conz0r said:

I desperately need to hop onto basic electronics work as end of the world training. You couldn't point to something for an avid reader/visual learner? Like novice stuff, I'm into my physics but engineering just never crept into my life.

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conz0r said:

I've got a cheap tomlov 1200x digital microscope too but not much license to use it at the moment.

All the transfers are looking happy btw, the Indian graveyard is coming along. I'm just modding some boxes at the moment

Doing test holes and nailing down my technique before doing the proper ones, I've done enough line work to know the gist. I'm making 4 all in one tubs inspired by tree-frog. 1.8 L capacity for 1L of material, 5 filters on top, one on each side. Then 4 1L spawn tubs, 5 filters on top in dice pattern.

I'll get back when ones done.

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The easiest (and least fume intensive) way to get properly sized holes is to use a step bit.


They are like $6 bucks and VERY useful on plastic (and ofc wood if you do woodstuff). It made super easy work of my reusable plastic jar lids, adding clean, nonspurred, 1/4in holes on like 25 lids in like, 8 seconds per lid. (on metal....it might work but that's much more difficult)

Churpee Choozle said:

Wait wait. So trop is a cyan, that means PHV is a cyan cross too?

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visually PHV looks identically like syans

tree frog said:

I have scoped PHV and it looked like a cyan.  But it's possible the microscopic metuloid feature is recessive and was bred out.

And I don't know if Workman's RDU is the same as the red spored variety that Alan ID'd as tropicalis, and I couldn't find Alan's microscopy posted anywhere.  But this is Workman's gallery.

https://sporeworks.com/gallery.php?g=subgenus-copelandia&id=1

And stonesun's grows of the India specimen.

https://www.shroomery.org/forums/showflat.php/Number/20492704

https://www.shroomery.org/forums/showflat.php/Number/21919650#21919650

This one has photos of RDU also.

https://www.shroomery.org/forums/showflat.php/Number/22042059#22042059

But RDU maybe different than "Panaeolus cyanescens Australia Redspore", and it's the latter that Alan IDd as tropicalis

Maybe one of the older pan heads will chime in who would no better than I.  All of this was before my time tbh.

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tree frog said:

I just edited my last post too   The debate has been going on for awhile.

Anyway, when the India one is available again I'll look at it.  And I imagine it will get sequenced as well as the holotype.  And then we'll have a clearer picture.

Did you see my Advanced Mycology thread on Pans?

https://www.shroomery.org/forums/showflat.php/Number/29481222

Everything uploaded so far is sequenced and are distinct species.  (Bisporus, IND01, and SA02).  I'm waiting to here back on my chlorocystis.  I think there was a mix up because it definitely does not act like cyan, so it may have been a different sample I sent in that was ran (not trop or oregon).

Komodo Island (Cambo) is on agar and will get sequenced, as we have the original find well documented.  I have a bunch of different cyans but would prefer to use a sequenced specimen for the thread.

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tree frog said:

Yeah there's two cyans going around as bisporus.  The Marcos Island Florida specimen and the one from Monte Grande Mexico.

The Florida specimen was sequenced and it came back as a cyan.  The Monte grande specimen myself and another cultivator who is very familiar with the West coast bisporus observed and concluded was a cyan.

Based on mycelium, fruiting speed, etc.  I didn't scope it because I didn't have a scope yet, but it's definitely not the same species as the Coleville, San Diego, and the Ohio specimens.  And behaved like any other cyan.

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this one is certainly not a syan, at least visually appearance doesn’t show any sign of, but it seems that turning upward edges once it’s matured is just a nature of this specimen

tree frog said:

That's bisporus.[/quote

tree frog said:

That's bisporus.

They're slower to pin, mycelium doesn't blob, white edge around the edge of the cap.

A small number of my photos are here.

https://www.shroomery.org/forums/showflat.php/Number/29481231#29481231

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look, I had the same info from buddy who shared them with me, and he told the same, that they are slower, but early fall, when temperatures not yet dropped to -40 as it happens always 🤣 room temperature was around 24-25 and they grown to maximum size within 29 days from casing, but☝🏻 and it took another 10 days more before spores was developed

tree frog said:

My first grow took four weeks to pin

Now I average more like two weeks.  But I quit counting probably a year ago.

Good management of the casing helps a lot though.  I'm sure over watering, and then drying out was part of why my first run took so long.

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