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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29351472 -

tultedspoon said:
I've already gotten some growth in cups from a spore solution, is this wrong? or not ideal? I'm attempting custom dirty tek, with straight to soil. I've had successful mycelium growth with just spores, spores in water, and straight mycelium transfer if it's fresh (which sometimes is hit and miss), and sometimes mycelium in solution.

I use a miniature straw with my finger to drop the solution in several spots over miniature cups filled with moisture control soil. I did try "pasteurizing" soil with boiling water for some time, but oddly no growth, so there must be some type of symbiotic relationship with whatever is naturally in the soil. It seems like just getting the soil wet and then transferring some solution works the best. I'm still optimizing though. Some of the cups looked really good, but I got greedy and took chunks out and made new cups, and I probably would have had fruits by now if I hadn't cut all the cups in half. None of the cups fully recovered like I thought they would. I potentially exposed the mycelium to contamination, most of the cups still smell fine though.

I know people say overlay isn't usually a thing but I'm almost certain one of my cups has it due to incorrect conditions.




I can't say I've heard of "dirty tek", but the preferred method when starting from spores is to put it on agar. Take transfers to a new agar plate and if it looks clean put that to sterilized grains. Let the grains colonize 100% and then send those grains to pasteurized substrate. Let it colonize the substrate and then case it with pasteurized casing.

Boiling water is not pasteurization, you should get your substrate to field capacity and then heat it until the middle of the substrate is around 60-70°C(140-160°F) and then let that sit for about 2 hours. It is going to take some time because however you are heating it, no part of the substrate should be over 70°C or it can be partially sterilized.

Overlay can happen, some species more then others, but that is pretty normal.

You could go from agar to AIO jars or bags, but that would still need to be sterilized and all work(before going to tub or tray) should be done with a SAB or flow hood.

If you have a clean LC you could use that to inoculate sterilized grains or AIO's. It sounds like you are just doing spores to untreated soil and yeah, that is going to be a problem, I'm sure the mycelium will try to find a way, but I would think the chances of success is very low and you are wasting both spores, money and time..

Do you have a pressure canner or autoclave? And do you have any pictures of how and what you are doing any of this?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk]
    #29351508 -

My bispo is taking a while to pin. All healthy growth, was a bit to wet there. It's mid 10s here atm. I'm guessing it's temp related for sure. Is is just a waiting game now? Or do I have to get that temp up somehow?


--------------------
I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

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Re: Official Copelandia/Panaeolus Thread [Re: el boso]
    #29351533 -

el boso said:
My bispo is taking a while to pin. All healthy growth, was a bit to wet there. It's mid 10s here atm. I'm guessing it's temp related for sure. Is it just a waiting game now? Or do I have to get that temp up somehow?



Mid 10s? I'm assuming celsius, then yes you got to get it warmer boss.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon] * 1
    #29351534 -

Post some pictures of your set up and include a picture of the miniature straw you’re using. Is it a capillary tube?

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29351653 -

Just to clarify, when I said it won't overlay. I was referring to one species, bisporus.

Other species, yeah you may need to manage the conditions.  Depending on the culture.Overlay is when that puffy mycelium on top of a casing dies.  So exposing a cyan to a lot of evaporation when it has a lot of puffy myc on the surface, the top layer will die and then sometimes it will fruit up through the dead mycelium and sometimes it won't.

For folks like baba who grows in monotubs, it's really a non-issue because the humidity is very high during initial knot and pin formation.

If you aren't growing in a monotub, but a tent, you'll have to figure out a strategy to manage it.  High lipped trays, loose lids on trays, fruiting right after casing.  All of those are strategies to keep the relative humidity high close to the surface during initial pin formation (first flush).

Edit:  And I missed the OG's strategy (mother nature), which is growing
them in grass.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 1
    #29351864 -

Kloakk said:
I can't say I've heard of "dirty tek", but the preferred method when starting from spores is to put it on agar. Take transfers to a new agar plate and if it looks clean put that to sterilized grains. Let the grains colonize 100% and then send those grains to pasteurized substrate. Let it colonize the substrate and then case it with pasteurized casing.

Boiling water is not pasteurization, you should get your substrate to field capacity and then heat it until the middle of the substrate is around 60-70°C(140-160°F) and then let that sit for about 2 hours. It is going to take some time because however you are heating it, no part of the substrate should be over 70°C or it can be partially sterilized.

Overlay can happen, some species more then others, but that is pretty normal.

You could go from agar to AIO jars or bags, but that would still need to be sterilized and all work(before going to tub or tray) should be done with a SAB or flow hood.

If you have a clean LC you could use that to inoculate sterilized grains or AIO's. It sounds like you are just doing spores to untreated soil and yeah, that is going to be a problem, I'm sure the mycelium will try to find a way, but I would think the chances of success is very low and you are wasting both spores, money and time..

Do you have a pressure canner or autoclave? And do you have any pictures of how and what you are doing any of this?



No pressure cooker or autoclave. Trying to keep this simple and natural. I know it's possible I just gotta get ratios right. The dirty tek isn't a thing, but we could make it one. I encourage anyone to try it and figure out best ways to get fruits. Now I'm not hoping for great flushes, but that's ok. If I can just get several cups fruiting here and there as I slowly collect for a holiday without a ton of supplies, it's a win in my book.

I may post pics at some point, I don't want to compromise my connection and not sure if uploading a picture would do that. Maybe a hosting platform of some sort i'll try to look into it.

I'm avoiding grains cause I know the contamination when it happens can make some nasty bacteria. I'm avoiding honey because of ants, but I may try a dilute honey agar if it comes down to it. And ok yea boiling water is 212F so I prob got that soil too hot and "sterilized" some of it. The soil is already bought, the spores are collected naturaly, so only thing I'm wasting is time.

Some of these setups I have are an absolute joke, but some of the worst ones actually have really really tiny pins believe it or not. I have a really tiny jar no bigger than my thumb with just scraps of cardboard lasagna-style I inoculated with mycelium and some spore solution. It's been sitting for 2 months in the dark. The growth is pathetic, but believe it or not there is a super tiny pin popping up from a primadora. Literally the tiniest pin I've ever seen. IDK if this could ever fruit or not, I can't imagine it growing big, but maybe there is some way to use this miniature pin to an agar at some point idk. Maybe I should try a random jar with just some cardboard at some point for a slow maintenance-free method. I've like never even had to water it, it's just damp.

And to the question about the capillary tube, lmao, no it's a miniature milk carton straw. If I can get some pics up I think I should because some of these setups are so bad but good u wouldn't believe it unless you saw it. Once I get more experience I'll set up mini trays and mini monos. The dirty tek takes some trial and error first. I don't want to waste a ton of soil.

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon] * 4
    #29351875 -

tultedspoon said:
The dirty tek isn't a thing, but we could make it one.



:justno:

Or you could listen to the people who know that they are doing and actually get some fruits.

Winging it doesn't have a great track record with pans.  If you just want to be lazy then try this with some cubes, you are far more likely to get a few fruits.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy]
    #29351944 -

when i crack the code I'll make sure to make an official tek that can be replicated by those who don't want to use grains and are looking for a more natural grow cycle. Even with medical marijuana strains, there are some non-hydro grows in soil that can be stronger and more psychedelic than hydro grows. I have my theories, you can have yours.

There is still much to learn, even from reading for the past months I am constantly seeing evolving grows how things are done. That alone is proof that we should all be asking more questions instead of taking everything as absolute truth.

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29351950 -

tultedspoon said:
No pressure cooker or autoclave. Trying to keep this simple and natural. I know it's possible I just gotta get ratios right. The dirty tek isn't a thing, but we could make it one. I encourage anyone to try it and figure out best ways to get fruits. Now I'm not hoping for great flushes, but that's ok. If I can just get several cups fruiting here and there as I slowly collect for a holiday without a ton of supplies, it's a win in my book.

I may post pics at some point, I don't want to compromise my connection and not sure if uploading a picture would do that. Maybe a hosting platform of some sort i'll try to look into it.

I'm avoiding grains cause I know the contamination when it happens can make some nasty bacteria. I'm avoiding honey because of ants, but I may try a dilute honey agar if it comes down to it. And ok yea boiling water is 212F so I prob got that soil too hot and "sterilized" some of it. The soil is already bought, the spores are collected naturaly, so only thing I'm wasting is time.

Some of these setups I have are an absolute joke, but some of the worst ones actually have really really tiny pins believe it or not. I have a really tiny jar no bigger than my thumb with just scraps of cardboard lasagna-style I inoculated with mycelium and some spore solution. It's been sitting for 2 months in the dark. The growth is pathetic, but believe it or not there is a super tiny pin popping up from a primadora. Literally the tiniest pin I've ever seen. IDK if this could ever fruit or not, I can't imagine it growing big, but maybe there is some way to use this miniature pin to an agar at some point idk. Maybe I should try a random jar with just some cardboard at some point for a slow maintenance-free method. I've like never even had to water it, it's just damp.

And to the question about the capillary tube, lmao, no it's a miniature milk carton straw. If I can get some pics up I think I should because some of these setups are so bad but good u wouldn't believe it unless you saw it. Once I get more experience I'll set up mini trays and mini monos. The dirty tek takes some trial and error first. I don't want to waste a ton of soil.



As far as I know, this site removes anything traceable from the picture we upload :strokebeard:

Ahh I see, well as long as you are enjoying it and willing to do the work, then I think you should keep at it, I think it's going to be a long hard road for a few mushrooms at best, but I really don't know shit about doing this any other way then what I've learned here, so I could be wrong :muppet:

I wish you the best of luck and hope you keep us posted on the progress :rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29351953 -

Okay, try pf-tek then :shrug:

I grow cannabis in organic super soils, there are no significant parallels to mushroom cultivation.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk]
    #29352056 -

Damn, so probably need to incubate :frown: that's alright I got a seed Matt and I can just prop the tubs up with jar lids


--------------------
I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

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Re: Official Copelandia/Panaeolus Thread [Re: el boso] * 21
    #29352245 -

Weza finishing out its second flush..


I love days when all the trays in a tub are doing something I can stare at lol

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29352604 -

Hello! I collected some cow poop patties from a sanctuary, it is totally dry, super light and without smell. Is it okay like this or should it have more humidity?

I have a Pan. cyanescens "Estero" spore print to work with, right now I'm waiting for the spores to germinate.

I am a little lost at finding the right path for me tho. I have a grow tent with air exchange and humidity control that I use for gourmet, I think it could work, what could be a good first grow path for me? Could you share a link to updated teks for the species I have? This thread has too many posts it's hard to read and gather information 😪

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29352669 -

fahtster said:
Weza finishing out its second flush..


I love days when all the trays in a tub are doing something I can stare at lol




:chefskiss:


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Copelandia/Panaeolus Thread [Re: Muffa] * 3
    #29352681 -

I would start with liquid manure pasteurization for your manure.

This thread is for fruiting in monos.  But I still would recommend reading the OP for an overview on agar, substrate, and casing.

A couple of tent focused guides I would recommend are Asura's and Jake's.

For more information, teks, and growlogs, the guide in my signature is essentially an index.

Personally, I run my dreamers (automatic tubs) inspired by Jake's tents.  Fogger on a timer, to condense mist onto the fruits, followed by exhaust to promote evaporation.  My swings fluctuate from 90-80 rH on a 10 off/2 on cycle with a dehumidification cycle on the exhaust fan.  My temperature sits around 77f.  And I keep the surface humidity high until I see knots or first flush pins using either a flipped lid or grass so I don't have to hand mist or mess with the cycles.  I'll do a write up sometime soon, still ironing out a few bugs.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Muffa] * 3
    #29353186 -

Muffa said:
Hello! I collected some cow poop patties from a sanctuary, it is totally dry, super light and without smell. Is it okay like this or should it have more humidity?

I have a Pan. cyanescens "Estero" spore print to work with, right now I'm waiting for the spores to germinate.

I am a little lost at finding the right path for me tho. I have a grow tent with air exchange and humidity control that I use for gourmet, I think it could work, what could be a good first grow path for me? Could you share a link to updated teks for the species I have? This thread has too many posts it's hard to read and gather information 😪



https://www.shroomery.org/forums/showflat.php/Number/29144450#29144450

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Re: Official Copelandia/Panaeolus Thread [Re: Bra] * 3
    #29354385 -

My pan cyan jars look clean until it gets close to full colonization, these pics are jars shaken twice and now have been consolidating for a week or more. Do they look ok?




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Re: Official Copelandia/Panaeolus Thread [Re: Floret] * 1
    #29354419 -

They look great!

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 6
    #29354474 -



These are some TTBVI from the batch of prints in my giveaway.  This mother plate looked clean, so I sent some of to millet and also made some transfers.  Millet grew out well.  Sent to pasteurized sub in a mycobag.  Thought it looked ready today to send to fruiting chamber and damned if it isn't already pinning!  Just broke everything up, put in chamber and cased with 50/50 peat/verm pH9 with lime and caco3.  These mofos are quick.  Spore to pins in 25 days is the fastest I've gotten so far I think.  Of course I destroyed the pins, so will be a few more days before tray pins or I may have messed it all up.  Time will tell.


--------------------
I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 5
    #29354481 -

Thanks tree frog and Bra! Today the plate I dropped the spores on shown signs of germination


I'm soo excited for my first grow of pans 😍
I've been only growing APE for the last 2 and half years 🫠

Edited by Muffa (09/24/25 06:40 PM)

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