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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis?
    #29651492 -

I’ve been using Castellani method with 2ml vials but am a bit concerned that the amount of nutrition in my agar wedges dropped into the vial may be too high?

Am I overthinking it or should I be attempting to minimise the amount of nutrition that ends up in the vial?

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Re: Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis? [Re: bongoman] * 1
    #29651497 -

I've done 0,5% and 2% agar to 2ml vials with RO water. Both have revived within 4 days after 20 months in stasis.


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis? [Re: Kloakk] * 2
    #29651507 -

Thanks! I can always trust you kloakk for quick and accurate answers - appreciated!

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Re: Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis? [Re: bongoman] * 1
    #29651510 -

bongoman said:
Thanks! I can always trust you kloakk for quick and accurate answers - appreciated!


Thank you too man I'm happy to help, this question came just in time, it's only the last month I have tried reviving the cryo vials I did when I first started doing them, revived 4 so far and everyone came to life real quick.

I did a couple of extra vials from the same plate the first run so I'll try reviving the next in a couple of more years.

:havesomescience:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis? [Re: Kloakk]
    #29651830 -

Did your agar solidify the water? Mine have basically swelled up to where they look like sslc with a drop or 2 of water on top.


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Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
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Re: Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis? [Re: Bigworm] * 1
    #29651852 -

I try to slice horizontally close to the surface so I just get the top, but that's also so I can fit more slices in the tube since they're only 5ML and I pack em tight.

My understanding is you also want to minimize air, so I fill the water to the brim too.


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.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.
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🧪 Castellani Preservation | 🔬 The Slidewich | ☠️ Touchless skull sterilizer | 📸 Agar plate scanner


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Re: Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis? [Re: Bigworm] * 1
    #29651921 -

Bigworm said:
Did your agar solidify the water? Mine have basically swelled up to where they look like sslc with a drop or 2 of water on top.


Not really, I can see that the wedge is smaller coming out then going in so I guess some agar made it to the water. I have been dumping out the water leaving the wedge behind in the vial before I get it out and it's pours like water, if that makes sense :lol:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis? [Re: Kloakk] * 1
    #29652207 -

Yeah makes total sense. I think I may have over packed mine and the agar swelled. Oh well as long as they revive, or at least my dried grains revive I'll be happy


--------------------
Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum





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Re: Castellani method - do I need to minimise the amount of nutrient agar in the vial to promote stasis? [Re: Bigworm] * 1
    #29652217 -

Bigworm said:
Yeah makes total sense. I think I may have over packed mine and the agar swelled. Oh well as long as they revive, or at least my dried grains revive I'll be happy


So far 100% has revived, but not that old yet, and thanks for reminding me to try drying grains. I've made a couple of lids but still haven't actually dried a single grain, soon :muppet:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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