RockinRobot said:

What issues?

I personally told you the way you make your spore syringes is bunk. Several members told you you aren't sterilizing your jars enough or correctly. You can get away with steaming PF-TEK without a PC but it takes much longer. Several also commented on your agar including myself.

You threw every comment back in someones face. Starting to think you belong on Reddit instead of here. They will tell you whatever you want to hear over there.

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PuffM4gicDr4gon said:

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PuffM4gicDr4gon said:

OK, this has gotten ridiculous.

1.Updated PF-Tek Guide 2023 Edition specifically lists a steam time of 90-120 minutes. Op says he does 120. Why should him buying new equipment be a prerequisite for receiving help when he's literally using an approved method for PF Tek sterilization?

2. Stipe says boiling water is sufficient for sterilizing syringes OP may or may not be sterilizing the container they mix their spores in, but no one has directly asked. Ya'll just assume he's wrong

3. Rev's BRF Tek specifically calls for using a filter and agar, and lists one of the potential uses as dealing with contamination. Not one of you mentioned or gave advice about using this to clean up a culture. BRF Puck to Water Agar CS cleaned up a bacterial AF LC for me. This will get rid of all but the most stubborn bacteria. The only difference is he uses MP tape instead of a SFD, but MP tape can be used for Grain if you layer it. It's old info, but still works. If it didn't work his pucks would trich out.

4. OP has had success for multiple years and the problem is recent. While it is possible laziness has creeped into his process, but it's far more likely that he has some kind of unknown contamination in his spore print. BRF is resistant to bacteria, but it's not fool proof. Some bacteria can move on it's own. If he has that, then no transfers, BRF Pucks, teks, etc... will work.

Ya'll are giving out garbage and contradictory information and getting pissy when someone doesn't immediately accept it.

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Thank you so much. Thankfully, there are still some people who actually want to help and stay focused on the topic.
At least someone actually read the post.

Pastywhyte said:

OP should run some blank media and perhaps test the genetic samples to determine what the vector is. Until that is done little can be achieved via bickering.


I'd like to not have to lock this thread, would be nice to see some positive resolution at the end. But let's tone it down a bit so I don't need to put on my mod hat.

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WhiteDemon said:

Pastywhyte said:

OP should run some blank media and perhaps test the genetic samples to determine what the vector is. Until that is done little can be achieved via bickering.


I'd like to not have to lock this thread, would be nice to see some positive resolution at the end. But let's tone it down a bit so I don't need to put on my mod hat.

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Thank you so much. I’ve made some PF Tek jars and left them uninoculated to see if the sterilization is what’s failing this year.
Many of the users who replied to me had no real interest in reading the post or helping. Their intentions may have been good, but their inability to understand it made their responses useless. They assume that if the results are bad, it must be because I don’t know what I’m doing and that I’m a complete noob. They can’t seem to consider any other possibility. Correlation does not imply causation.

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Quote:

Pastywhyte said: But let's tone it down a bit so I don't need to put on my mod hat.

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I bet that hat looks good on you bro!