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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Offlinejcripestl
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Registered: 11/06/01
Posts: 2
Last seen: 23 years, 8 months
Help-contam spores hydrated from print
    #448888 - 11/06/01 10:52 AM (23 years, 8 months ago)

Any chance that I can save my spores? I recvd prints from Hawk w/o instructions. No reply when I requested instructions. I assumed(!) that the contents were sterile and I added autoclaved water directly to the ziplock to hydrate and collect the spores. Upon plating- psuedomonas! Any chance that I can 1) plate on antibiotic plates or 2) make serial dilutions and streak for pure cultures?

Many Thanks

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Offlinefelixhigh
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Registered: 06/24/01
Posts: 7,623
Loc: Ly
Last seen: 5 months, 9 days
Re: Help-contam spores hydrated from print [Re: jcripestl]
    #448900 - 11/06/01 11:08 AM (23 years, 8 months ago)

you seem to know about agar culture procedures but you need instructions for a print. now thats weird man. get another (or maybe several) and try again. hints: on the web - MMGG; on your hands - the mushroom cultivator.
good luck.

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InvisibleNDK
member
Registered: 07/13/01
Posts: 186
Re: Help-contam spores hydrated from print [Re: felixhigh]
    #449862 - 11/07/01 02:14 AM (23 years, 8 months ago)

Just stick some on an agar plate and subsequetly isolate the myc from any contams that form. Repeat as necessary!

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Offlinejcripestl
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Registered: 11/06/01
Posts: 2
Last seen: 23 years, 8 months
Re: Help-contam spores hydrated from print [Re: NDK]
    #450490 - 11/07/01 04:23 PM (23 years, 8 months ago)

NDK was right. As it turns out, I had dropped small drops on one plate and added ~ 1/2 mL to another. Although the bacteria grew faster, the myc is now showing up in areas that are free of bacteria and should be isolatable.
Thanks again.

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Anonymous

Re: Help-contam spores hydrated from print [Re: jcripestl]
    #450570 - 11/07/01 05:25 PM (23 years, 8 months ago)

since when did spores come with instructions? I thought they were sold for identification reasons only.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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