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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Stipe-n Cap]
    #29405508 -

Hmm, thanks for this.

I found a 17 year old spore syringe that I am playing around with. Now I have a couple of more experiments.

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: memeticist]
    #29405553 -

Spores in water not as likely to be viable after that many years. Odds are they already germinated and died.


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:dragon:

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Dragonaut]
    #29405569 -

Yes, they are most likely dead. But maybe...

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: memeticist] * 7
    #29406002 -

Procrastination on throwing out old/dead spores will hopefully turn into "my plan all along"!



--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: YoshiTrainer] * 2
    #29406024 -

That's the way I feel :lol:

2000 looks like a good vintage to try

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: DERRAYLD]
    #29406163 -

:pipesmoke2:

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Stipe-n Cap]
    #29406597 -

:takingnotes:

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: fungusul]
    #29432874 -

:nice:

I was wondering about the amount of Polysorbate 20 used, let's say for 100ml of water :strokebeard:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Kloakk] * 3
    #29433037 -

Kloakk said:
:nice:

I was wondering about the amount of Polysorbate 20 used, let's say for 100ml of water :strokebeard:


I used one drop in 150ml distilled water. I think that may have been more than necessary; however, the spores eventually germinated in solution. (The spores in triton didn’t germinate)



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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: NotImlay]
    #29433097 -

NotImlay said:
Kloakk said:
:nice:

I was wondering about the amount of Polysorbate 20 used, let's say for 100ml of water :strokebeard:


I used one drop in 150ml distilled water. I think that may have been more than necessary; however, the spores eventually germinated in solution. (The spores in triton didn’t germinate)





Now this is interesting.  About how long did it take before you noticed growth?


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: NotImlay] * 1
    #29433100 -

NotImlay said:
I used one drop in 150ml distilled water. I think that may have been more than necessary; however, the spores eventually germinated in solution. (The spores in triton didn’t germinate)




Thanks NotImlay, then I have something to go on, appreciate it :hatsoff:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: kingboomer] * 2
    #29433113 -

kingboomer said:
NotImlay said:
Kloakk said:
:nice:

I was wondering about the amount of Polysorbate 20 used, let's say for 100ml of water :strokebeard:


I used one drop in 150ml distilled water. I think that may have been more than necessary; however, the spores eventually germinated in solution. (The spores in triton didn’t germinate)





Now this is interesting.  About how long did it take before you noticed growth?


I wasn't watching closely to when germination started. But from my images Nov 7 was the day I prepared the polysorbate and spore solution. The image of the colonized solution was taken on December 1.


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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: NotImlay]
    #29433115 -

Ok thanks!  I definitely have a few ideas I want to try out.


-Kingboomer


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: kingboomer] * 1
    #29433133 -

@Notlmlay nice job, what type of spores and how old were they?


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: YoshiTrainer] * 1
    #29433144 -

YoshiTrainer said:
@Notlmlay nice job, what type of spores and how old were they?


They are 4 year old cube spores. Jmf. I live in the desert. Spores tend to desiccate quickly here.


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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: NotImlay] * 1
    #29433182 -

OK I'll do it..


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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: the_chosen_one]
    #29433267 -

Following with interest


--------------------
How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: andymc] * 3
    #29448977 -

I've made 2 pint jars with 200ml distilled water, 2 drops of polysorbate 20, and 12mg of ATP.

Now comes picking out which old spores to try to re-hydrate.



All the spores in the pictured envelope are from 2007 to 2011. I've tried to revive many of these on agar with no luck. 0% success rate with this collection so far. I also have a GT cube print from Roadkill labeled as printed in 2005.

Going to try to revive my stuntzii print from 2008.

Any requests for the 2nd jar?

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: BlimeyGrimey]
    #29448998 -

Quote:
Any requests for the 2nd jar?


Serbica because it would be cool for old spores to make it into the grow along.

HVC because it might be Semperviva / Subtropicalis and there’s a certain poetry to reviving old semperviva spores.

Edited by po10xi8 (01/07/26 02:22 PM)

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: po10xi8] * 1
    #29449194 -

I'm going to try this too.  I've got prints from 2010-2012 that I'm working on.  So far one (out of 8) seems to have germinated after being rehydrated with (sterilized) tap water.

Unfortunately I wasted all my old exotic prints making 30-odd contaminated syringes in a previous attempt, so all I've got left are a variety of cubes I printed/swabbed myself.

Polysorbate should arrive in a day or two


--------------------
How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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