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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: fahtster]
    #29206553 -

fahtster said:
Laugh all you guys want.  I put this culture through one cabin and grew a banging tub



Great to see ya come to the clear side! I wasnt denying the magic of cabin sequestering, just stating what id do.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: tryptkaloids] * 11
    #29206675 -

I feel like it’s just the name with smelly.  Like if it was dubbed “updated agar sandwich method”, it’d be aight :lol: 

I just see it as a huge time saver.  Why spend weeks trying to get clean growth through eyeballing transfers when there’s a really good chance you’ll clean it up in one?

Im running everything through a cabin.. clones I take that look clean, ms, everything.  I’m looking for great genetics.  If there’s even a chance that something is slightly baccy and that hinders great genetics from showing their true potential, why risk it.. for me personally, it’s a no-brainer.  Is it a cure-all? No, but from what I’ve witnessed from doing a pretty large amount of them, it’s a great technique and I think my future grows are gonna show that.  :shrug:

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: fahtster] * 6
    #29206693 -

I just think it’s funny. Not people using it, but that some lab guys figured out a technique people have been using for years and renamed it to something silly.

I’m pretty sold on its efficacy for those in need. Personally I’m not sure how much utility it has for my grow? Maybe I need to build some cabins for my cultures that insist on microfruiting. Might help them along?

Actually, scalloping aside, I have some things I could CS process to see if they improve. Maybe I’ll give that a go 👍

I hope no one sees me as a legitimate hater. I mean I hate the name, yes. You can see me as a hater of that.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: fahtster] * 3
    #29206740 -

Im way onboard smelly.
So much this
fahtster said:
I feel like it’s just the name with smelly.  Like if it was dubbed “updated agar sandwich”, it’d be aight :lol: 




For me it ultimately boils down to a big pet peeve of mine in this hobby. Renaming processes that already exist.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: tryptkaloids] * 2
    #29206758 -

Then you’ll be very interested in my island sequestration technique with agar :lol:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: Mr Piggy] * 3
    #29206765 -

I did come up with a truly revolutionary new tek. It’s called “Rapunzel Sequestering,” or “Fiona Sequestering,” depending on how cultured you are.

Basically you use a tall half pint, build a tower out of agar, plate your transfer at the top, and look for rhizo growth down the sides of the tower. Once it reaches the bottom, you can then take your sample and move it to the next plate.

Which will be a moat sequester. I think you can guess what that might be.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: Smellyhobbit] * 4
    #29206877 -

Well, technically it’s not really a sandwich, more like a toad in the hole :shrug:


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Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: 3.A.M] * 4
    #29206887 -

You’re a toad in a hole


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: Smellyhobbit] * 3
    #29206934 -

Toad in a hole it is!


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: Smellyhobbit]
    #29206938 -

I’m into this hobby solely for the purpose of playing with genetics lol. Getting to the point where I can.

So for the most reliable crosses you’d want mon-mon mating? My ideal setup for me is gonna be diluting spores and sourcing monokaryots. Doesn’t seem extremely hard I think I can do that  by taking a spore swab. Then I grow out that mono and take a section from it and a section of a mono from another variety (sourced by spore)

This should create a dikaryot with genetics from both “parent fruits” correct?

I’m not worried about crossing varieties as much as I am selective breeding. So let’s say I have a fruit that typical colored and drops red spores and one that’s albino and drops clear spores.

The cross should result in a spectrum of fruits with varying genetics from both parties? At some point one that’s white with red spores should pop up, then you’d clone and print that?

Then you’d grow that clone and you should have all white fruits with red spores, and if you don’t you take the “parent albino red spore” mono and mate pair it with a fresh mono from the t2 and that should help “lock in” the genetics? More cloning and inbreeding may be needed?


I’ve seen threads of others saying they’ve done mon-di mating. Is this mixing a mono with a culture and hoping it mates? Can you do di-di mating as well? I’m assuming the accuracy and confirmation of crosses is easier when dealing with monos?


Genetically everytime a mushroom grows it creates spores which mesh the 2 genetics in the dikaryot mycelium into a mono? Assuming you’re gonna loose some genetics from both parents this way? So I may want to experiment with multiple monos from 1 mushroom?


--------------------
Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: misterswisher]
    #29206987 -

You should really just get some entry level fungal biology under ur belt.

Ed grand has some decent YouTube videos

Di-mon mating is also known as buller phenomenon and the dikaryon donates one of its nuclei to the monokaryon

Di-di is probably not a thing but it could be in rare cases. But there are things that can happen where it would appear that two dikaryons mate

Have you read fahts thread, or muad.dweebs threads?


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

Edited by catnip40 (05/03/25 08:23 PM)

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: tryptkaloids] * 2
    #29207004 -

Mr Piggy said:
Then you’ll be very interested in my island sequestration technique with agar :lol:



Sounds like this might be comparable to my brand new “mycelium foxhole” agar method :lol:


tryptkaloids said:
fahtster said:
Laugh all you guys want.  I put this culture through one cabin and grew a banging tub



Great to see ya come to the clear side! I wasnt denying the magic of cabin sequestering, just stating what id do.



Faht do you still have that plate?? I’m pretty curious if whatever contam was in there would eventually travel through the mycelium over the wall or if it’s legitimately stuck in there.


--------------------
LAGM2020

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: A.k.a]
    #29207006 -

Nah.. reused it unfortunately.  I’ll lyk if I run into that situation again tho.  It’s a good question for sure

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: catnip40]
    #29207018 -

Was watching a bunch of Ed Grand videos and read fhats thread and a few of the others. That’s what inspired me to make that post. I prefer videos but I’ll read a thread if I have to. The format here hurts my eyes lol.

Honestly I figured this was the next stepping stone for me. I don’t document everything by here, and sometimes act like an idiot, it’s my ✨ personality ✨. But I’ve got a good amount of Petri transfers grows observing and identifying mycelium and basic mycology under my belt. Most my experience is specific to cubes but I also have some biology background  and I figured messing with the genetics of some cubes really was the next step for entry level fungal biology unless ur saying I should work with another non active species to learn?

The red spore thing was merely just an example. My goals aren’t so ambitious right now just getting into it lol. But that is how it works right? At least when ur mating spore germinations? Is is plausible for me to do that with proper technique and spore dilution?


--------------------
Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

Edited by misterswisher (05/03/25 08:44 PM)

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: misterswisher]
    #29207028 -

Fuck that guy.

The one time I came across something from him it was him trying to claim serial dilution as a new breakthrough called “the trademarked Dr Ed method”. Then when I said it wasn’t new he freaked out.

I get these guys have to constantly rehash shit to create more content but come on.


--------------------
LAGM2020

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: misterswisher]
    #29207029 -

yes ur on the right track.. pastys thread about RW is a good one. ed grand dilutes by just streaking look for grab n drag tek or something. ed just streaks in a fashion that produces monokaryon colonies

alan has a good video about serial dilutions


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: A.k.a] * 1
    #29207033 -

A.k.a said:
Fuck that guy.

The one time I came across something from him it was him trying to claim serial dilution as a new breakthrough called “the trademarked Dr Ed method”. Then when I said it wasn’t new he freaked out.


i mean. theres not much videos about this stuff, its good to see demonstrations. but ya ed is a goofball but that doesnt mean he doesnt know what hes talkin about either

i meme on ed all the time but i can still respect the knowledge

bro was probably joking tho i think everyone even noobs are aware serial dilution has been done since like the 1800s so lol either way. he does have decent videos that show examples instead of reading it which is what the op said is preferred


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: catnip40]
    #29207053 -

Yeah I’m gonna read the RW thread completely and a lot more into these breeding projects for some reason i couldn’t find that stuff through the search. Not until I seen it linked on recent threads or to me. Definitely searched for it.

The video I watched he used a surfactant and water to dilute the spores over and over until it was a very small amount and used a grid on his dishes. That seems so fool proof and doable in a SAB to me (I’ll be using small jars with holes and fresh syringes) everything should be able to stay very sterile. But yeah thanks for the confirmation I guess reading coulda gave me that but rewording stuff helps me learn stuff if that makes sense.

I got a few cultures going right now. I wanna incorporate enigma genetics like the enigma rw project. That inspired me and I’m sure there’s lots of funky genetics inside enigma. But I can’t wrap my head around getting a mono from that, besides doing something like Fhats thread was talking about. I have a theory with using Deamascus earth but its water soluble so the mycelium would need to be suspended in something that won’t dissolve it lol.

But I think to dip my toes in I’m just gonna take 2 fruits from my next grow with the biggest caps and collect monos from them. If I can successfully do that I’ll try to get them to clamp on a dish and see if the next generation has all big caps. And continue on until I get the biggest capped mushroom lol.


--------------------
Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: misterswisher]
    #29207131 -

I mean I don’t see how that would get you to big caps faster than just regular breeding :shrug:

Diatomaceous earth is not water soluble.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only Tc's Can Answer! [Re: Mr Piggy]
    #29207157 -

Yeah that was another bad example. And I swear last night when I was looking into it google said it was but you right so that’s a decent idea honestly. This shit gets my dick hard and sometimes I think with my dick and not my brain and end up stepping on it 😂 yeah just selecting the biggest caps would do that idk what I was thinking. I guess it’s more useful for crossing varieties.

Curious as to what genes are dominant and recessive. What mutations we haven’t seen yet lol. Are there lots of people just crossing any and everything and observing the results? I haven’t heard anything of hyper pigmented fruits and I bet that mutation is tucked away somewhere lol.


--------------------
Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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