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 Gr0wer
always improving
Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX 
Last seen: 6 years, 1 month
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 anyone ever try sterilizing a syringe without PCing? 
#3187208 - 09/27/04 04:18 PM (19 years, 6 months ago) |
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Has anyone ever sterilized a syringe with alcohol + h2o2?
I was thinking this, fill a clean syringe with alcohol let it sit for 1 min this should kill everything. Then empty and fill with 1.5% H202 this should get rid of the nasty alcohol. Now dump the h2o2 when your ready and fill with karo water. Would this work well?
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KillBill
Quick Learner
Registered: 07/02/04
Posts: 109
Last seen: 19 years, 6 months
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
#3187878 - 09/27/04 06:45 PM (19 years, 6 months ago) |
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Interesting that you should ask that, the other day i realized i would need a new sterile syringe(s), and didn't feel like pressure cooking them, so i sucked up 91% Isopropyl alcohol , spat it out, then sucked up H202 shook vigorously and spat it out. I then used liquid Inocculant on some rye jars. its been two days, a few jars so activity but here are some things that weirded me out. 1. my liquid myc has been in a fridge for a long time, maybe a month or longer and its gonna be 40 degrees in there, they did not Freeze but i feel like perhaps It did some damage to it since a lot of the jars aren't showing activity. Also when i had hte liquid inocculate in the syinge, i noticed a lot of tiny bubbles all over the place. I assume this was from H202 oxidizing(?) some of the myc but it definitely weirded me out.
More to the point though, while growth is limited in the jars i did, there are no contams present, suggesting that alcohol followed by h202 is a sufficient means of sterilizing, saving us time and gas money.
:-D Damn i feel smart, having helped someone who knows infinitely more than I.
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
#3188137 - 09/27/04 07:40 PM (19 years, 6 months ago) |
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Yep microwaved water. Draw up and purge water (right out of the microwave) several times works great!
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Psilocybeingzz
Registered: 12/15/02
Posts: 14,463
Loc: International waters
Last seen: 11 years, 5 months
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
#3188188 - 09/27/04 07:49 PM (19 years, 6 months ago) |
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I use boiled water, a'la Psilocybers syringe TEK, from the shroomery archives. 
Edited by Psilocybeingzz (09/27/04 07:50 PM)
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Cornelius
Heinous Anus
Registered: 02/12/04
Posts: 694
Loc: T.O.
Last seen: 10 years, 2 days
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
#3188252 - 09/27/04 08:03 PM (19 years, 6 months ago) |
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yeah, I boil the water for about 10 minutes then suck and purge a few times as per the syringe tek...
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 george castanza
Lord Of The Idiots!
Registered: 10/21/02
Posts: 8,759
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
#3188262 - 09/27/04 08:04 PM (19 years, 6 months ago) |
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kramer says he has not so much as tried it. 
as lazy as he is he likes the idea though! 
  
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KRAMER CAKES
  
 
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
#3188334 - 09/27/04 08:16 PM (19 years, 6 months ago) |
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The microwave gets that water so molecularly hot much hotter than boiling water could ever get, this is what makes this tek work 100% of the time. FYI guys be very careful!
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mockeylock
head
Registered: 02/07/04
Posts: 1,046
Last seen: 13 years, 8 months
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
#3188338 - 09/27/04 08:17 PM (19 years, 6 months ago) |
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It doesn't get much easier than boiling water...and it works well.
I'd keep the chemicals out of my syringes, but that's just me.
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Gr0wer
always improving
Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
Last seen: 6 years, 1 month
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Re: anyone ever try sterilizing a syringe without PCing? [Re: mockeylock]
#3188899 - 09/27/04 10:08 PM (19 years, 6 months ago) |
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thanks!
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atomic1
enthusiast
Registered: 09/18/03
Posts: 1,123
Loc: Appalachia
Last seen: 4 years, 6 months
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Re: anyone ever try sterilizing a syringe without PCing? [Re: KillBill]
#3189437 - 09/27/04 11:34 PM (19 years, 6 months ago) |
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Thats too weird you did that. Last wed. or thu. i did the exact same thing. EXACT. Rubbin alcohol 1st, i let it sit for a couple min.'s, then followed by drawing up h202. I then used some karo liquid that had been in the fridge for a few months actually several months. Anyway today, monday, i'm still yet to see ANY growth, mycelia or mold. I don't know what happend. I used some of this karo a few weeks ago and it worked fine. Now its nothing. If I was to have not sufficiently got out all the alcohol would it have killed my karo mycelia? I'm stumped. Any help would be great.
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Gr0wer
always improving
Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
Last seen: 6 years, 1 month
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Re: anyone ever try sterilizing a syringe without PCing? [Re: atomic1]
#3192275 - 09/28/04 04:56 PM (19 years, 6 months ago) |
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Just checking but do you guys still boil the syrines or just suck in/out superheated sterile water?
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