PuffM4gicDr4gon said:

I wonder if there are similar looking mushrooms to woodlovers that grow in the same area that interfere with motor neurons? WLP could be as simple as people eating a misidentified mushroom and suffering side effects because of it.

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Mub said:

Finally getting around to prep some coir today. I am using pint jars to go into a 6qt shoebox. Someone in this thread said

you want about 80g/quart for spawn, about 20g for a top layer.

The tech I was following suggested 550 g should be enough for about 6 of theb6 qt shoeboxes. What would be best?

Edit: just realizing I think I misunderstood the 8/ qt.  Subs meant to be per qt of spawn I was think pert quart of the tub.

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I don't know if this helps but honestly I've never measured coir.

If you're sitting around with everything open in your kitchen waiting for an answer, you're not going to wreck anything by mixing up some shroom jars mycelium with some coir and some vermiculite. (some people skip the verm)

Verm and coir aren't nutritional, they're mainly to hold water and give a place for mycleium to grow. Nutrient is the grain.

Just mix in enough water to get to "field capacity." When you squeeze a handful you should get a series of a bunch of drops. If a steady stream, you have too much water, add verm (or coir if that's all you have)> If just a few drops, add water.

dinosaurs said:

this video, linked from this paper on oregon.gov, goes into some of the data and hypothesis concerning WLP.

interesting is that some of the compounds found in woodlovers produced symptoms attributed to loss of motor skills in biological endpoints in laboratory conditions.

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Again, that is an opinion piece and not a study. 

Pretty sad that people have to keep posting the exact same link over and over due to lack of proof.

“Researchers have come up with a number of elaborate hypotheses over the years, based on
anecdotal evidence and scientific extrapolations, in an attempt to explain this strange and little-
understood phenomenon. None of them have gotten very far.”

Sounds like they can’t prove it’s existence, according to the link you posted

MN_John said:

Question regarding sealed tubs for culturing.

I've got two sealed Sterilite 7.5 quart tubs going with about 3lbs of spawn (all white) and 5lbs of substrate (purchased, pre-sterilized) split between them. All done in a still air box, no signs of contamination. These are being kept in 75° controlled space.  Its been 10 days and no signs of additional colonization.

Now my question, do I need to provide any FAE for these tubs to colonize the substrate?  Or just leave them alone until they turn all white?

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nektar61 said:

I have zero experience with wood lovers.

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MN_John said:

Question regarding sealed tubs for culturing.

I've got two sealed Sterilite 7.5 quart tubs going with about 3lbs of spawn (all white) and 5lbs of substrate (purchased, pre-sterilized) split between them. All done in a still air box, no signs of contamination. These are being kept in 75° controlled space.  Its been 10 days and no signs of additional colonization.

Now my question, do I need to provide any FAE for these tubs to colonize the substrate?  Or just leave them alone until they turn all white?

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nektar61 said:

Grow tubs/ shoeboxes should NOT be sealed.

I put my lids on upside down to allow a crack of air to get in, that's enough. But not sealed.

75 F, you're not heating it are you? no need, and increases chance of contam. I shoot for 70, but can be hard late spring/ summer. 75 is fine, just don't heat to that.

Has to colonize whole thing then you'll see pins (little shrooms)  But with no FAE in sealed container, and no moisture, is going to take way too long, if happen at all.
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Let get to room temp, and mix some of that coir into the tub with a little water. I'd still do that if I were you. My sig line "from spores to trips" recommends the two brands of coir I'd get. You can probably get it from Amazon in 2 days. Read my part in that about coir.

(Coir pretty much need, verm I use but some don't).

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MN_John said:

I'm using North Spore Boomr Bag, it contains "composted horse manure, coco coir, and several quality substrate supplements".

My grow area is about 65°, so the tubs are in a lined box with a couple of seeding heat mats.  I'm distributing the heat so the tubs aren't getting to much spot heat, all readings are under 80°.

So how do I prevent contamination of the tubs if they aren't sealed and the mycelium hasn't taken over yet?  I thought high CO2 was good at this stage?  This is my first grow.

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OK, don't need coir or verm using that, was just saying for next time.

Sounds like you might need water and you for sure need air flow.

"CO2 before shrooms" / separate fruiting conditions" is 20 year old voodoo / bullshit that is not best practices anymore.

If spawn is clean, it's almost impossible to get contam at this point. The spawn should out compete normal stuff in the air. UNLESS YOU'RE ADDING HEAT. DITCH THE HEATING PAD. NO ONE HERE USES THAT.

You're much better off growing at 65 instead of adding heating pad. heating pad will get bacteria in bottom of tub at worst, and shrooms growing on the bottom of the tub at best.

And...Give those fuckers some air flow! You're suffocating them.

If it's dry, add some water. See my post above about Field Capacity.

Do you have any pix of your Petri dishes (or whatever you did before jars), plus the jar before you put it in tub?

Curious for the record, where did you hear these? They're all wrong. I'm wondering Reddit, old book, masked idiot on YouTube, or what? (nothing wrong with being wrong at first, we all did that)
--heating pad
--Sealed tub / CO2 before pins

And did no one mention water? Or is the boomer bag really wet? I've never used one. 

What grain did you grow your spawn on? What did you start with? spores on grain? Spores on agar? Walk me through it, since you included no pictures.

For future, you can save money, you don't need a boomer bag. It should work but shrooms grow as well on grain as on grain and poo, with less problems. I use birdseed in jars, let spawn in jars for a month, then mix with coir, verm, and water, that's it.

Costs me maybe a dollar to grow an ounce (dried) of shrooms.

Welcome.

nektar61 said:

OK, don't need coir or verm using that, was just saying for next time.

Sounds like you might need water and you for sure need air flow.

"CO2 before shrooms" / separate fruiting conditions" is 20 year old voodoo / bullshit that is not best practices anymore.

If spawn is clean, it's almost impossible to get contam at this point. The spawn should out compete normal stuff in the air. UNLESS YOU'RE ADDING HEAT. DITCH THE HEATING PAD. NO ONE HERE USES THAT.

You're much better off growing at 65 instead of adding heating pad. heating pad will get bacteria in bottom of tub at worst, and shrooms growing on the bottom of the tub at best.

And...Give those fuckers some air flow! You're suffocating them.

If it's dry, add some water. See my post above about Field Capacity.

Do you have any pix of your Petri dishes (or whatever you did before jars), plus the jar before you put it in tub?

Curious for the record, where did you hear these? They're all wrong. I'm wondering Reddit, old book, masked idiot on YouTube, or what? (nothing wrong with being wrong at first, we all did that)
--heating pad
--Sealed tub / CO2 before pins

And did no one mention water? Or is the boomer bag really wet? I've never used one. 

What grain did you grow your spawn on? What did you start with? spores on grain? Spores on agar? Walk me through it, since you included no pictures.

For future, you can save money, you don't need a boomer bag. It should work but shrooms grow as well on grain as on grain and poo, with less problems. I use birdseed in jars, let spawn in jars for a month, then mix with coir, verm, and water, that's it.

Costs me maybe a dollar to grow an ounce (dried) of shrooms.

Welcome.

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MN_John said:

I'm using one of North Spore grain bags injected with spores.  The bag cultivated just fine. I thought I would worry about saving money after getting a couple ounces of supply saved up. This area of my house is pretty cool, so I needed to add some heat. The Boomr bag seemed to be at field capacity to me, it was a fresh one and a couple drops squeezed out. 

One of the tubs I did a straight lasagna layering, the other one I mixed it up well.  I'm still worried about contamination. I guess I probably need some FEA, really sounds dangerous at this point.

Once I get pins I'm putting these tubs in a monotub with a fan on a timer and humidity control.

Thanks for your continuing advice.

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Happy to advise.
If only a couple of drops came out when you squeezed the boomer bag, mycelium will need more. I'd add 3 or 4 ounces of water per shoebox tub.

You can add it now. Just pour it evenly on the top, and that's it.

Don't worry about contam. If your bag is clean, you don't need hermetically sealed to grow from there. These things grow on cow shit in the open air and people eat those. haha.

Air is not dangerous to clean spawn. Again, where did you get this info? I always like to know where the b.s is coming from.

You do NOT need "a fan on a timer and humidity control." You are WAY overthinking this.

No one here uses that. A tub gets enough air through the crack of the upside down lid, based on the tiny amount of heat that spawn produces. Makes little air currents. If you stick a temp probe in there (once you turn off your heater for a day), you'll see it's about 5 degrees warmer than air temp in the middle.

Humidity will be right in the tub if sub is moist enough. If it's not moist, you can mist it with more water in a week, depends on how dry in your house.

Healthy shrooms aren't a dying infant on life support. No machinery needed. (unless very cold in winter, then I put a small 750 watt heater in the room, heating the room, not heating the shrooms, and put THAT on temp control. Only comes on a few minutes per hour in the walk-in closet I use as a shroom room. Has a blanket over the doorway)

Heating pad creates a micro climate of too warm where it touches the tub and the rest of tub is too cold.

You can grow shrooms at 50 F, it will just take longer. If you can't afford to heat the room, do it cooler and be patient.

They'll grow fine at 66, 67, 68, any room temp you can stand they will do well. More heat above 70, especially on one spot on tub, means more chance of contam.

If you heat a closet, add a smoke detector.

Two 16-quart tubs (2x size of shoebox each) full of shrooms grown exactly as I'm suggesting.

dinosaurs said:

lol someone should train a LLM on mr piggy's post history, give it a shroomery account and watch him argue with himself until he burns out.

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You’ve got a negative rating and multiple blocks in two months time.  You give out poor grow advice and argue with the mods about the forum rules.

You’ve never done agar and post in advanced mycology.

Post through it buddy, maybe if you do it enough you’ll be a real cultivator and not some guy who can only squirt vendor lc into rice.

dinosaurs said:

they need next to no FAE during colonization, and in fact I've seen data from commercial mushroom grows that points to colonization going faster in a high co2/low o2 environment..

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Mr Piggy said:

I will probably die on a hill.  A hill of righteous correctness.  That or skulls, skulls would rule.

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Every time I see the term “triggering fruiting conditions.”

Mr Piggy said:

dinosaurs said:

they need next to no FAE during colonization, and in fact I've seen data from commercial mushroom grows that points to colonization going faster in a high co2/low o2 environment..

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Like, what the fuck is this bullshit?  Why would you say this unless you have no experience?

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