Puduwoke said:

Is the grass to keep better humidity at the substrate level?

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It does that too.  Also gives beneficial microbes a home, such as Azospirillum, which this casing is inoculated with.  But very likely there are beneficial microbe endospores on the grass seed already.

Essentially, I do normal grows but I also experiment a lot with symbiotic relationships.  The control bag isn't doing anything.  And I need to trim the wheatgrass ones because it's very thick.  Of the four, only this one with perennial ryegrass is pinning.  All four have Azosporillum in the casing.

Edit:  One of the wheatgrass bags is pinning too actually, discovered this when I went to cut the grass.

Generic said:

I've asked this before, and i'm still confused so here I go...

...but natalensis and ochraceocentrata are two different species; which one are you talking about?

or is that an either/or thing?, like they're interchangable.  Do you see them as the same?

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I meant ochraceocentrata all along. Have been growing them since they were known as p. natalensis. The names are NOT interchangeable. The 'true' natalensis was sequenced and properly identified. (most likely Alan ). The fact that i call them 'fakenats' or 'ochernats' is out of endearment, nothing more. Anything else you would like me to clarify?

Cak Le Boof said:

These aren't the best pics, but do these areas look more promising for transfers?

Thanks a lot

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Go back to spores and start from scratch. Don't waste your time with those plates. Spend your energy into making sure your sterile technique is flawless rather than saving obvious contaminated plates.
Best of luck.

Cak Le Boof said:

I think that the syringe must have been bunk. I've made hundreds of cube plates with very low contamination rates, I never have more than a couple per 20 going sour.

The agar recipe is new to me which is a variable, but I extra carefully pc'd to make sure.

Unfortunately the syringe was the only purchase option I could find where I am.
Time to contact the vendor!

Thanks again.

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Agar is easily sterilized ...most fkery is caused by innoculant, and secondary is operator error, I'd even say damaged packaging or a botched sterilized sleeve is before that....

Well at least for anyone who's knows what they're doing