Here's two jars. First one was fully colonized when it was shaken and tested for recovery.

From left to right: 12 hours, 24 hours and 48 hours.


Second one was at around 85% colonized when it was shaken and tested for recovery.

Left to right: 12 hours, 24 hours and 48 hours.

Rasmodius said:

Question: do you expect the recovering spawn to eventually get all the way as dense as OP's pic / before it was shaken? I know it can be sent sooner, but is it ever worth waiting?

I do pint jars in twos for each shoebox. Had two pints going recently, both looked very densely colonized, but one had a couple straggler grains, so I gave it a BNS just to be safe. Didn't shake the one at 100%. Shaken jar bounced back okaaaaay, but after 4 or 5 days was still nowhere as densely white as it was before. Still fully colonized though, no open grain spots.

They were both headed to the same shoebox, so I spawned them to separate sides with a wall of plain coir separating them. They've colonized and pinned pretty much identically in terms of speed, coverage, etc, so if the shaken one was fighting something, it wasn't very serious.

So it seems like "technically recovered but not as strong as OPs pic" is fine, yea?

Shoebox w both sides after a few days.

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With enough time it should get just as dense, but the only time I let them go that long is if I don't have the space or tub to spawn them. If you look at the first jar you can see it's fully recovered, but quite faint for cubensis, I think this is because the grains was already colonized and are only recovering and not jumping onto uncolonized grains. I would have no worries about spawning the jar right now.

In the second picture you see more dense growth because the mycelium is jumping over to uncolonized grain, or that is my assumption anyway

LobsterClaw said:

I guess i will just keep them going and see if anything happens?

I want to do the deep dive into the agar thing but it was pretty complicated when i looked at it, id need to really sit down and do deep dive. Sounds worth it though, I guess eventually you can be completely self reliant by cloning and keeping your own culture indefinitely is that right?

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Well if the bags keeps inflating by themselves they're probably gone, yeast is a common contamination.

I know how you feel, agar felt overwhelming when I first looked into it as well. But as wowimflabbergasted says it isn't really that hard.

You will need some sterile petri dishes, preferably a media bottle, agar agar powder and some light dry malt extract.

And yes, with agar you can take clones and put agar in stasis to keep in cryovials or media bottles. I actually just revived my oldest cryovial, it has been sitting in a 5ml PP5 container in room temperature for 1,5 years.

wowimflabbergasted said:

Agar isn't really difficult. If you can make jello then you can make up agar plates.

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