Re: Petri Dishes & Agar Q's - Mushroom Cultivation

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Placebo
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Registered: 12/15/98
Posts: 55
Last seen: 23 years, 9 months

Re: Petri Dishes & Agar Q's

#67265 - 12/02/99 12:09 AM (25 years, 4 months ago)

I use and reuse plastic "disposable" petris. I wash and clean them thoroughly, sometimes with some sort of disinfectant, and wipe them down with alcohol and let them dry (in a glove box) before using them. I sometimes get some spots of contamination that pop up (which are probably due to improperly cleaning the petris), but it's usually pretty rare. Just don't try sterilizing them in a pressure cooker. :smile:

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Placebo
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Registered: 12/15/98
Posts: 55
Last seen: 23 years, 9 months

Re: Petri Dishes & Agar Q's

[Re: Placebo]
#67267 - 12/02/99 12:23 AM (25 years, 4 months ago)

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PanTrop
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Registered: 11/02/99
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Re: Petri Dishes & Agar Q's

[Re: Placebo]
#67268 - 12/03/99 04:53 PM (25 years, 4 months ago)

If the petri dishes were open there not steril anymore....you can still use them, but since there plastic they will not survive a pressure cooker, try bringing water to a soft boil and let the dishes sit in it for about 30 minutes....This should be good and then to be really safe wipe the dishes down with a paper towel soaked in rubbing alcohol. As for reusing them after you have used them....I wouldnt but you could repeat the steps above I guess with petri dishes that didnt have contams.

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PermaGrin
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Registered: 01/31/99
Posts: 30

Re: Petri Dishes & Agar Q's

[Re: Placebo]
#67269 - 12/04/99 08:50 AM (25 years, 4 months ago)

Has anyone here actually tried to sterilize these plastic petris in a pressure cooker? I recently cooked a plastic disposable syringe with some salsa I was canning, and it held up to the heat just fine.

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Pipes of Pan
journeyman

Registered: 08/16/99
Posts: 18
Last seen: 1 year, 4 months

Re: Petri Dishes & Agar Q's

[Re: Placebo]
#67270 - 12/04/99 12:32 PM (25 years, 4 months ago)

There are plastic petri dishes that can be sterilized in the pressure cooker, but not the styrene disposable ones. I speak from experience here. If you are into plastic free form sculpture, try pressure cooking these styrene petri dishes. That is exactly what you will get and it is a bit hard to pour agar into something like that. I do resuse mine too, but it is getting to be more a pain in the ass. They get all scratched up and the scratches can harbor contams. I soak mine in a diluted bleach solution overnight and wash the hell out of them with fresh water then.

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Anonymous

Re: Petri Dishes & Agar Q's

[Re: Placebo]
#67271 - 12/07/99 10:00 AM (25 years, 4 months ago)

Yeah, I tried sterilizing mine and all I got was one big pile of melted plastic!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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