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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Do Petri dishes need some sort of FAE when used for agar?
    #29673936 -

Or do the lids leak enough to let some air in?

The glass ones, to be clear.

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Thought Criminal] * 1
    #29673946 -

The air that's in there is plenty. Wrapped, with whatever you wrap with, parafilm, cling film, grafting tape, all good. Wrapped in cling film you'll get plins in a month or two.


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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: bluescreen]
    #29673958 -

I guess I’m confused about what purpose the wrapping serves if there’s enough air inside the dish? The dishes would be sealed without being wrapped, right? Or am I misunderstanding something here….

Edited by Thought Criminal (09/20/26 03:35 PM)

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Thought Criminal] * 3
    #29673961 -

To keep sterile things sterile and to prevent premature drying out.

Some of the reusable dishes I use have a threaded lid. I don't bother wrapping those


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Thought Criminal] * 1
    #29673966 -

The wrap keeps filthy bacteria and mold spore laden air from fucking up your plate.


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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: bluescreen]
    #29673971 -

That and it holds your glass plates together since they have oversized lids


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍶
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION 🧪
JOIN CAKE WARS III TODAY 🫵
MODERN FRUITING CONDITIONS 🍄
REVITALIZE THE CUBENSIS GENEPOOL 🥊
MY FAVORITE TEKS 📖

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: langten69]
    #29673976 -

Ohhhh, I was under the impression that they fit snugly on their own.

Now I understand!

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: PuffM4gicDr4gon]
    #29673978 -

PuffM4gicDr4gon said:
Some of the reusable dishes I use have a threaded lid. I don't bother wrapping those


I’m starting to wonder if the little 4oz mason jars might be the easiest option for agar….

Being able to pour the agar while cool then PC sure sounds like it would make life easier, even if the lid isn’t clear.

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Thought Criminal] * 1
    #29673985 -

Thought Criminal said:
PuffM4gicDr4gon said:
Some of the reusable dishes I use have a threaded lid. I don't bother wrapping those


I’m starting to wonder if the little 4oz mason jars might be the easiest option for agar….

Being able to pour the agar while cool then PC sure sounds like it would make life easier, even if the lid isn’t clear.


Pouring hot agar onto regular petri dishes sounds more simple and more space efficient to me atleast. having 10 plates stacked in your workspace is better than 10 jars i believe :sunny:

Best of luck


--------------------
I wish you bountiful fruits and godspeed :heart:

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Mashedpotatoes] * 1
    #29673986 -

I use these mostly, although I do have some glass and single-use petri dishes as well.

https://www.shroomery.org/forums/showflat.php/Number/26374264#26374264


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: PuffM4gicDr4gon]
    #29673989 -

Nice, thanks for posting this. Im seeing pins on a plate I nocd up on 8/31 and I was wondering if there was enough air in there for it to finish maturing at least into a big pin I could close.

The first time I did agar dishes, i wrapped them around the top and bottom instead of around the sides 🤦‍♀️ one by one my plates all got contaminated until one night my eyes popped open like the squidward meme as I realized my mistake


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idrk how I got here or what I'm here for but I  keep waking up with this biological urge to not die✨

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: skrimplet] * 2
    #29673991 -

skrimplet said:
Nice, thanks for posting this. Im seeing pins on a plate I nocd up on 8/31 and I was wondering if there was enough air in there for it to finish maturing at least into a big pin I could close.

The first time I did agar dishes, i wrapped them around the top and bottom instead of around the sides 🤦‍♀️ one by one my plates all got contaminated until one night my eyes popped open like the squidward meme as I realized my mistake


I swab gills on mature plate fruits for ulta clean spores.

And I'm team cling film.


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Edited by bluescreen (09/20/26 04:06 PM)

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: PuffM4gicDr4gon]
    #29673994 -

PuffM4gicDr4gon said:
https://www.shroomery.org/forums/showflat.php/Number/26374264#26374264


So that plastic is strong enough to hold up to repeated PC’ing?

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: bluescreen] * 2
    #29673996 -

Quote:
I swab gills on mature plate fruits for ulta clean spores.

And I'm team cling film.



wow, i'd have never thought of that myself:takingnotes::takingnotes:


--------------------
I wish you bountiful fruits and godspeed :heart:

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Thought Criminal]
    #29674006 -

Thought Criminal said:
PuffM4gicDr4gon said:
https://www.shroomery.org/forums/showflat.php/Number/26374264#26374264


So that plastic is strong enough to hold up to repeated PC’ing?


You want PP5 plastic. That's the stuff that can handle a PC cycle.

Nichrome says that some bacteria can make it through PP5 after multiple PC cycles. Specifically Psuedomonas. The plastic on my plates started becoming discolored after about a year, so I replaced them. I have yet to see proof of bacteria bypassing the plastic on my plate, but they're cheap enough that I don't mind replacing them every now and again.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: PuffM4gicDr4gon]
    #29674017 -

PuffM4gicDr4gon said:
Thought Criminal said:
PuffM4gicDr4gon said:
https://www.shroomery.org/forums/showflat.php/Number/26374264#26374264


So that plastic is strong enough to hold up to repeated PC’ing?


You want PP5 plastic. That's the stuff that can handle a PC cycle.

Nichrome says that some bacteria can make it through PP5 after multiple PC cycles. Specifically Psuedomonas. The plastic on my plates started becoming discolored after about a year, so I replaced them. I have yet to see proof of bacteria bypassing the plastic on my plate, but they're cheap enough that I don't mind replacing them every now and again.


I recall Faht had bacteria issues he tracked down to reusable plates.


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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: PuffM4gicDr4gon]
    #29674018 -

PuffM4gicDr4gon said:
Thought Criminal said:
PuffM4gicDr4gon said:
https://www.shroomery.org/forums/showflat.php/Number/26374264#26374264


So that plastic is strong enough to hold up to repeated PC’ing?


You want PP5 plastic. That's the stuff that can handle a PC cycle.

Nichrome says that some bacteria can make it through PP5 after multiple PC cycles. Specifically Psuedomonas. The plastic on my plates started becoming discolored after about a year, so I replaced them. I have yet to see proof of bacteria bypassing the plastic on my plate, but they're cheap enough that I don't mind replacing them every now and again.


Cool, thanks.

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Thought Criminal]
    #29674058 -

Thought Criminal said:
PuffM4gicDr4gon said:
Some of the reusable dishes I use have a threaded lid. I don't bother wrapping those


I’m starting to wonder if the little 4oz mason jars might be the easiest option for agar….

Being able to pour the agar while cool then PC sure sounds like it would make life easier, even if the lid isn’t clear.


I use the mason jars too
I give them a tiny fae hole and put micropore over it


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍶
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION 🧪
JOIN CAKE WARS III TODAY 🫵
MODERN FRUITING CONDITIONS 🍄
REVITALIZE THE CUBENSIS GENEPOOL 🥊
MY FAVORITE TEKS 📖

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Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Mashedpotatoes] * 1
    #29674059 -

Mashedpotatoes said:
Quote:
I swab gills on mature plate fruits for ulta clean spores.

And I'm team cling film.



wow, i'd have never thought of that myself:takingnotes::takingnotes:


:mindexpanding:


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍶
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION 🧪
JOIN CAKE WARS III TODAY 🫵
MODERN FRUITING CONDITIONS 🍄
REVITALIZE THE CUBENSIS GENEPOOL 🥊
MY FAVORITE TEKS 📖

Extras: Filter Print Post Top
Re: Do Petri dishes need some sort of FAE when used for agar? [Re: Mashedpotatoes] * 1
    #29674141 -

Mashedpotatoes said:
Quote:
I swab gills on mature plate fruits for ulta clean spores.

And I'm team cling film.



wow, i'd have never thought of that myself:takingnotes::takingnotes:


Of course the intelligent grower does BRF pucks too, with cubes almost a given to get pins and fruits, clean af.:toast:

Throw pins right on to agar, or whatever, they're cleeeean.


--------------------

Edited by bluescreen (09/20/26 07:08 PM)

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