|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
sparafucile
Stranger
Registered: 07/12/01
Posts: 9
Last seen: 22 years, 9 months
|
Steralizing Plastic Petri Dishes
#620601 - 04/27/02 04:28 PM (22 years, 9 months ago) |
|
|
Hi. I'm wondering, I've got some standard plastic petri dishes that I've already used. Therefore, I would like to steralize them in my pressure cooker. Is this possible? It doesn't seem like they would break, and other than keeping them from touching the sides while cooking, there shouldn't be any other problem, but I wanted to make sure. Does anyone know of any other good ways to steralize some dishes right before I drop some agar in them (lysol?)? Thanks for your time.
-recovering from contam
|
francisco
Richman Sporeman
Registered: 01/15/02
Posts: 133
Loc: USA
Last seen: 6 years, 6 months
|
Re: Steralizing Plastic Petri Dishes [Re: sparafucile]
#620669 - 04/27/02 06:34 PM (22 years, 9 months ago) |
|
|
They get deformed by the pressure/heat?i clean in a antimicrobial detergent and let dry in still air.Use 91% alcohol(walmart) to submerge the parts for a few seconds.Stack in your glove box and let dry.DANGER alcohol fumes will ignite in the present of any spart.even static charge so use caution when using.No smoking No open flames.
-------------------- Well...Maybe just a little.
|
shaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
|
Re: Steralizing Plastic Petri Dishes [Re: francisco]
#620670 - 04/27/02 06:39 PM (22 years, 9 months ago) |
|
|
you can use Hydrogen peroxide .
|
francisco
Richman Sporeman
Registered: 01/15/02
Posts: 133
Loc: USA
Last seen: 6 years, 6 months
|
Re: Steralizing Plastic Petri Dishes [Re: shaggymane]
#620716 - 04/27/02 07:39 PM (22 years, 9 months ago) |
|
|
i lose a few with h2o2 but it is safer
-------------------- Well...Maybe just a little.
|
shubrick



Registered: 06/11/10
Posts: 145
Last seen: 3 years, 11 months
|
Re: Steralizing Plastic Petri Dishes [Re: francisco]
#12868288 - 07/08/10 04:08 PM (14 years, 7 months ago) |
|
|
Quote:
francisco said: i lose a few with h2o2 but it is safer
wait, why would you lose a few using h2o2?
|
jokefox
Top of the chain



Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
|
Re: Steralizing Plastic Petri Dishes [Re: shubrick]
#12868306 - 07/08/10 04:12 PM (14 years, 7 months ago) |
|
|
cant you do syringes in the pressure cooker if you wrap them in tinfoil
whats the purpose of the tin foil.. to act as a package?
i would Imagen the plastic is the same quality in the petris as the syringes so in theory shouldent you be able to wrap tinfoil around the petri dishes and pressure cook i have some zip ties that are only rated to 185F and they withstand the pressure cooker
just noticed.... 8 yr old thread this is zombie shit now
Edited by jokefox (07/08/10 04:13 PM)
|
newbie_shroomer
Stranger
Registered: 02/20/07
Posts: 185
Last seen: 11 years, 5 months
|
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
#12868416 - 07/08/10 04:28 PM (14 years, 7 months ago) |
|
|
Well if petri dishes deform then they may be a different type of plastic to the syringes. I have been told to use P5 plastic for pressure cooking but i have found that my p5 jars are quite thin and became deformed but the thicker ones where fine, so it may be a case f the plastics thickness.
The tinfoil would be used as a heat sink, so that your syringe is not touching the side of the PC directly causing it to melt? Well thats my guess anyway.
|
jokefox
Top of the chain



Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
|
|
so then in theory plastic petri dishes should hold up...
im going to try this later today , when im not to stoned to dump out contams
check back in a few hours for a result
|
baron_samedi
Ancient Shroomerite


Registered: 04/07/04
Posts: 1,700
Loc: HI
Last seen: 1 year, 18 days
|
|
i was wondering the same thing i dont have the dishes yet but that is gonna be my first experiment plastic petris in an autoclave and i know for a fact that most lure lok syringes are autoclaveable without foil and they dont melt at all but i also putin autoclave bags so im not sure if that helps (thats how i do my syringes before i fill with sterile water). keep me posted
|
fungus_tao
Hah Zah!



Registered: 05/10/06
Posts: 1,856
Loc:
Last seen: 7 years, 4 months
|
Re: Steralizing Plastic Petri Dishes [Re: baron_samedi]
#12868530 - 07/08/10 04:45 PM (14 years, 7 months ago) |
|
|
Are you guys talking about disposable plastic dishes? They melt. Tao
-------------------- Follow the light
The Light is your guide.
|
hamloaf
Pork block ®©™√


Registered: 12/23/09
Posts: 24,447
Loc: ation: Undisclosed
Last seen: 4 hours, 6 minutes
|
Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
#12868545 - 07/08/10 04:47 PM (14 years, 7 months ago) |
|
|
I have been thinking, to sterilize plastic petri dishes. How about a 70% alcohol bath.
Then dried in front of a flow hood. I have yet to try this. HL
|
jokefox
Top of the chain



Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
|
Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
#12868555 - 07/08/10 04:49 PM (14 years, 7 months ago) |
|
|
they melt? at what pressure
im going to try 12 psi for 15 minutes
bath with 97% iso
|
fungus_tao
Hah Zah!



Registered: 05/10/06
Posts: 1,856
Loc:
Last seen: 7 years, 4 months
|
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
#12868575 - 07/08/10 04:52 PM (14 years, 7 months ago) |
|
|
Something like this would be better. Tao
-------------------- Follow the light
The Light is your guide.
|
M11
White Thumb


Registered: 06/14/09
Posts: 1,840
|
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
#12868590 - 07/08/10 04:54 PM (14 years, 7 months ago) |
|
|
I think you should pressure cook them at 20 psi using 91% alcohol instead of water...

I would think PCing them at 12 psi for 20 minutes would be fine. I guess they could melt? I would wrap them in aluminum foil -not too tightly, but just enough.
|
jokefox
Top of the chain



Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
|
Re: Steralizing Plastic Petri Dishes [Re: M11]
#12868603 - 07/08/10 04:57 PM (14 years, 7 months ago) |
|
|
have you ever added hot water to 97%iso
ya i did in a jar then put the disk over and shook it and it exploded hot water all over my arms i was shaking for 20 minutes after,, i needed a fat toke and a smoke to calm down my arms were all burnt from the water to ... bad day
dont fuck with hot water and iso
|
jokefox
Top of the chain



Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
|
Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
#12868605 - 07/08/10 04:57 PM (14 years, 7 months ago) |
|
|
Quote:
fungus_tao said: Something like this would be better. Tao
i heard this doesent work
|
Numinosum
President of Turd Town



Registered: 05/19/09
Posts: 1,175
|
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
#12868647 - 07/08/10 05:05 PM (14 years, 7 months ago) |
|
|
Double post
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them.
Doc_T's Efficiency Challenge
Edited by Numinosum (07/08/10 05:06 PM)
|
Numinosum
President of Turd Town



Registered: 05/19/09
Posts: 1,175
|
Re: Steralizing Plastic Petri Dishes [Re: Numinosum]
#12868652 - 07/08/10 05:05 PM (14 years, 7 months ago) |
|
|
Quote:
Numinosum said:
Quote:
jokefox said:
Quote:
fungus_tao said: Something like this would be better. Tao
i heard this doesent work
My wife bought one of those, I laughed. The thing costs like $40 dollars. Germicidal lamps used in labs cost hundreds yes? Anyway, when I read the instructions it tells you how long to keep the light over the surface, I forget how long now but I do remember it was a little ridiculous.
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them.
Doc_T's Efficiency Challenge
|
fungus_tao
Hah Zah!



Registered: 05/10/06
Posts: 1,856
Loc:
Last seen: 7 years, 4 months
|
Re: Steralizing Plastic Petri Dishes [Re: jokefox]
#12868690 - 07/08/10 05:09 PM (14 years, 7 months ago) |
|
|
UVC is the same thing used in the bacti cinerator.
From wikipedia:
Quote:
Ultraviolet lamps are used to sterilize workspaces and tools used in biology laboratories and medical facilities.
Tao
-------------------- Follow the light
The Light is your guide.
Edited by fungus_tao (07/08/10 05:14 PM)
|
fungus_tao
Hah Zah!



Registered: 05/10/06
Posts: 1,856
Loc:
Last seen: 7 years, 4 months
|
Re: Steralizing Plastic Petri Dishes [Re: fungus_tao]
#12868705 - 07/08/10 05:13 PM (14 years, 7 months ago) |
|
|
Quote:
Anyway, when I read the instructions it tells you how long to keep the light over the surface, I forget how long now but I do remember it was a little ridiculous.
I can see why the home use ones would be numbed down a bit so people don't give themselves skin cancer and sue. Maybe these types aren't as effective 
Jokefox, are you PC'ing plastic dishes?
Tao
-------------------- Follow the light
The Light is your guide.
|
|