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Offlineboygenius
strangler

Registered: 06/15/02
Posts: 54
Loc: Middle of no-nuthin'
Last seen: 6 years, 1 month
Is burnt lid seal toxic to spores?
    #764310 - 07/21/02 12:05 PM (14 years, 4 months ago)

Last night I sterilized an empty wide mouth 1/2 pint jar to hydrate some spores from a print. Put it in the oven at 500 degrees for @ 20 min. I had a new lid on (upside down) with the ring loose. The gray ring around the lid blackened and smoked (smelled horrid) during the process. It left the jar with a slight brown tinge on the inside surface. My question is: Will this residue have any effect on the spores' germination? My intuition tells me there shouldn't be a prob. Anybody got any life experience re: this situation? Also, anyone know exactly what the little gray seal is made of?

Thanks much,
Elroy, boy genius


--------------------
"It's too nice of a day to be stupid indoors"  ~ Ren


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OfflineOrbita6974
journeyman
Registered: 07/05/02
Posts: 71
Last seen: 14 years, 4 months
Re: Is burnt lid seal toxic to spores? [Re: boygenius]
    #764365 - 07/21/02 12:23 PM (14 years, 4 months ago)

sounds to me like you shouldnt put a jar in the ove....maby just use distiled water.


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Offlineboygenius
strangler

Registered: 06/15/02
Posts: 54
Loc: Middle of no-nuthin'
Last seen: 6 years, 1 month
Re: Is burnt lid seal toxic to spores? [Re: Orbita6974]
    #764414 - 07/21/02 12:41 PM (14 years, 4 months ago)

Usually I PC my glassware before use. Last night it was getting late and the PC was full of a big ole birdseed spawn bag chattering away. Wanted to sterilize a container quickly, the oven was unoccupied, the jar went in. Just like Hansel & Gretel. I do use distilled water to rehydrate spores, but if your glass isn't sterile, you may as well use pond water.


--------------------
"It's too nice of a day to be stupid indoors"  ~ Ren


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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