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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29228379 -

:whathesaid:

After two weeks I start to question, after a month I get worried, after 2 months I'll use that plate for a transfer (if I'm low on plates.)

Spores^

Wedges should be at least fluffing up

Edited by LewDoja (05/25/25 11:29 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Changed] * 1
    #29228401 -

No growth from wedges? What in the agar and what’s the actual temperature?

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29228424 -

Its Burma. Never grown it before. In an act of naivety I scooped up a couple different strains not realizing different strains are...different and may need different levels of care, i guess.

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #29228430 -

A cube is a cube for the most part when growing, the difference is that PE and its variants are basically so genetically modified that they react different, and take longer to grow.

Different species like Pans, Subtrops, or Gyms will require different levels of care.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Changed]
    #29228447 -

All cubensis?

They need more time, no stress my friend:rockon:

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Re: Ask Quick Questions, Get Quick Answers [Re: Puduwoke] * 1
    #29228467 -

Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #29228613 -

Ran my brown rice jars through the instant pot on high for 2.5 hours last night. Today I read about venting all of the air out first. I definitely didn’t do that. Should I just dump these jars and start over?

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Re: Ask Quick Questions, Get Quick Answers [Re: Potion_Seller]
    #29228618 -

I would. Easy and cheap to start over. In my opinion it's not worth the risk.

Might be worth waiting for someone with more experience to answer though :shrug:

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Re: Ask Quick Questions, Get Quick Answers [Re: Potion_Seller]
    #29228647 -

if you pc'd them for 2.5 hours they're good to use.


I do 135 minutes for minute.

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Re: Ask Quick Questions, Get Quick Answers [Re: Potion_Seller] * 4
    #29228660 -

If I didn't vent before running a cycle on an iffy psudo-pressure cooker like an IP then I would throw out my grain and start over.  Venting is very important and it's not worth wasting all your clean work on iffy grain.  Just run another cycle and vent properly.  If you are capable in the future, get an actual pressure canner (NOT a pressure cooker) and run some real grain like WBS, oats, wheat, milo, ect.  You will not regret the expense.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 2
    #29228662 -

Mub said:


Are these ready to go into a tub? Already been shaken once. Have four like this that all look like this all the way around seems somewhat consistent.


I'd send it.  Millet often looks like it has uncolonized grain when in fact the whole thing is colonized.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Potion_Seller] * 5
    #29228685 -

Potion_Seller said:
Ran my brown rice jars through the instant pot on high for 2.5 hours last night. Today I read about venting all of the air out first. I definitely didn’t do that. Should I just dump these jars and start over?



Proud of this one: To vent, or not to vent? - Comparison

TL;DR:
Table by the courtesy of ReverendMyc:


--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

Edited by MycCycle (05/25/25 06:09 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: MycCycle] * 1
    #29228686 -

MycCycle said:
Potion_Seller said:
Ran my brown rice jars through the instant pot on high for 2.5 hours last night. Today I read about venting all of the air out first. I definitely didn’t do that. Should I just dump these jars and start over?



Proud of this one: To vent, or not to vent? - Comparison

TL;DR:
By the courtesy of ReverendMyc:



Also don't Insta Pots only reach like 12-13psi so you'd need to run for longer than 2.5hrs?


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29228696 -

That's right


--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

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Re: Ask Quick Questions, Get Quick Answers [Re: MycCycle] * 1
    #29228723 -

Thanks! That’s really good info. I’ve dumped the rice out. I’ll run this batch tonight for longer too.


--------------------
IDK what I'm doing yet. Be nice to me. I search a lot I swear.

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29228733 -

I can only convey my own experience, but I've run over 100 jars through my instant pot for 2:15 and I've never had any issues.  however I use brown rice which is thought to have a lower inherent endospore count than some other grains.

the process that I've settled on and that has produced consistently good results:

-boil water on the stove.
-when its rolling boiling, chuck the brown rice in.
-boil rice for 9 minutes.
-immediately cold rinse the rice well to de-starch.
-drain rice well and and load it into jars using the lid methodology of your choice.
-cover tops with foil and PC 2hr 15min in the instant pot with natural pressure release.
-after the pressure lock releases, immediately pull them out and shake them well to break up any residual clumps. 
-inoculate them the following day.

follow that and you'll have lovely jars of nice loose rice that the mycelium will love to chew on :smile:

Edited by dinosaurs (05/25/25 07:14 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Potion_Seller] * 2
    #29228734 -

I read through your posts. You’re intending to use a vendor LC syringe to inoculate grains and make new LC jars with an instant pot without testing the vendor syringe on agar?
How will you know where or how any contamination happened?
What tek are you following?

Edit: Bad grammar

Edited by The Tao (05/25/25 07:10 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29228782 -

Where are y’all buying millet? I can’t find it anywhere. I’d love to try it out since people say it gets faster colonization and the prep seems easy af, but nowhere has it in bulk. I can only find those bird treat millet stick things…

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Re: Ask Quick Questions, Get Quick Answers [Re: ElGringoGrande]
    #29228788 -

ElGringoGrande said:
Where are y’all buying millet? I can’t find it anywhere. I’d love to try it out since people say it gets faster colonization and the prep seems easy af, but nowhere has it in bulk. I can only find those bird treat millet stick things…


Feed mills, ask for white millet.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29228792 -

The Tao said:
No growth from wedges? What in the agar and what’s the actual temperature?


The Tao said:
No growth from wedges? What in the agar and what’s the actual temperature?


Golden Light DME, agar agar, water.

The wedges were kindly gifted from a user here, and they were quite well smashed in transit, so I took just a scrapping with a scalpel and transferred it.

Even now, it gets down to the 30's Fahrenheit at night here, and I keep my window open, so its probably in the 50s at night, and barely scrapping 70 outside during the day. Barely any humidity...

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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