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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dj_karma_magnet]
    #29708203 -

They are growing slowly as the result of bacterial contamination. You will want to either take them to the Island or the Cabin

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 2
    #29708215 -

I missed this thread, I will post some agar here soon:rockon:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge]
    #29708282 -

Agar Wedge said:
They are growing slowly as the result of bacterial contamination. You will want to either take them to the Island or the Cabin


For sure, I will do it soon, I have cabin’d before but I am not super experienced with agar.

I have about 10 plates of lme from the same batch that have not been used, should I use these or make a new batch of plates to be safe?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dj_karma_magnet]
    #29708566 -

You can use the plates you already have. If they aren't showing any signs of contamination they're totally fine:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 3
    #29709100 -

Couple plates I sent to grain recently. Excited to see how they turn out. The PE plate is really interesting to me, haven't seen that kind of super veiny growth before.




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Re: AGAR ENVY! (Anything and All things agar!) [Re: FITMK]
    #29709144 -

Cube plin transferred to 0.75% lmea (I got my plates mixed up so it ended up on .75%). Should I take from the 6-7 area? Or the thinner 8-10 area?



And on this plate (transfer from the plate the Plin was from) where would you take from?



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Re: AGAR ENVY! (Anything and All things agar!) [Re: JoeTherapist]
    #29709157 -

On my last post on here It was suggested I cabin, I followed a technique on YouTube where the guy plops the myc down and puts the cabin over it, does not let it touch the walls.

Well I attempted to at least, was clumsy as hell and knocked the shit around, one of them I kept having to open the Petri dish again because I kept knocking the cabin off when I was parafilming the Petri dish, it happened like 5 times before I was smart enough to use my blade to slit the tape.

Another plate had a bunch of contam but none was touching the agar from what I could see, thought I’d attempt that one as well (did not add pics of that on my previous post) so I cut out the agar exact, slight issue. A drop of rubbing alcohol touched the agar, will this cause an issue?


Overall I got 3 plates and I’m really hoping at least one of them is succesful. don’t understand why I’m having such difficulty with this, my first round of agar was fine.


I also have noticed many plates I laid are uneven, and the agar all went to 1 side of the dish, I am looking for ways to prevent this, sometimes the agar is outside of the dish and inside of the foil it was in. Looking for some tips for this, it’s extremely frustrating.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JoeTherapist] * 2
    #29709165 -







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An enigma some caerulescens n a mono 😎

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bloodssonnew] * 1
    #29709582 -


May have made this trench a little big lol


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fontinalis666]
    #29709672 -

It'll all be good, make that mycelium work:mushroom2: gonna be hard for nasties to crawl across that :rockon:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 1
    #29709676 -

WORK BITCH
:badslave:
I'm storing these vertically too :awedance:

Figures that the clone I took during the same sesh that I forgot to trench is the only one with a visible baccy spot lol, guess I'll trench on the next one


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fontinalis666]
    #29709697 -

Brittney Spears would be proud:rockon::mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 7
    #29710371 -

Scemilope I couldn't help but think of you when I saw this :mushroom2:




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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 2
    #29710687 -

Back in the saddle folks:


16 plates from 400ml.  That's about my standard pour, I tend to get em a little thick.  2% agar, .625% nutes (even mix of raw honey and rye flour).  Used what I had available, and I've never had growth issues with this recipe.


-Kingboomer


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-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: AGAR ENVY! (Anything and All things agar!) [Re: kingboomer] * 4
    #29712799 -

Followed on some advice from a question on the QQQA thread about cleaning up my first ever plates. 


The plates in question-




My attempt at a cabin-


Cabin a week later




A trench and a couple of regular transfers-





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Re: AGAR ENVY! (Anything and All things agar!) [Re: Traveler42]
    #29712835 -

:botgirl:

looking great


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Learn it, know it, live it


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mackload] * 1
    #29712841 -

Besides the first photo and that tiny bacterial colony, as well the mold at 4-5 o clock on the first of the second set of photos, they look great. Getting there my guy:):mushroom2: keep up the good work!

If you haven't checked it out, the island cleanup in my signature is a much easier and more efficient way to trench, if you are pouring plates.:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 2
    #29712846 -

Maby that's my mistake with agar sandwiches I just cut a square put culture n n put back original square rather than using a bigger piece of agar to sit over top... Well at least I figured it out :laugh2:


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An enigma some caerulescens n a mono 😎

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bloodssonnew]
    #29712847 -

You got it blood, problem solved :highfive:
Sterilized cover slips work even better:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 1
    #29712879 -

Thanks! Looking forward to seeing what t3 brings.:mushroom2:

Wedge, I’ll give the island try. That does look simple!

Blood, good eye, happy cabins are in your future! This was my first, I think I defeated the purpose a bit by allowing the transfer sample to touch the roof. 

I’ve seen cabins done both fully isolated from the walls and touching the corners like I did on mine.  Is there a preferred method or just different applications?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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