bluespirit13 said:

Let’s say I wanted to go from a clean agar plate, and be able to use it for PF tek.

What’s the easiest way to do so? Myc slurry in a syringe?

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drop some wedges from that plate directly into the pf jar. maybe push it down past the verm with a sterile rod of some sort. otherwise yea, some sorta liquid inoculant. i like making lc. if u put a port on it u can use it at ur discretion accross many jars

i mean i reeeally like making lc...

RoscoeReturns said:

I don’t give a shit about genetic diversity and pheno hunting. I want to spread out my spores so if there is contamination I have a better chance of finding a clean spot to transfer away from/less chance of having multiple organisms growing on top of one another. I think for the majority of people on here asking questions that is probably a more important goal than taking as much genetic diversity to grain as possible.

For the few hardcore hobbyists that already have a pretty clean workflow, and want to mess with genetics, you have a point. I just don’t think most people are there yet, or like me, will ever get there.

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However I believe Tao. Was explaining that the whole dilution thing is beneficial for bacteria and bacteria only. Not for separating clean colonies from bacteria. Its for streaking bacteria and studying it. So possibly that's how that kind of gets mixed to people's explanations as to why. Cause when reading the about "T" method in the link posted on this site. It dosnt really specify wjat application it being used for in an actual lab. Ie bacteria.


Or I could just be talking out of my ass. It's early this morning LOL

Either

I do the streaking and probaly I feel like most around here do it  for the reason Roscoe said exactly. But I don't do all the multiple flaming and stuff like that. I just simply do one zigzag down the center of the plate just to spread out what's clean from not clean just a tiny bit.

This way it's less complicated. You only lift the lid once and torch once and it's noob friendly.

Like this.

callmehippie said:

I guess it depends on how you look at it. I see it as ask quick questions get quick answers like quickly if you need help quick go ahead and ask you a question and you can get a quick answer.

Not short questions/short answers.

I mean sure it's nice to keep things as short quick and easy and too the point as much as possible

But some things take more explaining. You don't want to be cut off. then. might not be able to actually answer someones questions appropriately.

And there's more way to skin a cat. I love multiple viewpoints. You get to see how everybody is doing things and what might work for you in your situation and what won't.

Not everybody is going to have the same answer for every question. Especially all the TC's around here. A lot of times they tend to have different answers and even  disagree with each other. And that takes explaining.

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Nichrome said:

This is a place to ask quick questions. They happen all the time and this is a dedicated place.

Try to keep questions brief yet include as much detail as possible and try to keep answers brief and to the point.

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The Tao said:

Nichrome said:

This is a place to ask quick questions. They happen all the time and this is a dedicated place.

Try to keep questions brief yet include as much detail as possible and try to keep answers brief and to the point.

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This is from the opening post. Try and keep it brief but details are important. I check my responses for rambling before pressing the send button.
Concerning the flame, streak, rotate, repeat pattern. Try it out by swabbing the palm of your hand or a kitchen sponge to a plate then flame, rotate and repeat with a loop. Pretty interesting what will grow. It’ll demonstrate how the bacteria will be easier to isolate and identify.
It’ll also make you want to wash your hands and toss that sponge.

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