Island clean up

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Agar Wedge
Hairy Cheese

Registered: 12/25/24
Posts: 2,190
Loc: Pennsylvania
Last seen: 4 minutes, 38 seconds

Island clean up

24
#29695565 - 10/12/26 04:46 PM (13 days, 16 hours ago)

Hello everybody :wave:

This is a little trick Ive been using for some time now and wanted to share. It involves pouring thin plates then dropping the wedge being transferred onto the glass( if using plastic wait a couple seconds so you don't burn a hole in the plate), it is essentially a trench, but without the extra step. This is ideal for SAB users who pour plates as it removes a step, and therefore a potential vector. I've cleaned up over a dozen varieties this way.

Step 1: Pour your plate thin so that a section isn't covered with agar, you will want to section at least the size of a nickel for ease of wedge drop. Sometimes you may pour less than expected, that's okay too. Before moving the plate into the stack wait 5-10 seconds for the agar to set, otherwise the agar will cover your island. Alternatively, you can minimize the wait by pouring the islands on the final pours, considering the agar will cool more by then. Allow the condensation to evaporate before the drop, best limit the potential of contamination to crawl with the mycelium.

Step 2: drop the wedge on its island.

Step 3: wait for the mycelium to reach "land" and then transfer to another plate. Be sure to keep the plates level to avoid accidentally transferring contamination from the transfered wedge onto the agar.

Bacteria and some other nasties have a hard time crawling across the dry surface, while mycelium has no issue doing so which is what makes this effective. Hope this helps somebody! Please let me know if you have any questions. Thanks for reading! :smile:

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Island clean up
Pasteurizing Straw with Hydrated Lime
Swiss Cheese Fruiting Chamber

Edited by Agar Wedge (10/21/26 10:14 PM)

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