ansr said:

Interesting. Doesn't seem to make much difference in your case. What did they look like before casing?

None of mine have a single pin.

Gonna make more spawn and try again

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Looked like this.



And I know it doesn't help much, but for the record, my trays ain't doing shit either and I have no idea why. I have a lot of zapo's colonizing trays right now so if I don't see any pins in my pan trays  within a couple of days I'll throw mine out as well, they are taking up space in my dreamers without putting out

tultedspoon said:

If you look closely pretty sure you can see where the hyphae are joining in some areas. I'm wondering if that means some of this was monokaryotic or if compatible dikaryotic myceliums were joining (anastomosis) forming new genetic material? Is there any way to visibly tell by the thickness or by how the hyphae arches towards each other? or is microscopy the only reliable way?

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When the colony switches from vegetative to reproductive growth, the hyphae will grow into each other instead of out, forming knots and pins.

The odds of a mono surviving the plate, your grains, and your substrate without mating or being taken over by dikaryons is probably close to zero.

And there's no way to really tell without a microscope.  Even on a plate a mono is hard to tell from a dikaryon in pans.  I'm not going to label which is which because I don't want anybody trying to crib when you really can't tell.  As I've had dikaryone look like either.




When they mated they did this ring along the leading growth of both colonies.  And I still used the microscope to verify the cross, and to go back and verify that my mono was a mono.

Shewey said:

Gordotek

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Mr Piggy said:

Shewey said:

Gordotek

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Mr Piggy said:

Shewey said:

Gordotek

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Stipe-n Cap said:

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Be kind you guys, gordo might take credit for shit he didn't invent, but he's not the devil right? That's Stipe's title

PuffM4gicDr4gon said:

Question:

I screwed up my casing and made it too alkaline. It's around 9 after pasteurization.

Am I still able to use this? If not, can I just add some untreated peat and repasteurize? Or am I better off starting over?

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9 is fine, I'll bet you have to mist that casing, what is the pH of that mist

PuffM4gicDr4gon said:

Good point lol ty Kloakk

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My pleasure
to release some pressure

PuffM4gicDr4gon said:

Question:

I screwed up my casing and made it too alkaline. It's around 9 after pasteurization.

Am I still able to use this? If not, can I just add some untreated peat and repasteurize? Or am I better off starting over?

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Wild timing. I just overshot, or thought I did. Was 10+ before pasteurization. So I added more peat and dropped it down to 8.

But wait, there's more...
After pasteurizing and cooling it had dropped to 5 to 6. So I just added some limey water and have it back in the IP. I should have stuck to the recipe and left it alone, but I got a new toy and wanted to play with it.

ReverendMyc said:

Wild timing. I just overshot, or thought I did. Was 10+ before pasteurization. So I added more peat and dropped it down to 8.

But wait, there's more...
After pasteurizing and cooling it had dropped to 5 to 6. So I just added some limey water and have it back in the IP. I should have stuck to the recipe and left it alone, but I got a new toy and wanted to play with it.

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lamech said:

Hey folks, been working on my first pans grow with monotubs for a minute and I've got a few Qs.
I'm seeing a lot of bruising (or is it aborting?) in one of my tubs. Would this be from misting, conditions or something else? And once they get quite blue is it worth drying and eating them? I've got about 100g of mostly very blue fruits I just harvested:


They came from this tub (photo from monday):


And I've got this other tub that I've been giving less FAE and which was not showing the bruising issue until today or yesterday, and which is also growing some pretty anemic looking fruits:

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As TF said growing em in monos can be tricky, good passive airflow in your room outside the tub is helpful in maintaining the right fresh air balance. I would reckon you’ve not enough air flow in those totes personally. Your fruits resemble mine when I’m waiting to dump the tub and have set it aside with the lid closed.

As for direct misting causing aborts it can and will happen with certain cultures, I’ve found some cultures tolerate misting better than others, general advice is to always select many cultures from a germ plate and run them all searching for the most tub tolerant line. Not all cuts will tolerate a tub, that being said I will definitively say with enough searching any species and locale, wild or domesticated has the potential to be fruited in a mono, selection is key to find something tolerable to your personal environment.

Further growing in tubs for pans is hardly a one size fits all as outside factors of generalized rh, temps, air flow will affect the overall approach to dialing in your tub. Eg I find growing them in tubs takes an active approach and generalized curation of intentionality in developing a feel for them so you can make your adjustments on the fly.

You’re basically there though, I’d adjust your air exchange and compensate with misting to uphold moisture/evaporation. As well don’t be afraid to shelve the current culture if they remain uncooperative, your time may be better spent searching for one more cooperative to your capabilities.

As for misting as well, cyans can be less forgiving and a general rule I have for misting with them is during growth I mist enough to wet the caps however towing the line where by the caps have essentially evaporated the mist after about 5-10mins. But again this is curated to my personal environment, when growing in a different space with say higher temps and lower general eg the amount of misting to achieve this is quite a bit more than cooler and higher general rh…

The bruising could be drawn from underlaying health issues of your sub as well, keep an eye out for mold or other contamination that could present itself in the coming days, if you do see mold spring in the coming days I’d lean more towards that being the cause of the aborts than any issues with misting and conditions, especially considering the late stage aborting.

But hey man like I said you’re basically there, keep trying and developing your feel for them, they’ll come around eventually it’ll just take some time, in the meantime you got some to sample. Hell eating em may even make em like you more

Just suggestions, far from absolute rules, you got this!