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Stabilizing a Mutant Strain?
    #3487409 -

I think mutants look cool, so i would like a strain that always gave mutants. Is this even possible? If so would you 1st make a clone of a mutant and see if that makes more mutants and then take a spore print to see if the spore will grow more mutants, or just skip the cloning part? Any suggestions or input would be appreciated.


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life is one big question when your starin at the clock
And the answers always waiting at the liqour store, 40 oz to
Freedom,
so I'll take that walk.
And I know that ohhhh...I'm not comin back

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Re: Stabilizing a Mutant Strain? [Re: NoodleSalad]
    #3487504 -

Im by no means even somewhat experianced in a subject like this, but if you do too many g2g transfers or kero to kero you start to get mutants after so many generations.

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Re: Stabilizing a Mutant Strain? [Re: NoodleSalad]
    #3487704 -

The clone would be the likely candidate for producing more mutants. If you go back to spores, the mushrooms will most likely have regular charateristics. But, you want to make sure this would be a genetic mutation instead of one caused by chemicals. The latter would probably revert to regular mushrooms in the absence of said chemicals...


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To give is to live...


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Re: Stabilizing a Mutant Strain? [Re: ATWAR]
    #3487820 -

So if i did enough transfers that i was consistently getting mutants, and i took a spore print do you think that spore print would produce mutants? If so, would those mutants spore prints produce mutants?


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life is one big question when your starin at the clock
And the answers always waiting at the liqour store, 40 oz to
Freedom,
so I'll take that walk.
And I know that ohhhh...I'm not comin back

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Re: Stabilizing a Mutant Strain? [Re: NoodleSalad]
    #3487919 -

Mutants are usually difficult to get prints from.

eg. Malaysians... I've done, have always thrown mutants.
Out of 4 casings, I only got about 8 prints.
The fruit from those prints were also mutants.
I don't know if this is true of other strains mutants, but it seems so for the Malaysians.

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Re: Stabilizing a Mutant Strain? [Re: NoodleSalad]
    #3488637 -

NoodleSalad said:
So if i did enough transfers that i was consistently getting mutants, and i took a spore print do you think that spore print would produce mutants?



If you were to intentionally over-transfer mycelium to obtain mutants, I believe the first thing that is lost is the ability to produce spores (reproduce). So in effect, you would be creating sterile mutants...


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To give is to live...


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Re: Stabilizing a Mutant Strain? [Re: NoodleSalad]
    #3490152 -

I've heard that a very light swipe of vaseline on the developing caps will induce mutants. Dunno if it's really true. If you're only interested in making it happen, without waiting generations, it might be worth a try.

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Re: Stabilizing a Mutant Strain? [Re: Suntzu]
    #3596103 -

Suntzu, is this true? I have some interest in mutants also and I will surely try it out. (Are you jerkin' my donger?)

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Re: Stabilizing a Mutant Strain? [Re: largosnook]
    #3598367 -

I'd never jerk your donger.  I'm telling you the truth when I say I don't know if it works :wink:
If it does work, it wouldn't be making true 'mutants', just deformed fruits.

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Re: Stabilizing a Mutant Strain? [Re: Suntzu]
    #3605363 -

maybe if u cloned a mutant and just kept growing from that clone. u always could just use UVB for ur lighting... thatd make a normal strain put up basically nothing but mutants.


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Re: Stabilizing a Mutant Strain? [Re: NoodleSalad]
    #3616812 -

Pretty much impossible actually.
The problem is that every mushroom is a representation of one of the possible phenotype variants that its particular spore match can exhibit as a response to varied environmental parameters. In a nutshell, when a mushroom begins to form, it has to have a few different variations in its characteristics to be able to respond to differing environmental parameters.
Mutants are usually just an expression of an inappropriate phenotype expression showing up from myriad of possible variants possible from its genetics.
Even if you clone it, you maye get one or two fruits out of a flush that are similar, but never a whole flush that are exactly the same. Genetics are never stable, even when you are cloning.
Its sort of like cloning a human who has lived in the arctic his whole life and eaten nothing but fatty foods and comparing to his clone 40 years down the track, who is an athelete who lives in california.


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Re: Stabilizing a Mutant Strain? [Re: Zen Peddler]
    #3763626 -

why don't you just keep them under a blacklight?....my understanding from the spores is that if you are able to get spores those should have genes that are pure, and stable....so I doubt they would have any mutant charcteristics


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the jent

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Re: Stabilizing a Mutant Strain? [Re: jent]
    #5288319 -

1.Take wanted clonee
2.Make three clones
Make mycelium syringe of each clone
3.Inoculate three separate cakes and grow(if you want you can put colonized cake into a casing).
4.Clone best from each cake/casing
5.Inoculate three separate cakes and if you want turn into casings
6.Take spores from most desirable mushroom of each cake/casing
7.Combine and there is now an isolation of whatever you want?.

or something like that

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Re: Stabilizing a Mutant Strain? [Re: cultzombieflik]
    #5290624 -

I would think about it this - a mutation is a direct corruption of the Dna/Rna of a species usually caused by some external variable that the species has been in contact with during its development.
To expect that spores or a clone of such a mutation would hold the same genetic corruption - and that corruption to be stable enough for this to occur is unlikely.
It is posible however - some cancers in humans are a direct result of hypermethylation of certain genes - and this tendency can run in families.
Its important to remember that a clone of a mushroom is not the same as a clone of a human being - when you clone a mushrooms its like taking a cutting from a plant - it increases the likelyhood that any growth from that time will exhibit a similar phenotype expression to its cloned source but its by no means a guarantee.
If you go back to spore then its far less likely - your looking at hoping that a mutation will occur and that one of say 2 million possible phenotype characteristics will randomly re-appear in exactly the same way.
Your far better off trying to work out what caused the mutation and repeating this on newer batches or just cloning live mushroom tissue


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Re: Stabilizing a Mutant Strain? [Re: cultzombieflik]
    #5290631 -

you wish it was that easy :wink:


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Re: Stabilizing a Mutant Strain? [Re: cultzombieflik]
    #5293402 -

NO!

Just no. Read up on genetics if you actually believe this.


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You can't hold a man down without staying down with him.
-- Booker T. Washington

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Re: Stabilizing a Mutant Strain? [Re: DocPsilocybin]
    #5305427 -

Just spray lysol over it every few days,lol. JK

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Re: Stabilizing a Mutant Strain? [Re: FungusMan]
    #5336426 -

I actually have a little knowledge on this. I had a tub fruit from spores. The tub had all different looking fruits I took the best looking fruit ( a non-mutant) and cloned it because the fruitbodies from the tray before were very potent. Anyways, i took the clone and expanded it. The trays from the cloned tissue produced both normal looking fruits and mutants. So in my conclusion mutants can come from any single dikaryotic organism but I have seen far more in my P.Rican tubs than any other, FAR MORE..

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Re: Stabilizing a Mutant Strain? [Re: Zen Peddler]
    #5355500 -

Isn't Penis Envy a mutent strain? I thought I read somewhere that its charicteristics were from a mutation that was stablized.


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:egyptian:
Clean flesh is next to Godflesh


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