, wear safety goggles if you're worried :wink:.
I always re-tape my jars and they colonize just fine; air exchange may speed up colonization, I don't know, but it doesn't seem to be necessary.  And taping your holes ensures that no nasty contams are going to waft in and ruin your cultures before they're done colonizing.

I am a figment of my own imagination
http://go.to/FreeSporeRing


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I am a figment of my own imagination
http://go.to/FreeSporeRing

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Re: substrate too hard [Re: Nagual]
    #352832 -

Just make sure they're waterproof.

__
"Pictures in a box at home
Yellowing and green with mold
So i can barely see your face
Wonder how that color taste"
=)Free&Spread the Spores(=
Support the FSR


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

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Re: substrate too hard [Re: MAIA]
    #352887 -

BTW, the microholes only let the air out, so no need to worry about contams getin inside.

__
"Pictures in a box at home
Yellowing and green with mold
So i can barely see your face
Wonder how that color taste"
=)Free&Spread the Spores(=
Support the FSR


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

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Re: substrate too hard [Re: MAIA]
    #352891 -

Duct tape, cover all four holes at once. Fold the ends in to make tabs.


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Re: substrate too hard [Re: puscle]
    #353153 -

No, duct tape's no good, if you're trying to prevent your water from boiling off, anyway. It buckles up along the grid lines and breaks the seal in a dozen places. I haven't tried ordinary masking tape, though... I assumed it would come right off in boiling water, sticky paper that it is.

I am a figment of my own imagination
http://go.to/FreeSporeRing


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I am a figment of my own imagination
http://go.to/FreeSporeRing

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Shop: Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   MagicBag.co Certified Organic All-In-One Grow Bags   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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