|
Shroomery - Magic Mushrooms Demystified
|
Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!
|
 jcripestl
Stranger
Registered: 11/06/01
Posts: 2
Last seen: 16 years, 7 months
|
 Help-contam spores hydrated from print 
#448888 - 11/06/01 12:52 PM (16 years, 7 months ago) |
|
|
Any chance that I can save my spores? I recvd prints from Hawk w/o instructions. No reply when I requested instructions. I assumed(!) that the contents were sterile and I added autoclaved water directly to the ziplock to hydrate and collect the spores. Upon plating- psuedomonas! Any chance that I can 1) plate on antibiotic plates or 2) make serial dilutions and streak for pure cultures?
Many Thanks
|
felixhigh
KIA
 
Registered: 06/24/01
Posts: 7,543
Loc: Ly
Last seen: 5 years, 12 days
|
Re: Help-contam spores hydrated from print [Re: jcripestl]
#448900 - 11/06/01 01:08 PM (16 years, 7 months ago) |
|
|
you seem to know about agar culture procedures but you need instructions for a print. now thats weird man. get another (or maybe several) and try again. hints: on the web - MMGG; on your hands - the mushroom cultivator.
good luck.
|
 NDK
member
Registered: 07/13/01
Posts: 186
|
Re: Help-contam spores hydrated from print [Re: felixhigh]
#449862 - 11/07/01 04:14 AM (16 years, 7 months ago) |
|
|
Just stick some on an agar plate and subsequetly isolate the myc from any contams that form. Repeat as necessary!
|
jcripestl
Stranger
Registered: 11/06/01
Posts: 2
Last seen: 16 years, 7 months
|
Re: Help-contam spores hydrated from print [Re: NDK]
#450490 - 11/07/01 06:23 PM (16 years, 7 months ago) |
|
|
NDK was right. As it turns out, I had dropped small drops on one plate and added ~ 1/2 mL to another. Although the bacteria grew faster, the myc is now showing up in areas that are free of bacteria and should be isolatable.
Thanks again.
|
Anonymous
|
Re: Help-contam spores hydrated from print [Re: jcripestl]
#450570 - 11/07/01 07:25 PM (16 years, 7 months ago) |
|
|
since when did spores come with instructions? I thought they were sold for identification reasons only.
| |
|
|
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
824 topic views. 28 members, 100 guests and 34 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
| | |
|
|
|
|
|
Threaded
Previous
Index
Next
Edit 
Reply 
Anonymous
