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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper] * 2
    #29485918 -

I usually let them colonize the casing. They never thicken up and choke themselves like cyans in my experience.

Kept the lid on loose in the fruiting chamber until pins set.



These were the last trays I ran without a lid prior to pins.  The fruits were much thicker but the same sized tray only put our a couple of clusters.  And the substrate was much richer too.



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #29485931 -

PsiloPsychIn said:
Very excited to โ€œhearโ€ you will be sequencing the Komodo Islands Pan Cambodginiensis,
Tree Frog

As you may recall from a PM exchange, a therapist I supply told me the Komodo had a uniquely therapeutic effect on a person with chronic pain.

Having a good sense of the DNA sequence and where it sits relative to others would be a good first start. A full assay of the alkaloid profile would be even more helpful.

Thank you for all the research you do and the cultivation skills you share.



Well I won't be sequencing it. I will get it to someone who will and he's already down to run it so.

Kirking posted an alkaloid profile on them and it was like 4% psilocin.  Not much in the way of minor alkaloids. And how they are handled and dried has a lot to do with the psilocybin/psilocin ratio.

That's said, you might just have a soft spot for them and I think that matters frankly.

I trust and respect bisporus personally. But I haven't seen anything in alkaloids that suggests it's more than my fondness for them and the folks who taught me to grow them.

Not that we've quantified everything in them. But minor tryptamines likely aren't having much if any effect on our subjective experience.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29485950 -

https://www.shroomery.org/forums/showflat.php/Number/29244951#29244951

Kirkengs tests.

The neoxalapensis is the only one with enough minor alkaloids to make a difference imo.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29485960 -

tree frog said:
I usually let them colonize the casing. They never thicken up and choke themselves like cyans in my experience.

Kept the lid on loose in the fruiting chamber until pins set.



These were the last trays I ran without a lid prior to pins.  The fruits were much thicker but the same sized tray only put our a couple of clusters.  And the substrate was much richer too.





Choke by what means, shut off humidity and vent ๐Ÿ˜ณ?

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29485966 -

I mean cyans can do this.  And that mycelium is more prone to drying out and choking the substrate.



While bisporus can be left in your tent (or fruiting chamber) with very little misting needed if you just do this with your lid.  (i.e. flip it so that it's not losing moisture but still getting enough air that it doesn't get pin molds).



And they don't need to go into a tent really until they pin.  But I do since that's where the temperature is controlled.

In other words, I use different strategies for bisporus and cyans.  When my bisporus is ready I'll post in update in my AIO thread, or maybe just make a pan casing thread.

Anyway, these are cyans.  Cased and into the chamber with no lid.



Watered 24 hours later once.



Knots 24 more hours later.


And today they're pinning.

But bispo I would keep the casing from drying out at all if I can help it.  So I don't have to water.  And I do that with a lid on loose.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/15/26 08:39 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29485973 -

Foreighn2891 said:
tree frog said:
I usually let them colonize the casing. They never thicken up and choke themselves like cyans in my experience.

Kept the lid on loose in the fruiting chamber until pins set.



These were the last trays I ran without a lid prior to pins.  The fruits were much thicker but the same sized tray only put our a couple of clusters.  And the substrate was much richer too.





Choke by what means, shut off humidity and vent ๐Ÿ˜ณ?



and by looking at the colors on photos you got it seems that those are different genetics ๐Ÿง

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29485992 -

They're the same genetics.  Or at least, the same species.  Smelly had some that looked very similar to you earlier this winter.  Dark caps, small.

Anyway, these are all Coleville in different conditions.  Environment does a lot to how they express.











Edit:  Last night I was curious and searched my name and bisporus.  Over 10% of my posts are about bispo :lol:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/15/26 08:33 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29486008 -

tree frog said:
They're the same genetics.  Or at least, the same species.  Smelly had some that looked very similar to you earlier this winter.  Dark caps, small.

Anyway, these are all Coleville in different conditions.  Environment does a lot to how they express.











Edit:  Last night I was curious and searched my name and bisporus.  Over 10% of my posts are about bispo :lol:


man!๐Ÿฅฒ looks amazing, I can stuck watching them for an hours ๐Ÿคฃ  a specially the one in โ€œonionsโ€๐Ÿ˜ is accompanying plants making any difference ?

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29486018 -

Thank you so much for info you are sharing again ๐Ÿ‘๐Ÿป

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper] * 1
    #29486056 -

It's grass not onions :lol:

Wheatgrass so pretty thick blades. That tray fought cobweb mold too the whole time, so I went overboard on the grass. But it was my first experiment.

And yeah I like growing them with grass, it's certainly makes managing the casing easier.  Also speeds up pin time quite a bit.  But it also makes harvesting more finicky.

These aren't even cased, just top dressed with coir and compested manure, and sprinkled with rye grass seed.



And here you can see the white one got a little bit more light and thus more evaporation.  All the same tray.



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29486059 -

Foreighn2891 said:
Thank you so much for info you are sharing again ๐Ÿ‘๐Ÿป



Hey thank you for asking me about my favorite thing to talk about :lol:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29486060 -

I have a question for tree frog.  What is your opinion on using microgreen seeds in lieu of the grass seed for a tray rescue/grow?  Some kind of small seed like radish, broccoli, or a salad mix.  Then, in theory, you could potentially harvest mushrooms and edible microgreens.  Or am I (quite possibly) misunderstanding the actual mechanism of why your system actually works?

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Re: Official Copelandia/Panaeolus Thread [Re: Pit pat]
    #29486063 -

You know I haven't tried it yet. But I bought some Asian greens that I think would work well in a similar environment.

I think they might be a brassica.

Mizuna.

https://en.wikipedia.org/wiki/Mizuna

But you want something that's going to sprout and grow quickly, and also is going to tolerate high humidity and warm temperatures.

Edit:  And I do believe it's more than just environment, that seeds have endospores on them for different types of microbes that can promote pinning.  But brassicas (IIRC) seem to have some relationships with fungi.  Or more likely the microbes that live with them.

Edit two:  Dual harvest is also why I started with wheatgrass seed. We have a juicer.  But if I'm not in the mood for juicing, rygrass seed is a bit easier to manage.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/15/26 10:16 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29486067 -

Some of the varieties, like some I mentioned, can actually be ready to harvest in as little as 8 days.  The temperature shouldnโ€™t be a problem, but not certain about the humidity.  Seems like a nice experiment.

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Re: Official Copelandia/Panaeolus Thread [Re: Pit pat]
    #29486070 -

Yeah it's definitely worth a shot and I would love to see your results!

Also thanks for reminding me, I forgot I even had these seeds and have some projects I could try them on soon :smile:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29486079 -

:thumbup:
Looking forward to see how you incorporate them.

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29486087 -

tree frog said:
It's grass not onions :lol:

Wheatgrass so pretty thick blades. That tray fought cobweb mold too the whole time, so I went overboard on the grass. But it was my first experiment.

And yeah I like growing them with grass, it's certainly makes managing the casing easier.  Also speeds up pin time quite a bit.  But it also makes harvesting more finicky.

These aren't even cased, just top dressed with coir and compested manure, and sprinkled with rye grass seed.



And here you can see the white one got a little bit more light and thus more evaporation.  All the same tray.




are they differs somehow, those lawn seeds, are some of them quicker to grow?

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29486134 -

tree frog said:
My first grow took four weeks to pin :lol:  Also middle of winter.

Now I average more like two weeks.  But I quit counting probably a year ago.

Good management of the casing helps a lot though.  I'm sure over watering, and then drying out was part of why my first run took so long.



it's been forever since i tried bisporus. My jars (both rye and oats) are not showing any progress on bisporus (1 oats jar 1/28 and 3-4 rye jars from 2/6). I did a search and apparently oats should be more appropriate for coprophilic species due to thinner hull and more air gaps.

How long untill you typically see progress in a jars, on average, for oats vs rye? On your AIO stuff, maybe I replace like....25% of the oats with manure, or CV+? And i mean that purely for grain runs with intent to throw in bulk eventually.


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29486198 -

Thanks again for the detailed explanation Tree!

Foreighn2891 said:
Churpee Choozle said:
Wait wait. So trop is a cyan, that means PHV is a cyan cross too?



visually PHV looks identically like syans



Mine had pretty, bell-shaped caps, more so than cyans. I really enjoyed the trip. But yeah very cyanish looks




--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle] * 1
    #29486231 -

So I should be casing my Panaeolus grows with a peat based casing? I have Panaeolus Cambodginiensis and Panaeolus Cyanescens both growing in a 75% coir 25% poo substrate.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
๐Ÿ…ƒ๐Ÿ„ด๐Ÿ„ฐ๐Ÿ„ผ โ€‹ ๐Ÿ„ฒ๐Ÿ„พ๐Ÿ„ฝ๐Ÿ„ณ๐Ÿ„ธ๐Ÿ„ผ๐Ÿ„ด๐Ÿ„ฝ๐Ÿ…ƒ โ€‹ ๐Ÿ„ฒ๐Ÿ…„๐Ÿ„ฟ

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