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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Steralizing Plastic Petri Dishes
    #620601 -

Hi. I'm wondering, I've got some standard plastic petri dishes that I've already used. Therefore, I would like to steralize them in my pressure cooker. Is this possible? It doesn't seem like they would break, and other than keeping them from touching the sides while cooking, there shouldn't be any other problem, but I wanted to make sure. Does anyone know of any other good ways to steralize some dishes right before I drop some agar in them (lysol?)? Thanks for your time.

-recovering from contam :wink: 

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Re: Steralizing Plastic Petri Dishes [Re: sparafucile]
    #620669 -

They get deformed by the pressure/heat?i clean in a antimicrobial detergent and let dry in still air.Use 91% alcohol(walmart) to submerge the parts for a few seconds.Stack in your glove box and let dry.DANGER alcohol fumes will ignite in the present of any spart.even static charge so use caution when using.No smoking No open flames.


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Well...Maybe just a little.

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Re: Steralizing Plastic Petri Dishes [Re: francisco]
    #620670 -

you can use Hydrogen peroxide .

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Re: Steralizing Plastic Petri Dishes [Re: shaggymane]
    #620716 -

i lose a few with h2o2 but it is safer


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Well...Maybe just a little.

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Re: Steralizing Plastic Petri Dishes [Re: francisco]
    #12868288 -

francisco said:
i lose a few with h2o2 but it is safer 


wait, why would you lose a few using h2o2?

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Re: Steralizing Plastic Petri Dishes [Re: shubrick]
    #12868306 -

cant you do syringes in the pressure cooker if you wrap them in tinfoil

whats the purpose of the tin foil.. to act as a package?

i would Imagen the plastic is the same quality in the petris as the syringes
so in theory shouldent you be able to wrap tinfoil around the petri dishes and pressure cook

i have some zip ties that are only rated to 185F and they withstand the pressure cooker



just noticed.... 8 yr old thread
this is zombie shit now :facepalm:


--------------------
Spore Whore
A clear conscience is usually the sign of a bad memory.
How to: the heart to automation (clicky)
How to: jokefox's agar broth (replace your LC with something that works)
How to: jokefox's MMS
Indoor complete Outdoor in progress

Edited by jokefox (07/08/10 04:13 PM)

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Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868416 -

Well if petri dishes deform then they may be a different type of plastic to the syringes. I have been told to use P5 plastic for pressure cooking but i have found that my p5 jars are quite thin and became deformed but the thicker ones where fine, so it may be a case f the plastics thickness.

The tinfoil would be used as a heat sink, so that your syringe is not touching the side of the PC directly causing it to melt? Well thats my guess anyway.

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Re: Steralizing Plastic Petri Dishes [Re: newbie_shroomer]
    #12868442 -

so then in theory plastic petri dishes should hold up...

im going to try this later today , when im not to stoned to dump out contams

check back in a few hours for a result


--------------------
Spore Whore
A clear conscience is usually the sign of a bad memory.
How to: the heart to automation (clicky)
How to: jokefox's agar broth (replace your LC with something that works)
How to: jokefox's MMS
Indoor complete Outdoor in progress

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Re: Steralizing Plastic Petri Dishes [Re: newbie_shroomer]
    #12868520 -

i was wondering the same thing i dont have the dishes yet but that is gonna be my first experiment plastic petris in an autoclave and i know for a fact that most lure lok syringes are autoclaveable without foil and they dont melt at all but i also putin autoclave bags so im not sure if that helps (thats how i do my syringes before i fill with sterile water). keep me posted


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:acidfire::deathrush::acidfire::myfaceismelting::acidfire:

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Re: Steralizing Plastic Petri Dishes [Re: baron_samedi]
    #12868530 -

Are you guys talking about disposable plastic dishes? They melt.
Tao


--------------------
Follow the light
The Light is your guide.

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Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
    #12868545 -

I have been thinking, to sterilize plastic petri dishes.
How about a 70% alcohol bath.

Then dried in front of a flow hood.
I have yet to try this.
HL


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
    #12868555 -

they melt?
at what pressure

im going to try 12 psi for 15 minutes


bath with 97% iso


--------------------
Spore Whore
A clear conscience is usually the sign of a bad memory.
How to: the heart to automation (clicky)
How to: jokefox's agar broth (replace your LC with something that works)
How to: jokefox's MMS
Indoor complete Outdoor in progress

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Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868575 -

Something like this would be better.
Tao


--------------------
Follow the light
The Light is your guide.

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Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868590 -

I think you should pressure cook them at 20 psi using 91% alcohol instead of water...

:tongue:

I would think PCing them at 12 psi for 20 minutes would be fine.  I guess they could melt?  I would wrap them in aluminum foil -not too tightly, but just enough.

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Re: Steralizing Plastic Petri Dishes [Re: M11]
    #12868603 -

have you ever added hot water to 97%iso

ya i did in a jar then put the disk over and shook it
and it exploded hot water all over my arms
i was shaking for 20 minutes after,, i needed a fat toke and a smoke to calm down
my arms were all burnt from the water to ... bad day

dont fuck with hot water and iso


--------------------
Spore Whore
A clear conscience is usually the sign of a bad memory.
How to: the heart to automation (clicky)
How to: jokefox's agar broth (replace your LC with something that works)
How to: jokefox's MMS
Indoor complete Outdoor in progress

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Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
    #12868605 -

fungus_tao said:
Something like this would be better.
Tao



i heard this doesent work


--------------------
Spore Whore
A clear conscience is usually the sign of a bad memory.
How to: the heart to automation (clicky)
How to: jokefox's agar broth (replace your LC with something that works)
How to: jokefox's MMS
Indoor complete Outdoor in progress

Extras: Filter Print Post Top
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868647 -

Double post


--------------------
...within my memory is the knowledge of hyper-light drive ships and how to build them.



Doc_T's Efficiency Challenge

Edited by Numinosum (07/08/10 05:06 PM)

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Re: Steralizing Plastic Petri Dishes [Re: Numinosum]
    #12868652 -

Numinosum said:
jokefox said:
fungus_tao said:
Something like this would be better.
Tao



i heard this doesent work


My wife bought one of those, I laughed. The thing costs like $40 dollars. Germicidal lamps
used in labs cost hundreds yes?  Anyway, when I read the instructions it tells you how long to
keep the light over the surface, I forget how long now but I do remember it was a little
ridiculous.



--------------------
...within my memory is the knowledge of hyper-light drive ships and how to build them.



Doc_T's Efficiency Challenge

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Re: Steralizing Plastic Petri Dishes [Re: jokefox]
    #12868690 -

UVC is the same thing used in the bacti cinerator.

From wikipedia:
Quote:
Ultraviolet lamps are used to sterilize workspaces and tools used in biology laboratories and medical facilities.




Tao


--------------------
Follow the light
The Light is your guide.

Edited by fungus_tao (07/08/10 05:14 PM)

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Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
    #12868705 -

Quote:
Anyway, when I read the instructions it tells you how long to
keep the light over the surface, I forget how long now but I do remember it was a little
ridiculous.


I can see why the home use ones would be numbed down a bit so people don't give themselves skin cancer and sue. Maybe these types aren't as effective :shrug:

Jokefox, are you PC'ing plastic dishes?

Tao


--------------------
Follow the light
The Light is your guide.

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