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Re: Ask Quick Questions, Get Quick Answers [Re: andymc] * 2
    #29514553 -

You could re-melt it and stir in some more agar, probably


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How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 1
    #29514590 -

:whathesaid:

brominegeek93 said:
added too much water to my agar mix, so it didn't get a solid consistency. I tried using a hair drier to dissipate some water but it just turned into a mushy mess. Is there any chance these plates will turn into solid gel blocks or should I scrap it and start again?




And what food colour did you use? Nice sky smerf blue


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.
"How do you eat an elephant? One bite at a time."

Edited by Blizzloo (03/13/26 01:51 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Blizzloo]
    #29514677 -

Just used the cheapest REWE brand food coloring - if you don't live in germany you're out of luck, I'm afraid


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When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 2
    #29514731 -

After you cook the plates, you can slightly unscrew the lids and they will dry out over time.  My holy grails are modified for airflow, so over a few months they can dry out completely if I don’t store them in a ziplock.  If time is a concern, I’d just start fresh.


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O son of Kunti, I am the taste of water, the light of the sun and the moon, the syllable om in the Vedic mantras; I am the sound in ether and ability in man.

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Re: Ask Quick Questions, Get Quick Answers [Re: Caffeineo] * 5
    #29514770 -

Caffeineo said:
WTF is the dark color on the bottom of this plate? I have suspected it is some kind of mold or contam. This is T3 trying to get rid of it. The circled part is where I took a transfer to T4 trying to get it clean. There is also some trype of contam opposite where I took the transfer. TIA




To me what stands out is that super dark wedge in the center. It looks like they myc is carrying the pigment out of that wedge and distributing it exponentially. The pigment is clearly in the tissue and not an extracellular secretion.

That plate is surely contaminated, but I would venture that the pigment you are seeing is an artifact, not something the mycelium or a ride along is producing.

Another observation is how it is throwing a lot of that pigment at a contaminant on the right side there where that crescent moon shape is. (could just be another phenotype running too)

If it is from a contaminant, that culture is likely a complete loss. If it is a fungal pigment it is likely melanin. I use pigment like that from the bottom view as a way to differentiate between aspergillus, cladosporium, and penicillium. Penicillium typically doesn't make pigment like that and cladosporium is jet black all the way through. It's just a first step and not a 100% way to differentiate them. Just a quick filter in case the colonies haven't made spore or the spore is too thick to see through, or if I am lazy and don't want to use the scope.


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Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29514918 -

ThurmanMurman said:
LewDoja said:
Im team modified shoebox, and can always tape up the holes more if needed

Also the staggering of elevation of the holes create covection currents allowing passive evaporation whilst not over drying.

Its a balancing act, think about FAE... as Fresh Air & Evaporation (balance)


Lew in da house? Good to see ya, homie!

Anything specific to how you're modding your shoeboxes? I halfass kicked the idea around myself and was gonna wing it cuz like ya said - tape handles those happy little mistakes.


Thanks bud!

Pastey put in the hard work..
Several up high on small side, couple lower (above sub level)

Edited by LewDoja (03/13/26 06:55 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29514922 -

Splittinghairs said:
Elixirel said:
I have a SAB but no time or space for preparing agar, no pressure cooker, and no funds to buy one for a while.  I am resigned to spend a bit more on premade plates to do some agar to agar transfers and not waste time.

Could that "nutrient agar" be PDA? That wouldn't be more expensive  though , right?


Instapot works just as well.


So pftek?

Pda shouldn't make it more expensive,
But nutrient agar could be anything.... Malt extract,  nutritious yeast,  Potato/dextrose etc.

If they're not listed i'd look for another.

But honestly seems like a lot of work and expensive just to make some cakes with.

There's a thread by Rev? Pristine pf cake for cleaning up cultures.

The money saved could buy you an IP , or second hand PC.


--------------------
A_wise_man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"


🎶  KIND TC's - by: Tri  🎶

Edited by LewDoja (03/13/26 08:16 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29515007 -

i am in the process of building a LFH using gordo tek. I didnt realize till the end that the fan shouldnt be facing the filter directly. If i install a wood barrier or something to keep it from blowing on the filter directly would it still work?


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~Wherever you go in life, there you’ll be~




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Re: Ask Quick Questions, Get Quick Answers [Re: noomer] * 1
    #29515011 -

It's not a LFH.  It's more like a small ffu.

https://www.shroomery.org/forums/showflat.php/Number/29509225/page/1

Anyway, since you've already built it. I would just turn it on and do a lighter check to see how much turbulence there is.

But I would also read through the comments in that thread, so you have realistic expectations about your workspace.

And yes, probably putting a barrier would help if you do have a lot of turbulence. But again it's not LF.  Which means that your workspace isn't going to be very large either way.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 1
    #29515012 -

Weigh out your ingredients and write it down (with brand names,)
And then you'll have more control, and able to manipulate it to your will.

Its very surprising how a little fluctuations in agar % can have a dramatic effect.

Such as ~3%ag will be much harder for bacteria.

1.67% is about the lowest i go with most brands but not much over 2-2.2%

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 1
    #29515018 -

tree frog said:
It's not a LFH.  It's more like a small ffu.

https://www.shroomery.org/forums/showflat.php/Number/29509225/page/1

Anyway, since you've already built it. I would just turn it on and do a lighter check to see how much turbulence there is.

But I would also read through the comments in that thread, so you have realistic expectations about your workspace.

And yes, probably putting a barrier would help if you do have a lot of turbulence. But again it's not LF.  Which means that your workspace isn't going to be very large either way.


TY TREE


--------------------
~Wherever you go in life, there you’ll be~




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Re: Ask Quick Questions, Get Quick Answers [Re: Rukus]
    #29515020 -

Rukus said:


Is this a good wild bird seed for grain spawn?


As far as I know all wild bird seed should work. It's more about what grains or seeds they are instead of the nutrient breakdown. Some people pick out the sunflower seeds I think but ive read that isn't something you need to do. I've never used wbs though. Just read about it.


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The closer you get to the meaning the sooner you know that you're dreaming

How the Bushman gets stuff done

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29515078 -

I got a free swab and it was labeled Pink Buffalo. I read a little about it and was hoping I could end up with a clean plate. Much harder than I expected.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29515080 -

WOW. Thanks for the detailed reply. Just simple agar plates. I guess it is toast.

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Re: Ask Quick Questions, Get Quick Answers [Re: Caffeineo] * 3
    #29515144 -

I wish we could be done with the GordoTek "LFH" thread... Save everyone some time, material, money, and patience.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29515222 -

DV8DUG said:
I wish we could be done with the GordoTek "LFH" thread... Save everyone some time, material, money, and patience.



:foshizzle:


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29515265 -

Yeah, well said. For what it cost for one of those, save up an extra $50 and buy the Chinese one on ‘zon and let’s move forward. Or start a Gordo thread. Or just make a SAB! Just my opinion, but for fuck’s sake!

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29515359 -

I would have thought adding more agar would result in a higher contamination risk?
How much % potato flour/flakes would you add to the mix?


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 1
    #29515364 -



It's a 66qt tub and there's still some pins on the top. Im scared to flip it over but I might need to just nut up and do it.

:emocry:

Update: I found a sheet pan that fit nicely inside the tub, flipped upside down it had just enough clearance not to harm the top pins. I then flipped the whole tub over while holding the pan in place and had someone else carefully remove the tub.

All shroomz are now tucked away safely in the dehydrator :salute:

Edited by RhyzoPhreak (03/14/26 04:11 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: AstroSporeFiend] * 6
    #29515469 -

AstroSporeFiend said:
Yeah, well said. For what it cost for one of those, save up an extra $50 and buy the Chinese one on ‘zon and let’s move forward. Or start a Gordo thread. Or just make a SAB! Just my opinion, but for fuck’s sake!


The Official Why Your Favorite Mushroom Influencer's Tek Probably Sucks thread?


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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