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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93]
    #29345197 -

I'm looking at BOD videos abt preparing your SAB / innoculating spores & such. I've seen the plastic wrap is inside the SAB. Why is it OK despite not being sterilized ? It's because we don't want an airflow and that's all ?


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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Anonymous]
    #29345200 -

Is it normal to have mycelium grown in your LC jar?

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29345213 -

Also, from https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888 about cleaning condensation.

He writes you should put a cup of warm water.

Do you put the cup of warm water in the sab / outside the SAB ?
Do you need to sterilize the cup ?
Do you need to wrap your dishes before doing so ?
If it's outside the SAB, can you safely put it back in the safe, open it, and inoculate ?

Thanks a lot everyone for the answers i've already got and i'll get :smile:


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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29345243 -

tryptkaloids said:
sloug said:
saran wrap for single plates is ridiculous. get yourself a roll of this and save yourself the hassle...



      :sunny:
:boombox2:


I have 2 rolls sitting there disintegrating



It's weird I've had my box for about a year and I don't do anything special to store it.

cuz I didn't know i was supposed to. There wasnt  instructions or anything like that on the box.

So yeah it just sits open air in my bathroom that's not temperature controlled.

Right now it's been sitting at about 80. It was temperature controlled for a while and would sit at 75. Anyways I guess I'm getting really lucky cuz it's not disintegrating and still works perfectly.

PS. this isn't an argument for either team. Just pointing it out that for some reason I've got a very special roll I guess lol.



My one huge pet peeve with parafilm was it was really hard to peel the paper backing off of it. But lotkid came in with the golden tip on that one and now it's not an issue.

However price is a pretty big factor. It's super expensive. But if you store it properly those rolls are pretty big and last quite a long time. Especially if you cut pieces off properly and get the most out of it. don't waste.


@tropical
When it comes down to it Saran wrap  and grafting tape to just as good of a job and are much cheaper.

So it's up to you. your choice. Pick a team. just be aware of whichever side you pick you're going to get extreme hell from the other lol


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29345256 -

So, after searching for "how long should i let my SAB settle before using it", i'm seeing various replies and ideas about this subject. Some don't wait, some wait a hour, some spray water + soap, some don't. What's the general concensus from TC members ?

Sorry for the load of questions, i'm trying to educate myself the best possible and not fuck up everything.

Ty


--------------------
wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29345258 -

Personally I spray wait a minute and get to work


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr] * 4
    #29345289 -

As of a few days ago I'm using no wrap on my plates. And im lovin it.  Talking about the Holy Grail screw tops baby!


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr] * 1
    #29345294 -

cronicr said:
Personally I spray wait a minute and get to work


I think my imagination just went someplace it wasn’t suppose to go…

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29345295 -

So I'm making the switch to agar and self-prepped millet jars. I did ~18-hour soak on the millet, drained 15m in a paint strainer bag, loaded wet, and 120m PC cycle (mix of pint and quart jars). I put a 1/4-plate wedge of what seemed to be a clean T1 transfer of RustyWhyte from a clone plate into this pint jar on 9/7, so it's now 8 days later. It's not that the growth is stalled, it does seem to be growing each day, it just seems very fucking slow, which is worrying me. I have some PE that I also put to grain about the same time which is also slow, but it's PE so slow feels normal. But RW doesn't seem like it should be super slow? (I wish I had remembered to take pictures of the plates before I put them to grain)

Should I be worried or STFU and LITFA?



--------------------
:blackspy:00010010
"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."

The Hitchhiker's Guide to the Shroomery <-- This is the way.



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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical] * 2
    #29345299 -

Check the SAB for spiders(I work in the basement), load the SAB, wash my hands and arms up to the elbows, dry with paper towels and get to work.

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29345301 -

Ty as usual.

To sterilize an agar bottle like from BOD agar tek, he states he likes to put the water at the level of the agar.

Do you use 3 quarts water and rise the trivet to reach that level, or just enough water to reach the agar with the trivet at the bottom of the PC ? (With a presto 23 qt)

ty


--------------------
wow

Edited by Tropical (09/15/25 07:43 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #29345307 -

mushboy said:
Tropical said:
If I close every windows for a hour to pour agar, can i prep agar meanwhile on my gaz stove in the same room ?


thats a really good observation.

imo i would not use a sab if i had a gas burner going in the same room at the same time.




I had always wondered this about people using their big blow torches right next to the sab. But lots of people do.

I'm always still worried about it but I have that thing running constantly next to my side while I'm doing work.

And for the most part no issues. 

I don't remember who but it might be Stipe or maby bod. Idk.  Somone used his blowtorch like directly in front of his sabs. almost right next to one of the arm holes. I always thought isn't that causing lots of turbulence?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


Edited by callmehippie (09/15/25 08:04 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29345312 -

I sterilise my agar in an IP but I have never once filled the water level up to the level of the agar and it has never caused me any issues :shrug:

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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
    #29345314 -

Dkeppel said:
I sterilise my agar in an IP but I have never once filled the water level up to the level of the agar and it has never caused me any issues :shrug:


:whathesaid:


Just make sure your caps on the bottles are loose. And that you Let The Vessel depressurize completely naturally before removing the lid. You shouldn't have any issues with boil over.

Well one dont use an ip. But same deal.


I actually just did a run with 15 mm deep pp5 dishes. And I put them directly in my PC no foil no canister nothing just 60 plates loaded into my pc. Those didn't boil over. So I definitely wouldn't worry about a media bottle.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29345316 -

After PC'ing agar for the pasty tek, do you have to wait for the condensation to evaporate before inoculating your pasty plates? There is quite a bit of condensation in them. Will they be alright if I wait about a week before inoculating them?

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Re: Ask Quick Questions, Get Quick Answers [Re: SexxLaws]
    #29345320 -


the answer to your question is yes LOL.

You don't have to wait. But you can if you want to. with no issues.


Lots of us use a few immediately if we need to. And then the rest gets stored away for multiple weeks on end. depending on how long it takes to go through them. While they're being stored the condensation will slowly go away.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29345326 -

callmehippie said:
As of a few days ago I'm using no wrap on my plates. And im lovin it.  Talking about the Holy Grail screw tops baby!




--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: SexxLaws] * 1
    #29345330 -

Condensation is mostly a visibility issue.

It makes it harder to view the plate or to take good photos but it is otherwise not detrimental.

You can immediately inoculate Pasty Plates once cool or you can leave them for a week or 2 or more, it will not be an issue unless the agar dries up.

How long until they dry up? That depends on thickness, enviroment, storage method etc.

I've never had uninoculated plates sit around for more than 2 weeks before using them but I have left inoculated plates in a cool, dry and dark place for about 18 months and the cultures were a little slow to take off, but they did eventually.

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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel] * 1
    #29345376 -

GenesisCorrupted said:
callmehippie said:
As of a few days ago I'm using no wrap on my plates. And im lovin it.  Talking about the Holy Grail screw tops baby!





:whatnonono:
I have never wrapped a HG plate, and I never will. Team no wrap for life!

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Re: Ask Quick Questions, Get Quick Answers [Re: JW123] * 1
    #29345391 -

One word, cats, it's impossible to imagine not having stuff wrapped.


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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