Home | Community | Message Board



Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Autoclave, Laminar Flow Hood, Petri Dish, Pressure Cooker

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
Offlinesparafucile
Stranger
Registered: 07/12/01
Posts: 9
Last seen: 14 years, 10 months
Steralizing Plastic Petri Dishes
    #620601 - 04/27/02 06:28 PM (14 years, 10 months ago)

Hi. I'm wondering, I've got some standard plastic petri dishes that I've already used. Therefore, I would like to steralize them in my pressure cooker. Is this possible? It doesn't seem like they would break, and other than keeping them from touching the sides while cooking, there shouldn't be any other problem, but I wanted to make sure. Does anyone know of any other good ways to steralize some dishes right before I drop some agar in them (lysol?)? Thanks for your time.

-recovering from contam :wink: 


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefrancisco
Richman Sporeman
Registered: 01/15/02
Posts: 133
Loc: USA
Last seen: 13 years, 9 months
Re: Steralizing Plastic Petri Dishes [Re: sparafucile]
    #620669 - 04/27/02 08:34 PM (14 years, 10 months ago)

They get deformed by the pressure/heat?i clean in a antimicrobial detergent and let dry in still air.Use 91% alcohol(walmart) to submerge the parts for a few seconds.Stack in your glove box and let dry.DANGER alcohol fumes will ignite in the present of any spart.even static charge so use caution when using.No smoking No open flames.


--------------------
Well...Maybe just a little.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleshaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
Re: Steralizing Plastic Petri Dishes [Re: francisco]
    #620670 - 04/27/02 08:39 PM (14 years, 10 months ago)

you can use Hydrogen peroxide .


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefrancisco
Richman Sporeman
Registered: 01/15/02
Posts: 133
Loc: USA
Last seen: 13 years, 9 months
Re: Steralizing Plastic Petri Dishes [Re: shaggymane]
    #620716 - 04/27/02 09:39 PM (14 years, 10 months ago)

i lose a few with h2o2 but it is safer


--------------------
Well...Maybe just a little.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineshubrick
Male User Gallery


Registered: 06/11/10
Posts: 145
Last seen: 1 month, 7 days
Re: Steralizing Plastic Petri Dishes [Re: francisco]
    #12868288 - 07/08/10 06:08 PM (6 years, 8 months ago)

Quote:

francisco said:
i lose a few with h2o2 but it is safer 




wait, why would you lose a few using h2o2?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblejokefox
Top of the chain
Male User Gallery


Registered: 12/22/09
Posts: 6,216
Loc: never where I should be
Re: Steralizing Plastic Petri Dishes [Re: shubrick]
    #12868306 - 07/08/10 06:12 PM (6 years, 8 months ago)

cant you do syringes in the pressure cooker if you wrap them in tinfoil

whats the purpose of the tin foil.. to act as a package?

i would Imagen the plastic is the same quality in the petris as the syringes
so in theory shouldent you be able to wrap tinfoil around the petri dishes and pressure cook

i have some zip ties that are only rated to 185F and they withstand the pressure cooker



just noticed.... 8 yr old thread
this is zombie shit now :facepalm:


Edited by jokefox (07/08/10 06:13 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinenewbie_shroomer
Stranger
Registered: 02/20/07
Posts: 185
Last seen: 3 years, 6 months
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868416 - 07/08/10 06:28 PM (6 years, 8 months ago)

Well if petri dishes deform then they may be a different type of plastic to the syringes. I have been told to use P5 plastic for pressure cooking but i have found that my p5 jars are quite thin and became deformed but the thicker ones where fine, so it may be a case f the plastics thickness.

The tinfoil would be used as a heat sink, so that your syringe is not touching the side of the PC directly causing it to melt? Well thats my guess anyway.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblejokefox
Top of the chain
Male User Gallery


Registered: 12/22/09
Posts: 6,216
Loc: never where I should be
Re: Steralizing Plastic Petri Dishes [Re: newbie_shroomer]
    #12868442 - 07/08/10 06:32 PM (6 years, 8 months ago)

so then in theory plastic petri dishes should hold up...

im going to try this later today , when im not to stoned to dump out contams

check back in a few hours for a result


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinebaron_samedi
Urban Naturalist Industries


Registered: 04/07/04
Posts: 1,650
Loc: HI Flag
Last seen: 17 hours, 6 minutes
Re: Steralizing Plastic Petri Dishes [Re: newbie_shroomer]
    #12868520 - 07/08/10 06:43 PM (6 years, 8 months ago)

i was wondering the same thing i dont have the dishes yet but that is gonna be my first experiment plastic petris in an autoclave and i know for a fact that most lure lok syringes are autoclaveable without foil and they dont melt at all but i also putin autoclave bags so im not sure if that helps (thats how i do my syringes before i fill with sterile water). keep me posted


--------------------
On Deck For Trade...
Trade List
:acidfire::deathrush::acidfire::myfaceismelting::acidfire:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefungus_tao
Hah Zah!
Male User Gallery


Registered: 05/10/06
Posts: 1,856
Loc: Flag
Last seen: 25 days, 11 hours
Re: Steralizing Plastic Petri Dishes [Re: baron_samedi]
    #12868530 - 07/08/10 06:45 PM (6 years, 8 months ago)

Are you guys talking about disposable plastic dishes? They melt.
Tao


--------------------
Follow the light
The Light is your guide.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehamloaf
Mushrooms are Tight.
Male User Gallery

Registered: 12/23/09
Posts: 13,256
Loc: Alder House Mushrooms. Flag
Last seen: 16 hours, 57 minutes
Trusted Cultivator
Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
    #12868545 - 07/08/10 06:47 PM (6 years, 8 months ago)

I have been thinking, to sterilize plastic petri dishes.
How about a 70% alcohol bath.

Then dried in front of a flow hood.
I have yet to try this.
HL


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblejokefox
Top of the chain
Male User Gallery


Registered: 12/22/09
Posts: 6,216
Loc: never where I should be
Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
    #12868555 - 07/08/10 06:49 PM (6 years, 8 months ago)

they melt?
at what pressure

im going to try 12 psi for 15 minutes


bath with 97% iso


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefungus_tao
Hah Zah!
Male User Gallery


Registered: 05/10/06
Posts: 1,856
Loc: Flag
Last seen: 25 days, 11 hours
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868575 - 07/08/10 06:52 PM (6 years, 8 months ago)

Something like this would be better.
Tao


--------------------
Follow the light
The Light is your guide.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleM11
White Thumb


Registered: 06/14/09
Posts: 1,840
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868590 - 07/08/10 06:54 PM (6 years, 8 months ago)

I think you should pressure cook them at 20 psi using 91% alcohol instead of water...

:tongue:

I would think PCing them at 12 psi for 20 minutes would be fine.  I guess they could melt?  I would wrap them in aluminum foil -not too tightly, but just enough.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblejokefox
Top of the chain
Male User Gallery


Registered: 12/22/09
Posts: 6,216
Loc: never where I should be
Re: Steralizing Plastic Petri Dishes [Re: M11]
    #12868603 - 07/08/10 06:57 PM (6 years, 8 months ago)

have you ever added hot water to 97%iso

ya i did in a jar then put the disk over and shook it
and it exploded hot water all over my arms
i was shaking for 20 minutes after,, i needed a fat toke and a smoke to calm down
my arms were all burnt from the water to ... bad day

dont fuck with hot water and iso


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblejokefox
Top of the chain
Male User Gallery


Registered: 12/22/09
Posts: 6,216
Loc: never where I should be
Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
    #12868605 - 07/08/10 06:57 PM (6 years, 8 months ago)

Quote:

fungus_tao said:
Something like this would be better.
Tao





i heard this doesent work


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleNuminosum
President of Turd Town
Male User Gallery Arcade Champion: Snake


Registered: 05/19/09
Posts: 1,175
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868647 - 07/08/10 07:05 PM (6 years, 8 months ago)

Double post


--------------------
...within my memory is the knowledge of hyper-light drive ships and how to build them.



Doc_T's Efficiency Challenge


Edited by Numinosum (07/08/10 07:06 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleNuminosum
President of Turd Town
Male User Gallery Arcade Champion: Snake


Registered: 05/19/09
Posts: 1,175
Re: Steralizing Plastic Petri Dishes [Re: Numinosum]
    #12868652 - 07/08/10 07:05 PM (6 years, 8 months ago)

Quote:

Numinosum said:
Quote:

jokefox said:
Quote:

fungus_tao said:
Something like this would be better.
Tao





i heard this doesent work




My wife bought one of those, I laughed. The thing costs like $40 dollars. Germicidal lamps
used in labs cost hundreds yes?  Anyway, when I read the instructions it tells you how long to
keep the light over the surface, I forget how long now but I do remember it was a little
ridiculous.




--------------------
...within my memory is the knowledge of hyper-light drive ships and how to build them.



Doc_T's Efficiency Challenge


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefungus_tao
Hah Zah!
Male User Gallery


Registered: 05/10/06
Posts: 1,856
Loc: Flag
Last seen: 25 days, 11 hours
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868690 - 07/08/10 07:09 PM (6 years, 8 months ago)

UVC is the same thing used in the bacti cinerator.

From wikipedia:
Quote:

Ultraviolet lamps are used to sterilize workspaces and tools used in biology laboratories and medical facilities.





Tao


--------------------
Follow the light
The Light is your guide.


Edited by fungus_tao (07/08/10 07:14 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefungus_tao
Hah Zah!
Male User Gallery


Registered: 05/10/06
Posts: 1,856
Loc: Flag
Last seen: 25 days, 11 hours
Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
    #12868705 - 07/08/10 07:13 PM (6 years, 8 months ago)

Quote:

Anyway, when I read the instructions it tells you how long to
keep the light over the surface, I forget how long now but I do remember it was a little
ridiculous.




I can see why the home use ones would be numbed down a bit so people don't give themselves skin cancer and sue. Maybe these types aren't as effective :shrug:

Jokefox, are you PC'ing plastic dishes?

Tao


--------------------
Follow the light
The Light is your guide.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Amazon Shop for: ½ Pint Jars, Agar, Autoclave, Laminar Flow Hood, Petri Dish, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Sterilizing Plastic Petri Dishes drainhaven 1,950 14 06/01/09 02:46 AM
by Mykologist
* question about plastic petri dishes Duffmanooohyea 1,440 14 01/08/05 03:42 PM
by Prisoner#1
* Plastic petri dishes in PC? HUBSonDUBS 840 2 02/07/07 06:20 PM
by RogerRabbit
* plastic petri dish's cheesie 908 9 03/11/07 02:21 PM
by cheesie
* reusing plastic petri dishes blood4blood 707 2 02/25/08 01:32 PM
by blood4blood
* How to Clean and Reuse Plastic Petri Dishes? WhAcKeD 1,289 4 06/07/07 01:26 PM
by figgusfiddus
* pouring petri dishes in a glovebox.
( 1 2 all )
tripchip 6,361 24 09/22/08 07:09 AM
by tripchip
* Reusing petri dishes? SubGen1us 1,305 8 10/28/08 07:14 PM
by FooMan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart, Tmethyl
5,986 topic views. 12 members, 55 guests and 35 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Zamnesia.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.046 seconds spending 0.003 seconds on 16 queries.