Kloakk said:

I keep my lids tightened  as soon as it cools down after sterilizing with both LI and LC  I open it ones to inoculate LC(josex poke) and once to inoculate LI(colonized agar).

In my opinion the josex's poke is superior to agar when talking about getting a clean transfer to media. With the poke you just slightly open the media bottle and squirt the poke and sterile water into the media bottle with sterilized broth, that little speck of mycelium will expand and grow until there is no nutrients left and that is why Lew said that LC basically turns into LI when there is no more nutrients in the broth.

The downside here is that if there is anything riding along with the poke or getting in the media bottle during inoculating there is nutrients in the broth for contaminates to grow and expand.

With LI you only get as much mycelium as you add colonized agar, might get a little nutrients from the agar as well, but there will not be nearly as much growth, this means that if you are going to use LI as a master you will need to add a lot of agar. The good thing about LI is that there isn't any nutrients in it.

Downside is that it takes a lot longer to inoculate LI and the lid on both the plate and the media bottle will be completely off while doing the inoculation, hence increasing unwanted guests in your LI.

They are both great TEK's, but in my opinion LI is for storage and keeping a master that has to go back to agar before grains, since you don't use it all in one go. LC is for expanding and colonizing grains or AIO's fast. Hope that clears things up at least a little

---

You’re fucking good bro.

LewDoja said:

How long ago were these swiped, and how long till first signs of myc?

First on the left has potential, the smaller one .... might be the bacteria causing stress.

Id get some samples in a cabin soon, maybe storing them vertically so the myc can climb while the bacteria falls, to get cleaner transfers.

Maybe it's the recipe.....Karo and potato flakes is pretty fkn lazy too

Second one, not quite convinced yet its cube myc there might be something riding along... beside the bacteria.





How did you swipe the plastes?

---

PuffM4gicDr4gon said:

I have no idea if this is right, but my stoner logic says that bacteria should be less of an issue with LI because if you're using distilled water the only food source is colonized agar. I know very little about biology, so I'm probably missing a whole bunch of essential information.

---

I agree with this stoner assessment
Also not knowing if this is true, but it makes sense. I have not done a lot of LI because I use a SAB and more then once have used LI and ended up inoculating jars with sterile water only

I went as far as trying to invent the wheel to get a more consistent LI pour.







Then I got some help, thank you Seldom Seen and I moved over to LC and SSLC for expanding and speed.

AZ Starman2112 said:

You’re fucking good bro.

---

Thanks AZ I often feel I'm just confusing everyone more when I try to explain something

AZ Starman2112 said:

Yeah, I bought the 2ml, 5ml & 20ml. I did them all at the same time in my PC in one shot with DW. I use the 2ml for storing 5-6 8mm punch outs of a culture when I spawn. I keep a spreadsheet on them and I make a note on how the culture performed after spawning. My intent is to take strong cultures and be able to revive them, hopefully cleanly, but knowing they are going to produce results. Of course this is all hypothetical and time will tell. Here are the links…

20ml

5ml

2ml

---

llenaballena said:

I've got a dried penis envy stipe that I would like to try cloning. It was dried at 30 degrees and has been sitting in a jar for around 4 months. I plan to moisten it in distilled water and scrape the insides onto agar plates.



Any advice on better ways?

---