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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Agar Plates and Removing Hidden Contaminants. A Visual Notebook [Re: DV8DUG]
    #29546505 -

Thank you for putting this together, as a newbie this makes the agar selection process way less intimidating.

Question — how does one put agar to BRF cakes? Just pop a gloomp in on top same as GS, or do you inject it, and if so, how?

(I’m still working on getting clean myc on agar, but BRF cakes have grown well for me when the same MSS put to grain went a couple shades of green, so I’m already a believer in its ability to get fruits out of nasty nasty syringes. I’d thought it was a matter of isolating the contams but I shredded one of those cakes into coir and saw nothing out of the ordinary and it smelled like pure essence of fresh clean shrooms; that box is about to pin with no signs of grossness, so you could feed me some line about the synergistic effect of BRF and vermiculite and I’d be inclined to buy it.)

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Re: Agar Plates and Removing Hidden Contaminants. A Visual Notebook [Re: Yermahm]
    #29546512 -

Thank you! Fantastic write up. As you know this took a lot of effort. It is very much appreciated.
:thumbup::mushroom2:
:goodday:


--------------------
"The cosmos is within us. We are made of star-stuff. We are a way for the universe to know itself."

Carl Sagan

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Re: Agar Plates and Removing Hidden Contaminants. A Visual Notebook [Re: Yermahm]
    #29546717 -

Yermahm said:
Thank you for putting this together, as a newbie this makes the agar selection process way less intimidating.

Question — how does one put agar to BRF cakes? Just pop a gloomp in on top same as GS, or do you inject it, and if so, how?

(I’m still working on getting clean myc on agar, but BRF cakes have grown well for me when the same MSS put to grain went a couple shades of green, so I’m already a believer in its ability to get fruits out of nasty nasty syringes. I’d thought it was a matter of isolating the contams but I shredded one of those cakes into coir and saw nothing out of the ordinary and it smelled like pure essence of fresh clean shrooms; that box is about to pin with no signs of grossness, so you could feed me some line about the synergistic effect of BRF and vermiculite and I’d be inclined to buy it.)


You might find BRF pucks interesting


--------------------
Feeling lost here? We get it. Here's the most up-to-date guide as of 2026.


I know I'm a fool, but that's why I keep trying to learn.
:peace::heart::mushroom2:

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Re: Agar Plates and Removing Hidden Contaminants. A Visual Notebook [Re: Yermahm] * 2
    #29547514 -

Yermahm said:
Thank you for putting this together, as a newbie this makes the agar selection process way less intimidating.

Question — how does one put agar to BRF cakes? Just pop a gloomp in on top same as GS, or do you inject it, and if so, how?

(I’m still working on getting clean myc on agar, but BRF cakes have grown well for me when the same MSS put to grain went a couple shades of green, so I’m already a believer in its ability to get fruits out of nasty nasty syringes. I’d thought it was a matter of isolating the contams but I shredded one of those cakes into coir and saw nothing out of the ordinary and it smelled like pure essence of fresh clean shrooms; that box is about to pin with no signs of grossness, so you could feed me some line about the synergistic effect of BRF and vermiculite and I’d be inclined to buy it.)


I used to use every plate I made and the ones that were contaminated that I would transfer from I would totally put to BRF just because of the medium and its synergistic behaviours.

I would eliminate the dry layer in my jars and just plop the whole agar plate onto the BRF. I use condiment cups so the fit is better.

I've got a lot of fruits from ugly agar by doing this.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Agar Plates and Removing Hidden Contaminants. A Visual Notebook [Re: DV8DUG]
    #29549520 -

There’s 2 plates of Melmac culture and the colouring seems to change slightly and I’m unsure it’s just the mycelium or something else.

One at 12 o’clock on the plate and the other a little here and there. I don’t want to drop them to grain if there off at all.

Any clarification would be amazing either way 😁.



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Re: Agar Plates and Removing Hidden Contaminants. A Visual Notebook [Re: DV8DUG] * 1
    #29613448 -

Ohhhh, I am very excited about this!  Fingers crossed that it’ll clean up my dirty APE and its nasty halo!





Thanks for sharing this tek! 

Edited by Chelonia (06/29/26 10:24 AM)

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Re: Agar Plates and Removing Hidden Contaminants. A Visual Notebook [Re: Chelonia]
    #29624856 -

Terrific guide especially for new growers, I had to re-read it twice to start to pick up on some of the patterns. It's very humbling to not be able to pick up on everything quickly, but now at least in the pictures I feel like I'm understanding the basics of the patterns better. It seems like ordered and consistent behavior is important. So identifying how growth reacts to shape of the transplanted material and checking if the same edges are causing the growth to appear similarly in other parts of the plate.

I naively thought that strong growth was the most important aspect, and it would be as simple as contaminated plates with visible goop/mold, but that's not the whole story. Order, uniformity, and agar texture are important. It's really about taking the whole picture and looking at everything from different angles, asking your agar questions about what you're seeing and mentally testing and confirming it. And of course, testing and transplanting irregular regions of growth is the best way to test and continue to learn it ourselves.

Identifying these patterns and observations take time and dissecting my own failures and successes. However, this guide is helping me start with wheels on my bike instead of pogo sticks. Thank you!

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Re: Agar Plates and Removing Hidden Contaminants. A Visual Notebook [Re: DV8DUG]
    #29677263 -

Thanks for the guide! Super helpful!

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Re: Agar Plates and Hidden Contaminants. A Visual Notebook [Re: phenyl]
    #29677268 -

phenyl said:
AspectOfTheCreator said:
Anyone know if the trench technique would work if the contaminant was a mold?


won't do shit


So trenching is basically useful only for bacteria?

DV, for which contaminants do you find it useful?

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Re: Agar Plates and Hidden Contaminants. A Visual Notebook [Re: koma23]
    #29683658 -

I use it to clean anything other than mushroom. I also store my plates vertically so any bacteria just runs down. Gravity can be our friend.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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